Overview
- Offers readily reproducible, step-by-step laboratory methods
- Features easy-to-follow recipes to promote successful results
- Includes sections on interpreting and evaluating results, as well as troubleshooting advice for each protocol
- Includes brilliant color photos showing the results of fluorescence in situ hybridization on various tissues
- Includes supplementary material: sn.pub/extras
Part of the book series: Springer Protocols Handbooks (SPH)
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Table of contents (57 protocols)
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Front Matter
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Required Equipment and Probes for FISH Procedures
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Front Matter
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FISH Procedure
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Front Matter
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Material Suited for FISH Applications in Humans
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Front Matter
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Keywords
About this book
This manual offers detailed protocols for fluorescence in situ hybridization (FISH) and comparative genomic hybridization approaches, which have been successfully used to study various aspects of genomic behavior and alterations. Methods using different probe and cell types, tissues and organisms, such as mammalians, fish, amphibians (including lampbrush-chromosomes), insects, plants and microorganisms are described in 57 chapters. In addition to multicolor FISH procedures and special applications such as the characterization of marker chromosomes, breakpoints, cryptic aberrations, nuclear architectures and epigenetic changes, as well as comparative genomic hybridization studies, this 2nd edition describes how FISH can be combined with other techniques. The latter include immunostaining, electron microscopy, single cell electrophoresis and microdissection.
This well-received application guide provides essential protocols for beginning FISHers and FISH experts alike working inthe fields of human genetics, microbiology, animal and plant sciences.Editors and Affiliations
About the editor
Dr. Thomas Liehr, a biologist (diploma, PhD and postdoctoral degree in human genetics), has been working in molecular cytogenetics since 1991. He has been head of the molecular cytogenetic group at the Institute of Human Genetics, Jena, Germany since 1998; before he was a PhD fellow of the Herbert Quandt Stiftung der VARTA-AG at the Institute of Human Genetics, Erlangen, Germany. Working intensively in diagnostics, since 2002 he has been a ‘specialist in human genetics diagnostics’ (FHG = Fachhumangenetiker), a title awarded by the German Society of Human Genetics (GfH), and a European registered Clinical Laboratory Geneticist (ErCLG) since 2015. Dr. Liehr’s research fields include clinical genetics, leukemia cytogenetics and the (3D) structures of the interphase and the human chromosomes, as well as breakpoint characterization. The results of his research have been published in 5 books, >50 book chapters, >550 peer-reviewed papers, and ~800 abstracts. His particular expertiseis molecular cytogenetics including chromosomal heteromorphisms, small supernumerary marker chromosomes (sSMC) and uniparental disomy, fields for which he has collected all available literature and made it freely available on the Internet. Further, Dr. Liehr has multiple longstanding international collaborations with many scientists around the world including researchers in e.g. Armenia, Croatia, India, Morocco, Russia, Serbia, Thailand, and Brazil.
Bibliographic Information
Book Title: Fluorescence In Situ Hybridization (FISH)
Book Subtitle: Application Guide
Editors: Thomas Liehr
Series Title: Springer Protocols Handbooks
DOI: https://doi.org/10.1007/978-3-662-52959-1
Publisher: Springer Berlin, Heidelberg
eBook Packages: Springer Protocols
Copyright Information: Springer-Verlag Berlin Heidelberg 2017
Hardcover ISBN: 978-3-662-52957-7Published: 27 October 2016
Softcover ISBN: 978-3-662-57100-2Published: 22 April 2018
eBook ISBN: 978-3-662-52959-1Published: 17 October 2016
Series ISSN: 1949-2448
Series E-ISSN: 1949-2456
Edition Number: 2
Number of Pages: XIII, 606
Topics: Cell Biology, Human Genetics