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Hydrocarbon and Lipid Microbiology Protocols

Activities and Phenotypes

  • Book
  • © 2017

Overview

Editors:
  1. Terry J. McGenity

    1. School of Biological Sciences, University of Essex, Colchester, United Kingdom

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  2. Kenneth N. Timmis

    1. Institute of Microbiology, Technical University Braunschweig, Braunschweig, Germany

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  3. Balbina Nogales

    1. Department of Biology, University of the Balearic Islands and Mediterranean Institute for Advanced Studies (IMEDEA, UIB-CSIC), Palma de Mallorca, Spain

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  • Offers readily-reproducible step-by-step laboratory methods
  • Provides helpful tips and tricks supporting the protocols
  • Includes troubleshooting advice
  • Contains practical laboratory guidance to promote successful results

Part of the book series: Springer Protocols Handbooks (SPH)

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Table of contents (15 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Introduction to Activities and Phenotypes

    • Florin Musat
    Pages 1-6

  3. Protocols for the Measurement of Bacterial Chemotaxis to Hydrocarbons

    • Jayna L. Ditty, Rebecca E. Parales
    Pages 7-42

  4. Determining the Tendency of Microorganisms to Interact with Hydrocarbon Phases

    • Hauke Harms, Lukas Y. Wick
    Pages 43-53

  5. Protocol for the Measurement of Hydrocarbon Transport in Bacteria

    • Jayna L. Ditty, Nancy N. Nichols, Rebecca E. Parales
    Pages 55-67

  6. Respiration Rate Determined by Phosphorescence-Based Sensors

    • Michael Konopka
    Pages 69-80

  7. Measuring the Impact of Hydrocarbons on Rates of Nitrogen Fixation

    • Florin Musat, Niculina Musat
    Pages 81-97

  8. Protocols for Measuring Activity of Sulphate-Reducing Bacteria in Water Injection Systems by Radiorespirometric Assay

    • Gunhild Bødtker, Terje Torsvik
    Pages 99-109

  9. Bacterial Solvent Responses and Tolerance: Cis–Trans Isomerization

    • Hermann J. Heipieper, Nancy Hachicho
    Pages 111-118

  10. Protocols for Measuring Biosurfactant Production in Microbial Cultures

    • Roger Marchant, Ibrahim M. Banat
    Pages 119-128

  11. Analysis of PHB Metabolism Applying Tn5 Mutagenesis in Ralstonia eutropha

    • Matthias Raberg, Daniel Heinrich, Alexander Steinbüchel
    Pages 129-148

  12. Microbial Control of the Concentrations of Dissolved Aquatic Hydrocarbons

    • D. K. Button
    Pages 149-166

  13. Phenotyping Microarrays for the Characterization of Environmental Microorganisms

    • Etienne Low-Décarie, Andrea Lofano, Pedram Samani
    Pages 167-181

  14. Laboratory Protocols for Investigating Microbial Souring and Potential Treatments in Crude Oil Reservoirs

    • Yuan Xue, Gerrit Voordouw, Lisa M. Gieg
    Pages 183-210

  15. Protocol for Evaluating the Biological Stability of Fuel Formulations and Their Relationship to Carbon Steel Biocorrosion

    • Renxing Liang, Joseph M. Suflita
    Pages 211-226

  16. Protocols for Measuring Methanogenesis

    • Oleg Kotsyurbenko, Mikhail Glagolev
    Pages 227-244
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Keywords

About this book

This Volume features protocols for investigating the hydrocarbon- and lipid-specific activities of microbes. They include methods for studying chemotaxis, the colonisation of hydrocarbon surfaces, hydrocarbon uptake, respiration, nitrogen fixation, sulphate reduction, membrane stabilisation through cis-trans isomerisation of membrane fatty acids, and the production of biosurfactants and biopolymers in response to the presence of hydrocarbons. A protocol for studying the ability of microbes to control the concentration of hydrocarbons in their aqueous environment is also described, and phenotyping methods to reveal microbes’ more general metabolic activities are presented. Several protocols for investigating acid production in connection with oil souring and biocorrosion by microbes in oil well, oil transportation and storage settings are presented. Lastly, protocols for measuring methanogenesis, as an example of microbial hydrocarbon production, are described.<

Hydrocarbon and Lipid Microbiology Protocols
There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.


Editors and Affiliations

  • School of Biological Sciences, University of Essex, Colchester, United Kingdom

    Terry J. McGenity

  • Institute of Microbiology, Technical University Braunschweig, Braunschweig, Germany

    Kenneth N. Timmis

  • Department of Biology, University of the Balearic Islands and Mediterranean Institute for Advanced Studies (IMEDEA, UIB-CSIC), Palma de Mallorca, Spain

    Balbina Nogales

Bibliographic Information

  • Book Title: Hydrocarbon and Lipid Microbiology Protocols

  • Book Subtitle: Activities and Phenotypes

  • Editors: Terry J. McGenity, Kenneth N. Timmis, Balbina Nogales

  • Series Title: Springer Protocols Handbooks

  • DOI: https://doi.org/10.1007/978-3-662-49140-9

  • Publisher: Springer Berlin, Heidelberg

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer-Verlag Berlin Heidelberg 2017

  • Hardcover ISBN: 978-3-662-49138-6Published: 25 November 2016

  • Softcover ISBN: 978-3-662-56985-6Published: 05 July 2018

  • eBook ISBN: 978-3-662-49140-9Published: 14 November 2016

  • Series ISSN: 1949-2448

  • Series E-ISSN: 1949-2456

  • Edition Number: 1

  • Number of Pages: X, 244

  • Number of Illustrations: 20 b/w illustrations, 20 illustrations in colour

  • Topics: Microbiology, Biochemical Engineering

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