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Hydrocarbon and Lipid Microbiology Protocols

Biochemical Methods

  • Book
  • © 2016

Overview

Editors:
  1. Terry J. McGenity

    1. School of Biological Sciences, University of Essex, Colchester, Essex, United Kingdom

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  2. Kenneth N. Timmis

    1. Institute of Microbiology, Technical University Braunschweig, Braunschweig, Germany

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  3. Balbina Nogales

    1. Dept. Biology, University of the Balearic Islands and University of the Balearic Islands and Mediterranean Institute for Advanced Studies (IMEDEA, UIB-CSIC)", Palma de Mallorca, Spain

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  • Offers readily-reproducible step-by-step laboratory methods
  • Provides helpful tips and tricks supporting the protocols
  • Includes troubleshooting advice
  • Contains practical laboratory guidance to promote successful results

Part of the book series: Springer Protocols Handbooks (SPH)

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Table of contents (13 protocols)

  1. Front Matter

    Pages i-x
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  2. Introduction

    • Hermann J. Heipieper
    Pages 1-2

  3. Protocols for the Isolation and Analysis of Lipopeptides and Bioemulsifiers

    • Thomas J. Smyth, Michelle Rudden, Konstantina Tsaousi, Roger Marchant, Ibrahim M. Banat
    Pages 3-28

  4. Protocols for the Detection and Chemical Characterisation of Microbial Glycolipids

    • Thomas J. P. Smyth, Michelle Rudden, Konstantina Tsaousi, Roger Marchant, Ibrahim M. Banat
    Pages 29-60

  5. Isolation of Glycoprotein Bioemulsifiers Produced by Marine Bacteria

    • Tony Gutierrez, Ibrahim M. Banat
    Pages 61-74

  6. Protocols on Lipid Extraction from Wet Algal Biomass

    • Forough Ghasemi Naghdi, Peer M. Schenk
    Pages 75-79

  7. Isolation and Characterization of Lipid Droplets from Yeast

    • Karin Athenstaedt
    Pages 81-91

  8. Protocols for Isolation and Analysis of Polyhydroxyalkanoates

    • Mònica Bassas-Galià
    Pages 93-111

  9. Purification of Lipid Rafts from Bacterial Membranes

    • Charlotte Wermser, Daniel Lopez
    Pages 113-122

  10. Isolation and Analysis of Membrane Phospholipids as Biomarkers

    • Nancy Hachicho, Hermann J. Heipieper
    Pages 123-132

  11. Protocols for Purifying and Characterizing Integral Membrane AlkB Enzymes

    • Rachel Narehood Austin, David Born, Thomas J. Lawton, Grace E. Hamilton
    Pages 133-147

  12. Protocols for Structural and Functional Analysis of Particulate Methane Monooxygenase from Methylocystis Species Strain Rockwell (ATCC 49242)

    • Sarah Sirajuddin, Amy C. Rosenzweig
    Pages 149-160

  13. Phenotype-Based Identification of Key Enzymes for Polycyclic Aromatic Hydrocarbon (PAH) Metabolism from Mycobacteria Using Transposon Mutagenesis and a PAH Spray Plate

    • Seong-Jae Kim, Ohgew Kweon, Carl E. Cerniglia
    Pages 161-175

  14. Microcalorimetry as a General Technique to Characterize Ligand Binding

    • Tino Krell, Miriam Rico-Jiménez, Andrés Corral Lugo, José Antonio Reyes Darias, Álvaro Ortega, Abdelali Daddaoua
    Pages 177-188
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Keywords

About this book

This Volume provides protocols for the biochemical analysis of hydrocarbon- and lipid-relevant products, cell components and activities of microbes that interact with hydrophobic compounds. They include methods for the extraction, purification and characterisation of surface tension-reducing bioemulsifiers and biosurfactants that increase the surface area and hence bioavailability of hydrophobic substrates. Protocols for the isolation and biochemical analysis of lipids and polyhydroxyalkanoates, food storage products made during nutrient abundance that represent important biotechnological products, are presented. The extraction of membrane lipid rafts, sub-organelles that fulfil important functional roles for the cell membrane, and the isolation and characterisation of membrane phospholipid biomarkers, are also described. The purification and characterisation of integral membrane hydrocarbon-oxidising enzymes are addressed. Lastly, two generic methods for the genetic analysis of catabolic pathways and analysis of ligand binding are presented.
Hydrocarbon and Lipid Microbiology Protocols
There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.







Editors and Affiliations

  • School of Biological Sciences, University of Essex, Colchester, Essex, United Kingdom

    Terry J. McGenity

  • Institute of Microbiology, Technical University Braunschweig, Braunschweig, Germany

    Kenneth N. Timmis

  • Dept. Biology, University of the Balearic Islands and University of the Balearic Islands and Mediterranean Institute for Advanced Studies (IMEDEA, UIB-CSIC)", Palma de Mallorca, Spain

    Balbina Nogales

Bibliographic Information

  • Book Title: Hydrocarbon and Lipid Microbiology Protocols

  • Book Subtitle: Biochemical Methods

  • Editors: Terry J. McGenity, Kenneth N. Timmis, Balbina Nogales

  • Series Title: Springer Protocols Handbooks

  • DOI: https://doi.org/10.1007/978-3-662-49137-9

  • Publisher: Springer Berlin, Heidelberg

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer-Verlag Berlin Heidelberg 2016

  • Hardcover ISBN: 978-3-662-49135-5Published: 07 September 2016

  • Softcover ISBN: 978-3-662-56984-9Published: 15 June 2018

  • eBook ISBN: 978-3-662-49137-9Published: 26 August 2016

  • Series ISSN: 1949-2448

  • Series E-ISSN: 1949-2456

  • Edition Number: 1

  • Number of Pages: X, 188

  • Number of Illustrations: 19 b/w illustrations, 10 illustrations in colour

  • Topics: Microbiology, Biochemical Engineering

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