Skip to main content
SpringerLink
Log in
Book cover

Protein Sequence Determination

A Sourcebook of Methods and Techniques

  • Book
  • © 1970

Overview

Editors:
  1. Saul B. Needleman

    1. Department of Biochemistry, Northwestern University Medical School and Radioisotope Service V. A. Research Hospital, Chicago, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 74.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (10 chapters)

  1. Front Matter

    Pages I-XXI
    Download chapter PDF

  2. General Considerations

    • Saul B. Needleman
    Pages 1-3

  3. Physical Characterization of the Protein Molecule

    • Ken E. van Holde
    Pages 4-24

  4. End Group Determination

    • Kozo Narita
    Pages 25-90

  5. Amino Acid Composition Determination

    • John W. Eveleigh, George D. Winter
    Pages 91-123

  6. Gas Chromatography of Amino Acid Derivatives

    • Amos Linenberg
    Pages 124-136

  7. Fragmentation of Proteins for Sequence Studies and Separation of Peptide Mixtures

    • Charles B. Kasper
    Pages 137-184

  8. Identification of Specific Amino Acid Residues

    • Ernesto Scoffone, Angelo Fontana
    Pages 185-210

  9. Sequence Determination

    • Pehr Edman
    Pages 211-255

  10. Reconstruction of the Primary Sequence of a Protein from Peptides of Known Sequence

    • Alfred D. Goldstone, Saul B. Needleman
    Pages 256-275

  11. Peptide Synthesis

    • Duane Gish
    Pages 276-310

  12. Back Matter

    Pages 311-348
    Download chapter PDF
Back to top

Keywords

About this book

"All the King's horses and all the King's mm couldn't put Humpty Dumpty together again." It is entirely possible that the difficulty facing "all the King's men" was principally the lack of a sufficient guide to the techniques of reassembling from a series of small components, the original entity. It is the sincere hope of the editor of the present work and of each of the contributing authors that the modern researcher will not face a similar predicament in his endeavours to reconstruct the complete primary sequence of a protein from the array of component amino acids. Rather, it is the intent that, with this volume, he may proceed untimorously if not with outright confidence toward achieving his purpose. To the newcomer in protein sequencing, compelled by necessity, or fascination, to investigate the exact order of amino acids in proteins, the question of "where to begin" - or "how to do it" is urgent. To those more skilled, a ready source of additional techniques should nevertheless be of value. This volume attempts to present in a single source a discussion of the methods and techniques useful to the determination of the primary structures of proteins and peptides. Hopefully, this book will tell the reader "how to do it".

Editors and Affiliations

  • Department of Biochemistry, Northwestern University Medical School and Radioisotope Service V. A. Research Hospital, Chicago, USA

    Saul B. Needleman

Bibliographic Information

Publish with us

Policies and ethics

Back to top

Search

Navigation