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Laboratory Techniques in Membrane Biophysics

An Introductory Course

  • Book
  • © 1969

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Authors:
  1. W. McD. Armstrong

    1. Department of Physiology, Indiana University, Indianapolis, USA

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  2. K. Baumann

    1. Physiologisches Institut, Freien Universität, Berlin, Germany

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  3. P. C. Caldwell

    1. Department of Zoology, University of Bristol, Great Britain

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  4. T. W. Clarkson

    1. Department of Radiation Biology and Biophysics, University of Rochester, Rochester, USA

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  5. J. Dudel

    1. II. Physiologisches Institut, Universität Heidelberg, Heidelberg, Germany

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  6. B. Frankenhaeuser

    1. Nobel Institute for Neurophysiology, Karolinska Institutet, Stockholm, Sweden

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  7. E. Frömter

    1. Physiologisches Institut, Freien Universität, Berlin, Germany

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  8. G. Gardos

    1. Department of Cell Metabolism, Research Institute of the National Blood Center, Budapest, Hungary

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  9. B. Z. Gilzburg

    1. Department of Botany, Hebrew University, Jerusalem, Israel

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  10. J. F. Hoffman

    1. Department of Physiology, Yale University, New Haven, USA

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  11. A. Kepes

    1. Laboratoire de Biologie Moléculaire, Collège de France, Paris, France

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  12. R. D. Keynes

    1. Institute of Animal Physiology, Agricultural Research Council, Babraham, Cambridge, Great Britain

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  13. A. Kleinzeller

    1. Department of Physiology, University of Pennsylvania, Philadelphia, USA

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  14. P. G. Kostyuk

    1. Institute of Physiology, Kiev, USSR

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  15. A. Kotyk

    1. Institute of Microbiology, Czechoslovak Academy of Sciences, Prague, Czechoslovakia

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  16. A. A. Lev

    1. Institute of Cytology, Academy of Sciences of USSR, Leningrad, USSR

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  17. B. Lindemann

    1. II. Physiologisches Institut, Universität des Saarlandes, Homburg a. d. Saar, Germany

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  18. P. Mueller

    1. Eastern Pennsylvania Psychiatric Institute, Philadelphia, USA

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  19. H. Muth

    1. Institut für Biophysik, Universität des Saarlandes, Homburg a. d. Saar, Germany

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  20. W. Nonner

    1. I. Physiologisches Institut, Universität des Saarlandes, Homburg a. d. Saar, Germany

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  21. E. Oberhausen

    1. Institut für Biophysik, Universität des Saarlandes, Homburg a. d. Saar, Germany

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  22. H. Passow

    1. II. Physiologisches Institut, Universität des Saarlandes, Homburg a. d. Saar, Germany

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  23. A. Rothstein

    1. Department of Radiation Biology and Biophysics, University of Rochester, Rochester, USA

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  24. D. O. Rudin

    1. Department of Basic Research, Eastern Pennsylvania Psychiatric Institute, Philadelphia, USA

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  25. R. Stämpfli

    1. I. Physiologisches Institut, Universität des Saarlandes, Homburg a. d. Saar, Germany

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  26. T. Teorell

    1. Department of Physiology, University of Uppsala, Uppsala, Sweden

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  27. K. J. Ullrich

    1. Physiologisches Institut, Freien Universität, Berlin, Germany

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  28. N. A. Walker

    1. School of Biological Sciences, University of Sydney, Sydney, Australia

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  29. W. Wilbrandt

    1. Pharmakologisches Institut, Universität Bern, Bern, Switzerland

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Editors:
  1. H. Passow

    1. II. Physiologisches Institut, Universität des Saarlandes, Homburg a. d. Saar, Germany

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  2. R. Stämpfli

    1. I. Physiologisches Institut, Universität des Saarlandes, Homburg a. d. Saar, Germany

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Table of contents (16 chapters)

  1. Front Matter

    Pages I-VIII
    Download chapter PDF

  2. Measurement of Hydraulic Conductivity and Reflexion Coefficients of a Plant Cell Membrane by means of Transcellular Osmosis

    • B. Z. Ginzburg, N. A. Walker
    Pages 1-8

  3. Flux Measurements in Erythrocytes

    • G. Gardos, J. F. Hoffman, H. Passow
    Pages 9-20

  4. Ion Permeability of Erythrocyte Ghosts

    • H. Passow
    Pages 21-27

  5. Countertransport in Human Red Blood Cells

    • W. Wilbrandt
    Pages 28-33

  6. Kinetics of Cation Transport in Yeast

    • W. McD. Armstrong, A. Rothstein
    Pages 34-48

  7. Bacterial Permeases

    • A. Kepes
    Pages 49-62

  8. The Exchange of 22Na between Frog Sartorius Muscle and the Bathing Medium

    • P. C. Caldwell, R. D. Keynes
    Pages 63-68

  9. Determination of Intracellular Ionic Concentrations and Activities

    • A. Kleinzeller, P. G. Kostyuk, A. Kotyk, A. A. Lev
    Pages 69-84

  10. Experiments on Na Transport of Frog Skin Epithelium

    • T. W. Clarkson, B. Lindemann
    Pages 85-105

  11. Micropuncture and Microanalysis in Kidney Physiology

    • K. J. Ullrich, E. Frömter, K. Baumann
    Pages 106-129

  12. Oscillatory Phenomena in a Porous, Fixed Charge Membrane

    • T. Teorell
    Pages 130-140

  13. Bimolecular Lipid Membranes: Techniques of Formation, Study of Electrical Properties, and Induction of Ionic Gating Phenomena

    • P. Mueller, D. O. Rudin
    Pages 141-156

  14. Dissection of Single Nerve Fibres and Measurement of Membrane Potential Changes of Ranvier Nodes by means of the Double Air Gap Method

    • R. Stämpfli
    Pages 157-166

  15. Ionic Currents in the Myelinated Nerve Fibre

    • B. Frankenhaeuser
    Pages 167-170

  16. A New Voltage Clamp Method

    • W. Nonner, R. Stämpfli
    Pages 171-175

  17. Electrical Activity of Synapses. The Crayfish Neuromuscular Junction

    • J. Dudel
    Pages 176-180

  18. Back Matter

    Pages 181-201
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Keywords

About this book

The present manual contains a collection of laboratory instructions used during an international training course on membrane biophysics which was held at Homburg in the fall of 1966. The selection of the topics dealt with in the various chapters depended on the scientific interest of the available teachers and on the availability of the necessary equipment in our laboratories. Thus, the material included in this volume does not add up to a systematic course in membrane biophysics. Instead it represents a more fortuitous collection of laboratory problems. In addition, some authors place more emphasis on teaching the more technical aspects of a method whereas others are primarily concerned with the demonstra­ tion of a significant biological phenomenon. Nevertheless, in spite of such differences of emphasis and a somewhat haphazard choice of a few methods and phenomena among many others of similar importance, it was felt that the publication of the material is desirable. Since no other laboratory manual exists so far, the present laboratory problems which were tested in actual practice may serve as a useful basis for the shaping of further training courses or for laboratory courses for graduate students in biophysics and physiology. Our thanks are due to the authors and the publisher who were patient and kind enough to cooperate with the editors during the long period between the end of the course and the appearance of the book.

Authors, Editors and Affiliations

  • II. Physiologisches Institut, Universität des Saarlandes, Homburg a. d. Saar, Germany

    H. Passow, B. Lindemann, H. Passow

  • I. Physiologisches Institut, Universität des Saarlandes, Homburg a. d. Saar, Germany

    R. Stämpfli, W. Nonner, R. Stämpfli

  • Department of Physiology, Indiana University, Indianapolis, USA

    W. McD. Armstrong

  • Physiologisches Institut, Freien Universität, Berlin, Germany

    K. Baumann, E. Frömter, K. J. Ullrich

  • Department of Zoology, University of Bristol, Great Britain

    P. C. Caldwell

  • Department of Radiation Biology and Biophysics, University of Rochester, Rochester, USA

    T. W. Clarkson, A. Rothstein

  • II. Physiologisches Institut, Universität Heidelberg, Heidelberg, Germany

    J. Dudel

  • Nobel Institute for Neurophysiology, Karolinska Institutet, Stockholm, Sweden

    B. Frankenhaeuser

  • Department of Cell Metabolism, Research Institute of the National Blood Center, Budapest, Hungary

    G. Gardos

  • Department of Botany, Hebrew University, Jerusalem, Israel

    B. Z. Gilzburg

  • Department of Physiology, Yale University, New Haven, USA

    J. F. Hoffman

  • Laboratoire de Biologie Moléculaire, Collège de France, Paris, France

    A. Kepes

  • Institute of Animal Physiology, Agricultural Research Council, Babraham, Cambridge, Great Britain

    R. D. Keynes

  • Department of Physiology, University of Pennsylvania, Philadelphia, USA

    A. Kleinzeller

  • Institute of Physiology, Kiev, USSR

    P. G. Kostyuk

  • Institute of Microbiology, Czechoslovak Academy of Sciences, Prague, Czechoslovakia

    A. Kotyk

  • Institute of Cytology, Academy of Sciences of USSR, Leningrad, USSR

    A. A. Lev

  • Eastern Pennsylvania Psychiatric Institute, Philadelphia, USA

    P. Mueller

  • Institut für Biophysik, Universität des Saarlandes, Homburg a. d. Saar, Germany

    H. Muth, E. Oberhausen

  • Department of Basic Research, Eastern Pennsylvania Psychiatric Institute, Philadelphia, USA

    D. O. Rudin

  • Department of Physiology, University of Uppsala, Uppsala, Sweden

    T. Teorell

  • School of Biological Sciences, University of Sydney, Sydney, Australia

    N. A. Walker

  • Pharmakologisches Institut, Universität Bern, Bern, Switzerland

    W. Wilbrandt

Bibliographic Information

  • Book Title: Laboratory Techniques in Membrane Biophysics

  • Book Subtitle: An Introductory Course

  • Authors: W. McD. Armstrong, K. Baumann, P. C. Caldwell, T. W. Clarkson, J. Dudel, B. Frankenhaeuser, E. Frömter, G. Gardos, B. Z. Gilzburg, J. F. Hoffman, A. Kepes, R. D. Keynes, A. Kleinzeller, P. G. Kostyuk, A. Kotyk, A. A. Lev, B. Lindemann, P. Mueller, H. Muth, W. Nonner, E. Oberhausen, H. Passow, A. Rothstein, D. O. Rudin, R. Stämpfli, T. Teorell, K. J. Ullrich, N. A. Walker, … W. Wilbrandt

  • Editors: H. Passow, R. Stämpfli

  • DOI: https://doi.org/10.1007/978-3-642-87259-4

  • Publisher: Springer Berlin, Heidelberg

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer-Verlag Berlin · Heidelberg 1969

  • Softcover ISBN: 978-3-540-04592-2Published: 01 January 1969

  • eBook ISBN: 978-3-642-87259-4Published: 06 December 2012

  • Edition Number: 1

  • Number of Pages: VIII, 201

  • Topics: Human Physiology, Biochemistry, general, Biological and Medical Physics, Biophysics

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