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Intracellular Staining in Neurobiology

  • Book
  • © 1973

Overview

Editors:
  1. Stanley B. Kater

    1. Department of Zoology, University of Iowa, Iowa City, USA

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  2. Charles Nicholson

    1. Division of Neurobiology, Department of Physiology and Biophysics, University of Iowa, Iowa City, USA

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    You can also search for this editor in PubMed   Google Scholar

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Table of contents (20 chapters)

  1. Front Matter

    Pages i-xiii
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  2. The Development of Intracellular Staining

    1. The Development of Intracellular Staining

      • Charles Nicholson, Stanley B. Kater
      Pages 1-19

  4. Recent Applications of Intracellular Staining

    1. Microelectrode Injection, Axonal Iontophoresis, and the Structure of Neurons

      • Brian Mulloney
      Pages 99-113

    2. Permeability and Structure of Electrotonic Junctions and Intercellular Movements of Tracers

      • M. V. L. Bennett
      Pages 115-134

    3. Characterization of an Insect Neuron which cannot be Visualized in situ

      • R. K. Murphey
      Pages 135-150

    4. Intracellular Staining Techniques in Gastropod Molluscs

      • C. R. S. Kaneko, S. B. Kater
      Pages 151-156

    5. Morphological Identification of Single Cells in the Fish Retina by Intracellular Dye Injection

      • Akimichi Kaneko
      Pages 157-179

    6. Morphological and Physiological Identification of Retinal Cells in the Turtle

      • Yoko Hashimoto, Takehiko Saito, William H. Miller, Tsuneo Tomita
      Pages 181-188

    7. Morphological Identification of Simple, Complex and Hypercomplex Cells in the Visual Cortex of the Cat

      • David Van Essen, James Kelly
      Pages 189-198

    8. Procion Yellow Staining of Functionally Identified Interneurons in the Spinal Cord of the Cat

      • E. Jankowska, S. Lindström
      Pages 199-209

    9. Procion Yellow and Cobalt as Tools for the Study of Structure-Function Relationships in Vertebrate Central Nervous Systems

      • Rodolfo Llinás
      Pages 211-225

  5. Quantitative Analyses

    1. Computer Analysis of Neuronal Structure

      • D. R. Reddy, W. J. Davis, R. B. Ohlander, D. J. Bihary
      Pages 227-253

    2. The Use of Intracellular Dye Injections in the Study of Small Neural Networks

      • Allen I. Selverston
      Pages 255-280

    3. Determination of Neuronal Membrane Properties Using Intracellular Staining Techniques

      • John N. Barrett
      Pages 281-299

  6. Practical Considerations

    1. Current Practice and Prospects for the Intracellular Stains

      • Donald Kennedy
      Pages 301-306
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Keywords

About this book

The purpose of this book is to provide basic reference material to facilitate the further development and application of the intracellular staining techniques which originated with the introduction of Procion dye as a neurocellular stain. We had sev­ eral specific objectives in compiling these chapters. First, we recognized that both the techniques and results of intracellular staining have been derived from a variety of preparations and published in diverse journals. Consequently, we tried to make this information more readily available by incorporating several reviews by original authors. This should provide the reader with a knowledge of the kinds of neurobiological prob­ lems for which intracellular staining has already been employed. A second objective was to facilitate extension of these methods to a wider variety of problems. To this end there are chapters dealing with Procion dye as a marker for ultrastructural investi­ gations, the use of cobalt as a staining compound and the implementation of computer techniques for quantitative studies of neuronal relationships. As background for further extension of these methods this volume contains material on the history of the discovery of Procion dyes as intracellular stains, the chemistry of these dyes, and fundamentals of fluorescence microscopy. Our final specific objective was to present details of technical procedures that were not readily available in the literature.

Editors and Affiliations

  • Department of Zoology, University of Iowa, Iowa City, USA

    Stanley B. Kater

  • Division of Neurobiology, Department of Physiology and Biophysics, University of Iowa, Iowa City, USA

    Charles Nicholson

Bibliographic Information

  • Book Title: Intracellular Staining in Neurobiology

  • Editors: Stanley B. Kater, Charles Nicholson

  • DOI: https://doi.org/10.1007/978-3-642-87123-8

  • Publisher: Springer Berlin, Heidelberg

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer-Verlag Berlin Heidelberg 1973

  • Softcover ISBN: 978-3-642-87125-2Published: 23 August 2014

  • eBook ISBN: 978-3-642-87123-8Published: 06 December 2012

  • Edition Number: 1

  • Number of Pages: XIII, 332

  • Number of Illustrations: 158 b/w illustrations, 32 illustrations in colour

  • Topics: Neurobiology, Neurosciences, Biological Techniques

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