Molecular Techniques in Taxonomy

1. Front Matter

   Pages I-X

   PDF

2. A Prologue

   1. Past and Future of Taxonomy

      • Jean R David

      Pages 1-3

3. Overviews

   1. Taxonomy: An Essential Key to Evolutionary Biology

      • R J Berry

      Pages 5-13

   2. Variation at the DNA Level: Something for Everyone

      • Martin Kreitman

      Pages 15-32

   3. Molecular Systematics at the Species Boundary: Exploiting Conserved and Variable Regions of the Mitochondrial Genome of Animals via Direct Sequencing from Amplified DNA

      • Chris Simon

      Pages 33-71

   4. Ribosomal RNA Phylogenies

      • M. Solignac, M. Pélandakis, F. Rousset, A. Chenuil

      Pages 73-85

   5. Evaluating Gene Versus Genome Evolution

      • Robert Cedergren, Yvon Abel, David Sankoff

      Pages 87-99

   6. DNA and Higher Plant Systematics: Some Examples from the Legumes

      • Jeff J. Doyle, Jane L. Doyle

      Pages 101-115

   7. DNA-DNA Hybridization: Principles and Results

      • Jeffrey R. Powell, Adalgisa Caccone

      Pages 117-132

   8. Satellite DNA

      • L. Bachmann, M. Raab, J. Schibel, D. Sperlich

      Pages 133-143

   9. DNA Fingerprinting

      • David T Parkin, J H Wetton

      Pages 145-157

   10. The Statistical Interpretation of Hypervariable DNAs

       • J. F. Y. Brookfield

       Pages 159-169

4. Case Studies

   1. A Multidimensional Approach to the Evolution and Systematics of Dolichopoda Cave Crickets

      • V. Sbordoni, G. Allegrucci, D. Cesaroni

      Pages 171-199

   2. Sperm and Evolution in Drosophila: morphological and molecular aspects

      • D. Lachaise, D. Joly

      Pages 201-216

   3. Episodic Evolutionary Change in Local Populations

      • H. L. Carson

      Pages 217-232

   4. Searching for Speciation Genes in the Species Pair Drosophila mojavensis and D. arizonae

      • E. Zouros

      Pages 233-247

   5. Colonizing Species of Drosophila

      • Antonio Fontdevila

      Pages 249-269

   6. Molecular Taxonomy in the Control of West African Onchocerciasis

      • R. J. Post, K. A. Murray, P. Flook, A. L. Millest

      Pages 271-281

5. Protocols

   1. DNA Protocols for Plants

      • Jeffrey Doyle

      Pages 283-293

   2. Preparation and Visualization of Mitochondrial DNA for RFLP Analysis

      • Michel Solignac

      Pages 295-319

Taxonomy is fundamental to understanding the variety of life forms, and exciting expansions in molecular biology are re- volutionising the obtained data. This volume reviews the ma- jor molecular biological techniques that are applied in ta- xonomy. The chapters are arranged in three main sections:1) Overviews of important topics in molecular taxonomy; 2) Case studies of the successful application of molecular methods to taxonomic and evolutionary questions; 3) Protocols for a range of generally applicable methods. The described techni- ques include DNA-DNA hybridization, DNA fingerprinting, RFLP analysis, and PCR sequencing.

• DNA
• PCR
• Phylogenie
• biology
• evolution
• evolutionary biology
• gene
• genetics
• molecular aspects
• molecular biology
• plant
• plant systematics
• plants
• systematics
• taxonomy

• School of Biological Sciences, University of East Anglia, Norwich, UK

  Godfrey M. Hewitt, Andrew W. B. Johnston

• John Innes Institute, Norwich, UK

  J. Peter W. Young

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