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Plant Cell and Tissue Culture

A Laboratory Manual

  • Book
  • © 1982

Overview

Authors:
  1. Jakob Reinert

    1. Institut für Pflanzenphysiologie und Zellbiologie, Berlin 33, Federal Republic of Germany

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  2. Michael Magson Yeoman

    1. Department of Botany, University of Edinburgh, Edinburgh, Great Britain

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    You can also search for this author in PubMed   Google Scholar

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Table of contents (19 chapters)

  1. Front Matter

    Pages i-v
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  2. Introduction

    1. Introduction

      • Jakob Reinert, Michael Magson Yeoman
      Pages 1-1

  3. Isolation of Plant Material and Studies on Growth and Cell Division

    1. Front Matter

      Pages 3-3
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    2. Isolation of Explants, Establishment and Maintenance of Callus (Daucus carota)

      • Jakob Reinert, Michael Magson Yeoman
      Pages 4-7

    3. Growth and Cell Division in Cultured Artichoke Explants

      • Jakob Reinert, Michael Magson Yeoman
      Pages 8-10

    4. Initiation and Establishment of Cell Suspension Cultures of Carrot (Daucus carota)

      • Jakob Reinert, Michael Magson Yeoman
      Pages 11-13

    5. Isolation and Culture of Single Cells and Examination of the ‘Conditioning’ Effect

      • Jakob Reinert, Michael Magson Yeoman
      Pages 14-17

  4. Bioassay Systems for Cytokinins

    1. Front Matter

      Pages 19-19
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    2. Soybean Bioassay System

      • Jakob Reinert, Michael Magson Yeoman
      Pages 20-21

    3. Tobacco Callus Bioassay System

      • Jakob Reinert, Michael Magson Yeoman
      Pages 22-23

  5. Morphogenesis in Vitro: Studies on Regeneration

    1. Front Matter

      Pages 25-25
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    2. Embryogenesis in Cultured Cells of Carrot

      • Jakob Reinert, Michael Magson Yeoman
      Pages 26-28

    3. The Tobacco Pith System

      • Jakob Reinert, Michael Magson Yeoman
      Pages 29-31

    4. Culture of Anthers from Nicotiana tabacum and the Establishment of Haploid Plants from Embryos

      • Jakob Reinert, Michael Magson Yeoman
      Pages 32-34

    5. Vegetative Propagation of Orchids (Cymbidium)

      • Jakob Reinert, Michael Magson Yeoman
      Pages 35-38

  6. Isolation, Culture and Fusion of Protoplasts from Higher Plants

    1. Front Matter

      Pages 39-39
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    2. Isolation and Culture of Mesophyll Protoplasts from Tobacco Leaves

      • Jakob Reinert, Michael Magson Yeoman
      Pages 40-43

    3. Protoplast Fusion Induced by Polyethylene Glycol (PEG)

      • Jakob Reinert, Michael Magson Yeoman
      Pages 44-45

  7. Secondary Metabolites in Tissue Cultures

    1. Front Matter

      Pages 47-47
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    2. Callus Formation and Anthocyanin Production in Cultures of Haplopappus gracilis

      • Jakob Reinert, Michael Magson Yeoman
      Pages 48-50
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Keywords

About this book

The techniques of plant organ, tissue, and cell culture concentrated on reproducibility, simplicity and accu­ are now established in many research laboratories racy with sufficient illustration to make all mani­ throughout the world and are being used in numerous pulations clear. areas of plant science. Methods have been developed The drawings of items used in the bench layout to propagate plants and free them from viruses using diagrams are symbolic and are 'keyed in' by number to shoot tip culture. The regeneration of plants from callus the list of materials and equipment. A line around an culture has also proved useful commercially. Elegant item indicates that is sterile. techniques have been used to synthesise somatic The adoption of an integrated text in which diagrams hybrids by the fusion of protoplasts and to transform are related spatially to the methods will, we hope, help cells. These and many other techniques have been the student to grasp the techniques quickly and effec­ and can be used to investigate a variety of botanical tively. This is first and foremost a manual which has its phenomena as well as to improve crop plants and now place on the laboratory bench open in front of the provide an important part of the basic experimental student, a book to be used! skills required by a majority of experimental botanists.

Authors and Affiliations

  • Institut für Pflanzenphysiologie und Zellbiologie, Berlin 33, Federal Republic of Germany

    Jakob Reinert

  • Department of Botany, University of Edinburgh, Edinburgh, Great Britain

    Michael Magson Yeoman

Bibliographic Information

  • Book Title: Plant Cell and Tissue Culture

  • Book Subtitle: A Laboratory Manual

  • Authors: Jakob Reinert, Michael Magson Yeoman

  • DOI: https://doi.org/10.1007/978-3-642-81784-7

  • Publisher: Springer Berlin, Heidelberg

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer-Verlag Berlin Heidelberg 1982

  • Softcover ISBN: 978-3-642-81786-1Published: 05 January 2012

  • eBook ISBN: 978-3-642-81784-7Published: 06 December 2012

  • Edition Number: 1

  • Number of Pages: V, 83

  • Topics: Cell Biology

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