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Membrane Spectroscopy

  • Book
  • © 1981

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Editors:
  1. Ernst Grell

    1. Max-Planck-Institut für Biophysik, Frankfurt 71, Germany

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Table of contents (9 chapters)

  1. Front Matter

    Pages I-XI
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  2. Nuclear Magnetic Resonance Studies of the Phospholipid Bilayer Membrane

    • S. I. Chan, D. F. Bocian, N. O. Petersen
    Pages 1-50

  3. Electron Spin Resonance: Spin Labels

    • D. Marsh
    Pages 51-142

  4. Absorption and Circular Dichroism Spectroscopies

    • M. M. Long, D. W. Urry
    Pages 143-171

  5. Optical Spectroscopy of Monolayers, Multilayer Assemblies, and Single Model Membranes

    • J. Heesemann, H. P. Zingsheim
    Pages 172-198

  6. Fluorescence Spectroscopy of Biological Membranes

    • J. Yguerabide, M. C. Foster
    Pages 199-269

  7. Infrared Membrane Spectroscopy

    • U. P. Fringeli, Hs. H. Günthard
    Pages 270-332

  8. Chemical Relaxation Spectrometry

    • H. Ruf, E. Grell
    Pages 333-376

  9. Raman Spectroscopy of Membrane Constituents and Related Molecules

    • R. C. Lord, R. Mendelsohn
    Pages 377-436

  10. Low-Angle X-Ray Diffraction

    • N. P. Franks, Y. K. Levine
    Pages 437-487

  11. Back Matter

    Pages 488-500
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Keywords

About this book

The last 10 years have seen an enormous growth in our understanding of the molecular organisation of biological membranes. Experimental methods have been devised to meas­ ure the translational and rotational mobility of lipids and proteins, thereby furnishing a quantitative basis for the concept of membrane fluidity. Likewise, the asymmetry of bi­ layer membranes as evidenced by the asymmetric insertion of proteins and lipids has been put on firm experimental ground. At higher molecular resolution it has been possible to provide a detailed pi2ture of the molecular conformation and dynamics of lipids and, to some extent, even of small peptides embedded in a bilayer matrix. Many of these achieve­ ments would not have been possible without the application of modem spectroscopic methods. Since these techniques are scattered in a variety of specialized textbooks the present monograph attempts to describe the key spectroscopic methods employed in present-day membrane research at an intermediate level. There is no question that the elusive detailed structure of the biological membrane demands a multiplicity of experi­ mental approaches and that no single spectroscopic method can cover the full range of physical phenomena encountered in a membrane. Much confusion in the literature has arisen by undue generalizations without considering the frequency range or other limi­ tations of the methods employed. It is to be hoped that the present monograph with its comprehensive description of most modem spectroscopic techniques, will contribute to- .

Editors and Affiliations

  • Max-Planck-Institut für Biophysik, Frankfurt 71, Germany

    Ernst Grell

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