Protein Sequence Determination

1. Front Matter

   Pages I-XVIII

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2. General Considerations

   • Saul B. Needleman

   Pages 1-4

3. Physical Characterization of the Protein Molecule

   • Ken E. Van Holde

   Pages 5-29

4. End Group Determination

   • Kozo Narita, Hisayuki Matsuo, Terumi Nakajima

   Pages 30-103

5. Improved Tritium-Labeling for Quantitative C-Terminal Analysis

   • Hisayuki Matsuo, Kozo Narita

   Pages 104-113

6. Fragmentation of Proteins for Sequence Studies and Separation of Peptide Mixtures

   • Charles B. Kasper

   Pages 114-161

7. Identification of Specific Amino Acid Residues

   • Ernesto Scoffone, Angelo Fontana

   Pages 162-203

8. Amino Acid Composition by Column Chromatography

   • P. E. Hare

   Pages 204-231

9. Sequence Determination

   • Pehr Edman, Agnes Henschen

   Pages 232-279

10. Analysis of Amino Acid Phenylthiohydantoins by Gas Chromatography and High Performance Liquid Chromatography

    • John J. Pisano

    Pages 280-297

11. Reconstruction of the Primary Sequence of a Protein from Peptides of Known Sequence

    • Alfred D. Goldstone, Saul B. Needleman

    Pages 298-321

12. Peptide Synthesis

    • Duane Gish

    Pages 322-356

13. Back Matter

    Pages 357-396

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In the few years since the first edition of this book made its appearance, under­ takings in the area of protein sequence determination have increased at an almost logarithmic pace. The logic governing such efforts has, predictably, retreated from a simple sense of curiosity in achieving something that had not previously been done, to be replaced by an absolute requirement for sequence information as the best means for understanding heretofore unattainable aspects of chemical, and structural and functional mechanistic phenomena. Witness, for example, recent volumes of the Annual Review of Biochemistry and other review journals, which treat the genetic control of mitochondrial proteins, mechanisms of enzyme action, the immunochemistry of collagen, and the role of lysosomal enzymes in the lipid storage diseases - all on the basis of known protein sequence information. One must note, too, the appearance of related works in the area of nucleic acid sequencing. The technologic advances in chemical sequencing procedures have proliferated at a remarkable rate. It is in the area of instrumentation, however, that the greatest advances have taken place. As a consequence of this, each chapter (with one single exception) in this book has been extensively revised and updated. Entire chapters have been replaced in several cases, without minimizing the value of their original content. In addition, a second volume will appear soon, dealing with subjects not covered in the present book.

• Peptide
• Sedimentation
• Termination
• amino acid
• biochemistry
• chemistry
• enzyme
• enzymes
• instrumentation
• mitochondria
• molecule
• nucleic acid
• peptides
• protein
• proteins

• Scientific Affairs, Diagnostics Division, Abbott Laboratories, North Chicago, USA

  Saul B. Needleman

• Departments of Biochemistry and Neurology, Northwestern University Medical School, Chicago, USA

  Saul B. Needleman

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