Tracer Methods in Hormone Research

1. Front Matter

   Pages I-XI

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2. Introduction

   • Erlio Gurpide

   Pages 1-4

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3. Rates in Open System in the Steady State: Definitions and Relations

   • Erlio Gurpide

   Pages 5-28

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4. Infusion of Tracers at a Constant Rate

   • Erlio Gurpide

   Pages 29-43

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5. Rapid Injection of Tracers

   • Erlio Gurpide

   Pages 44-70

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6. Tracer Kinetics in Compartmental Models

   • Erlio Gurpide

   Pages 71-104

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7. Interpretation of Isotopic Data from Blood — Borne Compounds

   • Erlio Gurpide

   Pages 105-147

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8. Rates of Secretion and Metabolism of Hormones Estimated from Specific Activities of Urinary Metabolites

   • Erlio Gurpide

   Pages 148-164

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9. In vitro Tracer Superfusion Experiments

   • Erlio Gurpide

   Pages 165-175

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10. Back Matter

    Pages 176-190

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The purpose of this monograph is to describe theoretical aspects of the interpretation of data obtained from experiments performed with labeled hormones. Quantitative endocrinologic studies involving the use of tracers include the determination of rates at which hormones are secreted by endocrine glands and are produced outside these glands by conversion of other secreted hor­ mones. Tracer experiments are also performed with the purpose of measuring rates of metabolic reactions. These measurements reveal the contribution of secreted hormones to the formation of circulating compounds and urinary metabolites. The estimation of rates of fetal and placental production and exchange of hormones characterizes a class of in vivo quantitative studies performed with isotopically labeled hormones (radioactive or not). In addi­ tion, tracers are used to measure permeability and rates of reaction in in vitro systems, and to study the uptake of hormones by tissues, both in vivo and in vitro. The stability of the steroid nucleus carrying the isotopic label and the large number of reversible metabolic reactions in which steroids are involved, both facilitated and motivated the development of a sophisticated theoretical treat­ ment of tracer experiments in the field of endocrinology. Although the prac­ tical examples used to illustrate the concepts and calculations presented in this monograph involve labeled hormones, the theory is presented in a general symbolic manner and is applicable to other fields of investigation.

• Termination
• hormone
• steroid
• steroids
• tissue

• Mount Sinai School of Medicine, City University of New York, New York, USA

  Erlio Gurpide

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