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  • Book
  • © 1998

Animal Cell Culture Techniques

Editors:

  • Clearly structured protocols for direct use in the laboratory
  • Step-by step instructions with full practical details and background information
  • Several of the reliable well-tested protocols are contributed by authors from the renowned Irish National Cell and Tissue Culture Centre

Part of the book series: Springer Lab Manuals (SLM)

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Table of contents (33 chapters)

  1. Front Matter

    Pages I-XIII
  2. General Methods

    1. Front Matter

      Pages 1-1
    2. Cloning Animal Cells

      • Shirley McBride, Mary Heenan, Martin Clynes
      Pages 3-12
    3. Cell Culture and Diagnostic Virology

      • Edwin O’Kelly
      Pages 13-37
    4. Serum-Free Media

      • Joanne Keenan, Paula Meleady, Martin Clynes
      Pages 54-66
    5. Screening Culture Media and Sera for Endotoxin Testing

      • Joanne Keenan, Martin Clynes
      Pages 67-81
    6. Generation of Monoclonal Antibodies and Characterization of Novel Antibodies by Western Blotting and Immunocytochemistry

      • Elizabeth Moran, Annemarie Larkin, Allan Masterson, Martin Clynes
      Pages 82-104
    7. Primary Culture

      • Finbar O’Sullivan, Paula Meleady, Shirley McBride, Martin Clynes
      Pages 115-128
  3. Cell proliferation and Death

    1. Front Matter

      Pages 129-129
    2. Methods for Studying Phosphoinositide Metabolism in Cultured Cells

      • Conor Duffy, Ailish Hynes, Leo Quinlan
      Pages 149-169
    3. Analysis of Cell Cycle and Cell Death Mechanisms

      • S. Verhaegen, Seamus Coyle, Lisa M. Connolly, Colette O’Loughlin, Martin Clynes
      Pages 170-193
  4. In Vitro Models for Cell Differentiation

    1. Front Matter

      Pages 195-195
    2. Assessing Release of Secretory Products from Individual Cells

      • Domenico Bosco, Paolo Meda
      Pages 197-216
    3. Investigations into Cell Differentiation Using Cells in Culture

      • Shirley McBride, Martin Clynes
      Pages 231-239
    4. Cell Culture Models for Mononuclear Phagocytes

      • Philippe Poindron, Yves Lombard
      Pages 240-263

About this book

Cell culture techniques allow a variety of molecular and cell biological questions to be addressed, offering physiological conditions whilst avoiding the use of laboratory animals. In addition to basic techniques, a wide range of specialised practical protocols covering the following areas are included: cell proliferation and death, in-vitro models for cell differentiation, in-vitro models for toxicology and pharmacology, industrial application of animal cell culture, genetic manipulation and analysis of human and animal cells in culture.

Editors and Affiliations

  • National Cell & Tissue Culture Center Bioresearch Ireland, Dublin City University, Glasnevin Dublin 9, Ireland

    Martin Clynes

Bibliographic Information

  • Book Title: Animal Cell Culture Techniques

  • Editors: Martin Clynes

  • Series Title: Springer Lab Manuals

  • DOI: https://doi.org/10.1007/978-3-642-80412-0

  • Publisher: Springer Berlin, Heidelberg

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer-Verlag Berlin Heidelberg 1998

  • Softcover ISBN: 978-3-540-63008-1Published: 16 June 1998

  • eBook ISBN: 978-3-642-80412-0Published: 06 December 2012

  • Edition Number: 1

  • Number of Pages: XIII, 618

  • Number of Illustrations: 34 b/w illustrations

  • Topics: Cell Biology, Human Physiology, Biochemistry, general

Buy it now

Buying options

eBook USD 119.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 159.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access