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Animal Cell Culture Techniques

  • Book
  • © 1998

Overview

Editors:
  1. Martin Clynes

    1. National Cell & Tissue Culture Center Bioresearch Ireland, Dublin City University, Glasnevin Dublin 9, Ireland

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  • Clearly structured protocols for direct use in the laboratory
  • Step-by step instructions with full practical details and background information
  • Several of the reliable well-tested protocols are contributed by authors from the renowned Irish National Cell and Tissue Culture Centre

Part of the book series: Springer Lab Manuals (SLM)

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Table of contents (33 chapters)

  1. Front Matter

    Pages I-XIII
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  2. General Methods

    1. Front Matter

      Pages 1-1
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    2. Cloning Animal Cells

      • Shirley McBride, Mary Heenan, Martin Clynes
      Pages 3-12

    3. Cell Culture and Diagnostic Virology

      • Edwin O’Kelly
      Pages 13-37

    4. Screening for Mycoplasma Contamination in Animal Cell Cultures

      • Mary Heenan, Martin Clynes
      Pages 38-53

    5. Serum-Free Media

      • Joanne Keenan, Paula Meleady, Martin Clynes
      Pages 54-66

    6. Screening Culture Media and Sera for Endotoxin Testing

      • Joanne Keenan, Martin Clynes
      Pages 67-81

    7. Generation of Monoclonal Antibodies and Characterization of Novel Antibodies by Western Blotting and Immunocytochemistry

      • Elizabeth Moran, Annemarie Larkin, Allan Masterson, Martin Clynes
      Pages 82-104

    8. The Generation of Long-Term Human Antibody-Producing Cell Lines Using the Epstein-Barr Virus

      • Olivia Flynn, Dermot Walls
      Pages 105-114

    9. Primary Culture

      • Finbar O’Sullivan, Paula Meleady, Shirley McBride, Martin Clynes
      Pages 115-128

  3. Cell proliferation and Death

    1. Front Matter

      Pages 129-129
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    2. Baculoviral Expression and Partial Purification of Cyclin/CDK Complexes

      • Mark Jackman
      Pages 131-148

    3. Methods for Studying Phosphoinositide Metabolism in Cultured Cells

      • Conor Duffy, Ailish Hynes, Leo Quinlan
      Pages 149-169

    4. Analysis of Cell Cycle and Cell Death Mechanisms

      • S. Verhaegen, Seamus Coyle, Lisa M. Connolly, Colette O’Loughlin, Martin Clynes
      Pages 170-193

  4. In Vitro Models for Cell Differentiation

    1. Front Matter

      Pages 195-195
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    2. Assessing Release of Secretory Products from Individual Cells

      • Domenico Bosco, Paolo Meda
      Pages 197-216

    3. The Granulocyte/Macrophage Colony-Forming Unit Assay

      • Augusto Pessina
      Pages 217-230

    4. Investigations into Cell Differentiation Using Cells in Culture

      • Shirley McBride, Martin Clynes
      Pages 231-239

    5. Cell Culture Models for Mononuclear Phagocytes

      • Philippe Poindron, Yves Lombard
      Pages 240-263

    6. The Human Leukemia Cell Line HL-60 as a Cell Culture Model To Study Neutrophil Functions and Inflammatory Cell Responses

      • Faustino Mollinedo, Antonio M. Santos-Beneit, Consuelo Gajate
      Pages 264-297
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Keywords

About this book

Cell culture techniques allow a variety of molecular and cell biological questions to be addressed, offering physiological conditions whilst avoiding the use of laboratory animals. In addition to basic techniques, a wide range of specialised practical protocols covering the following areas are included: cell proliferation and death, in-vitro models for cell differentiation, in-vitro models for toxicology and pharmacology, industrial application of animal cell culture, genetic manipulation and analysis of human and animal cells in culture.

Editors and Affiliations

  • National Cell & Tissue Culture Center Bioresearch Ireland, Dublin City University, Glasnevin Dublin 9, Ireland

    Martin Clynes

Bibliographic Information

  • Book Title: Animal Cell Culture Techniques

  • Editors: Martin Clynes

  • Series Title: Springer Lab Manuals

  • DOI: https://doi.org/10.1007/978-3-642-80412-0

  • Publisher: Springer Berlin, Heidelberg

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer-Verlag Berlin Heidelberg 1998

  • Softcover ISBN: 978-3-540-63008-1Published: 16 June 1998

  • eBook ISBN: 978-3-642-80412-0Published: 06 December 2012

  • Edition Number: 1

  • Number of Pages: XIII, 618

  • Number of Illustrations: 34 b/w illustrations

  • Topics: Cell Biology, Human Physiology, Biochemistry, general

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