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Cytoskeleton Proteins

A Purification Manual

  • Book
  • © 1995

Overview

Authors:
  1. Gerhard Isenberg

    1. Lehrstuhl für Biophysik E22, Technische Universität München, Garching bei München, Germany

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Part of the book series: Springer Lab Manuals (SLM)

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Table of contents (12 chapters)

  1. Front Matter

    Pages i-1
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  2. General

    1. User’s Instructions

      • G. Isenberg
      Pages 2-3

    2. General Lab Equipment

      • Gerhard Isenberg
      Pages 4-5

    3. Preparation of Buffers

      • Gerhard Isenberg
      Pages 6-14

    4. Ammonium Sulfate Precipitation

      • Gerhard Isenberg
      Pages 15-16

    5. Chromatographic Fractionation

      • Gerhard Isenberg
      Pages 17-19

    6. Centrifugation

      • Gerhard Isenberg
      Pages 20-21

    7. Abbreviations

      • Gerhard Isenberg
      Pages 22-24

  3. Purification Protocols

    1. Actin and Actin-Associated Proteins

      • Gerhard Isenberg
      Pages 25-149

    2. Tubulin and Microtubule-Associated Proteins

      • Gerhard Isenberg
      Pages 151-181

    3. Motor Proteins

      • Gerhard Isenberg
      Pages 183-203

    4. Intermediate Filament Proteins

      • Gerhard Isenberg
      Pages 205-229

    5. Plasmamembrane and Organelle-Associated Cytoskeletal Proteins

      • Gerhard Isenberg
      Pages 231-257

  4. Back Matter

    Pages 258-269
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Keywords

About this book

The standard protocols for the purification of all known cytoskeleton proteins are presented in this manual. Proteins are listed alphabetically and each protocol follows a common format. Thus, the manual provides a quick and easy reference to all relevant procedures for cytoskeleton protein purification.
The isolation procedure for each protein is shown in a clear flowchart, while the source of the protein, equipment and material needed, a list of suppliers, standard references, accession No. of sequences as well as further relevant facts and practical tips are given on a separate page.

Reviews

"In one word, a perfect book, which we recommend highly, since necessary, without any exception, to all cellular and molecular laboratories, and also to the great majority of all other laboratories." Cellular and Molecular Biology

Authors and Affiliations

  • Lehrstuhl für Biophysik E22, Technische Universität München, Garching bei München, Germany

    Gerhard Isenberg

Bibliographic Information

  • Book Title: Cytoskeleton Proteins

  • Book Subtitle: A Purification Manual

  • Authors: Gerhard Isenberg

  • Series Title: Springer Lab Manuals

  • DOI: https://doi.org/10.1007/978-3-642-79632-6

  • Publisher: Springer Berlin, Heidelberg

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer-Verlag Berlin Heidelberg 1995

  • Softcover ISBN: 978-3-642-48961-7Published: 19 July 2012

  • eBook ISBN: 978-3-642-79632-6Published: 06 December 2012

  • Edition Number: 1

  • Number of Pages: IV, 269

  • Topics: Cell Biology, Biochemistry, general

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