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Gene Transfer to Plants

  • Book
  • © 1995

Overview

Editors:
  1. Ingo Potrykus

    1. Swiss Federal Institute of Technology, Institute of Plant Sciences, ETH Zürich, Zürich, Switzerland

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  2. German Spangenberg

    1. Swiss Federal Institute of Technology, Institute of Plant Sciences, ETH Zürich, Zürich, Switzerland

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Springer Lab Manuals (SLM)

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Table of contents (33 chapters)

  1. Front Matter

    Pages I-XXII
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  2. Agrobacterium tumefaciens-Mediated Gene Transfer

    1. Front Matter

      Pages 1-4
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  3. Agrobacterium rhizogenes-Mediated Gene Transfer

    1. Front Matter

      Pages 39-44
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  4. Direct Gene Transfer to Protoplasts

    1. Front Matter

      Pages 53-57
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    2. Protocol

      1. Polyethylene Glycol-Mediated Direct Gene Transfer to Tobacco Protoplasts and Regeneration of Transgenic Plants
        • G. Spangenberg, I. Potrykus
        Pages 58-65
      2. Polyethylene-Glycol-Mediated Direct Gene Transfer to Indica Rice Protoplasts and Regeneration of Transgenic Plants
        • S. K. Datta
        Pages 66-74
      3. Plastid Transformation by Polyethylene Glycol Treatment of Protoplasts and Regeneration of Transplastomic Tobacco Plants
        • H.-U. Koop, W. Kofer
        Pages 75-82
      4. Electroporation-Mediated Gene Transfer to Protoplasts and Regeneration of Transgenic Plants
        • M. G. K. Jones
        Pages 83-92
      5. Electroporation-Mediated Gene Transfer to Japonica Rice Protoplasts and Regeneration of Transgenic Plants
        • C. Wu, K. Shimamoto
        Pages 93-98
      6. Regeneration of Transgenic Maize Plants from Embryogenic Protoplasts After Polyethylene Glycol-Mediated DNA Uptake
        • S. Omirulleh, S. Mórocz, D. Dudits
        Pages 99-105
      7. Gene Transfer to Protoplasts: Transient Gene Expression Analysis
        • J. C. Carle Urioste, K. Marrs, J. Bodeau, V. Walbot
        Pages 106-111

  5. Biolistic Plant Transformation

    1. Front Matter

      Pages 113-117
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    2. Protocol

      1. Transient Gene Expression Studies with the Biolistic System
        • D. A. Russell, M. E. Fromm
        Pages 118-126
      2. Maize Transformation by Microprojectile Bombardment of Immature Embryos
        • E. Dunder, J. Dawson, J. Suttie, G. Pace
        Pages 127-138
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Keywords

About this book

Using the clearly structured protocols given in this manual, it will be easy to apply the latest techniques in plant biotechnology in order to create new plant varieties or varieties with altered and optimized characteristics.
Direct gene transfer into plant cells or protoplasts by the use of microinjection, electroporation or biolistic systems, or mediated by Agrobacteria is described in detail for various plant species, including relevant crops and cereals. Also included are protocols of the standard molecular techniques for the analysis of transgenic plants as well as a section on biosafety considerations and regulations.

Reviews

"The protocols are described as step-by-step procedures in such a way that everybody with some basic knowledge in biological experimentation will be able to repeat the experiments without much further reading."
Journal of Plant Physiology
"...well worth the investment."
Plant Growth Regulation

Editors and Affiliations

  • Swiss Federal Institute of Technology, Institute of Plant Sciences, ETH Zürich, Zürich, Switzerland

    Ingo Potrykus, German Spangenberg

Bibliographic Information

  • Book Title: Gene Transfer to Plants

  • Editors: Ingo Potrykus, German Spangenberg

  • Series Title: Springer Lab Manuals

  • DOI: https://doi.org/10.1007/978-3-642-79247-2

  • Publisher: Springer Berlin, Heidelberg

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer-Verlag Berlin Heidelberg 1995

  • Softcover ISBN: 978-3-642-48967-9Published: 03 October 2013

  • eBook ISBN: 978-3-642-79247-2Published: 29 June 2013

  • Edition Number: 1

  • Number of Pages: XXII, 362

  • Number of Illustrations: 66 b/w illustrations, 16 illustrations in colour

  • Topics: Cell Biology, Biotechnology, Plant Sciences, Food Science, Agriculture, Forestry

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