PCR Topics

1. Front Matter

   Pages I-XI

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2. Basic Methodology and Research Applications

   1. Front Matter

      Pages 1-1

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   2. Quantitation of mRNA by the Polymerase Chain Reaction

      • Alice M. Wang, Michael V. Doyle, David F. Mark

      Pages 3-8

   3. Sequencing of PCR Products — Analysis of Factor IX Genes and of Recombination Events in Immunoglobulin Genes

      • Jean-Marie Buerstedde, Steve S. Sommer

      Pages 9-14

   4. Alternative Methods for DNA Probing in Diagnosis: Ligase Chain Reaction (LCR)

      • Hartmut Hampl, Ronald A. Marshall, Timothy Perko, Natalie Solomon

      Pages 15-22

   5. Application of PCR to the Detection and Analysis of Point Mutations in the Human Factor VIII Gene

      • Lutz-Peter Berg, David S. Millar, Catherine B. Grundy, Kerstin Wieland, Jonathan K. Pattinson, Edward G. D. Tuddenham et al.

      Pages 23-31

   6. The Use of PCR in the Diagnosis of Muscle Disease

      • Jörg Ehrenpreis, Barbara Junkes, Martin Pfordt, Hans-Peter Vosberg

      Pages 32-40

   7. Taq DNA Polymerase-Synthesized Single-Stranded DNA Hybridization Probes and their Application in Northern Blotting and in situ Hybridization

      • Michael Stürzl, Willy Kurt Roth, Petra Viehweger, Peter-Hans Hofschneider

      Pages 41-45

   8. Computer Program for the Selection of PCR Primers

      • G. Röllich, M. Jafari

      Pages 46-47

   9. Nervous System Cross Reactivity Sequences

      • M. E. Westarp, B. Föhring

      Pages 48-52

   10. The Use of Short Adapters for Priming PCR of Unknown Chromosomal DNA Fragments

       • J. Schneider, H. Schrempf

       Pages 53-55

   11. Labeling of Specific DNA Sequences with Digoxigenin during Polymerase Chain Reaction

       • Rüdiger Rüger, Hans-Joachim Höltke, Udo Reischl, Gregor Sagner, Christoph Kessler

       Pages 56-58

   12. Quantitative Analysis of Polymerase Chain Reaction (PCR) Products by Means of Fluorescence Detection

       • Axel Landgraf, Alfred Pingoud, Bernd Reckmann

       Pages 59-62

   13. Reverse Transcription PCR (RT-PCR) of Parathyroid Hormone (PTH) mRNA

       • Oliva Handt, André Reis, Rudolf Häring, Jörg Schmidtke

       Pages 63-65

   14. False Positive Reactions in PCR

       • Erik C. Böttger

       Pages 66-68

   15. Error Production and Error Propagation During PCR

       • Armin Wagner, Bernd Reckmann, Kerstin Hagen-Mann, Gerhard Krauss

       Pages 69-71

3. Genetics and Evolution

   1. Front Matter

      Pages 73-73

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   2. PCR in Linkage Analysis of Genetic Diseases

      • Andre Reis

      Pages 75-79

   3. Amplification, Isolation and Direct Nucleotide Determination of Entire Genes: Application to the Study of 16S rRNAs for Molecular Evolution in Bacteria, Identification of Cultural Isolates and Development of Probes

      • B. Böddinghaus, T. Rogall, T. Flohr, H. Blöcker, J. Wolters, E. C. Böttger

      Pages 80-83

   4. Diagnosis of Cystic Fibrosis — Detection of the Gene Deletion Delta F 508

      • H. Ehrfeld, C. Bottner, R. Seelig, M. Renz

      Pages 84-88

PCR, developed at Cetus Corporation/USA by Henry A. Erlich, Kary Mullis and Randall K. Saiki, is a very simple method for amplifying nucleic acids in vitro. The realization of this idea bases on the repetition of a set of three different temperatures and yields an increase of the target structure up to a factor of 106 to 1012. Therefore, this technique is predisposed for safe analysis and characterization of DNA and RNA sequences of interest, even where the starting amount of material is enormously small. Because of its sensitivity, speed and versatility this method is particularly suitable for investigations of oncogenes, tumor associated translocations, retroviral sequences, lymphokines and mainly the broad field of degenerative and inflammatory diseases of nervous system. PCR seems to be the technique which could overcome the two most important problems in that field: very small amount of material combined with the necessity of rapid diagnostic procedures in inflammatory infections. "PCR topics" will give an actual overview of basic and applied research fields on usage of polymerase chain reaction. All contributions to this book have been presented at an international congress on "Usage of Polymerase chain reaction in genetic and infectious diseases" which took place in june 1990 in Berlin. The editors wish to thank all participants for their contributions. We offer our thanks and gratitude to our coworkers and especially to our technical assistents Barbara Trampenau, Mirjana Wiirdemann and Hannelore Leonhard.

• DNA detection methods
• Polymerase chain reaction (PCR)
• Viral diseases
• AIDS
• antigen
• bacteria
• diseases
• genetics
• Hepatitis
• infection
• infections
• infectious
• infectious disease
• infectious diseases
• lymphokine
• retroviruses
• tuberculosis
• virus

• Neurologische Klinik des Klinikum Steglitz, FU Berlin, Berlin 45, Germany

  Arndt Rolfs, Hermann Christian Schumacher, Peter Marx

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