Transgenic Mice and Mutants in MHC Research

1. Front Matter

   Pages I-XV

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2. Mutant Models of MHC Antigen Expression and Function (H-2)

   1. Front Matter

      Pages 1-1

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   2. Spontaneous Frequency of H-2 Mutations

      • R. W. Melvold, H. I. Kohn

      Pages 3-13

   3. Microgene Conversion in the Evolution of the MHC Class I Multigene Family

      • C. Wheeler, D. Maloney, S. Watts, J. Vogel, J. Towner, D. Baldwin et al.

      Pages 14-25

   4. Random Mutagenesis by Oligonucleotides: A Probe for MHC Structure and Function

      • K. Katoh, R. Murray, D. Muller, J. A. Frelinger

      Pages 26-32

   5. Altered Recognition of LCMV Antigen Presented on Mutant Class I Molecules

      • D. Muller, M. M. Hobbs, R. Murray, K. Pederson, R. Ahmed, J. A. Frelinger

      Pages 33-35

   6. Relationship Between Class II Structure and B Cell Signaling

      • G. A. Bishop, J. A. Frelinger

      Pages 36-38

   7. Multivalent Requirement for the Stimulation of Alloreactive T Cells: Studies with Engineered Soluble MHC Class I Proteins In Vitro and In Vivo

      • D. H. Margulies, L. F. Boyd, S. Kozlowski, L. Kjer-Nielsen, R. Lopez, J. McCluskey et al.

      Pages 39-46

   8. Novel MHC Variants Spliced by Overlap Extension

      • H. D. Hunt, J. K. Pullen, Z. Cai, R. M. Horton, S. N. Ho, L. R. Pease

      Pages 47-55

   9. The Functional Significance of Amino Acid Polymorphisms in Class I MHC Molecules

      • J. K. Pullen, H. D. Hunt, R. M. Horton, L. R. Pease

      Pages 56-58

   10. Products of Separate Genes Encoded Within Minor Histocompatibility Loci Stimulate Class I and Class II MHC-Restricted T Cells

       • D. C. Roopenian, A. P. Davis

       Pages 59-61

   11. Determination of Distinction Odortypes by Mutation of Class I and Class II MHC Genes

       • K. Yamazaki, G. K. Beauchamp, L. Thomas, J. Bard, E. A. Boyse

       Pages 62-64

3. Mutants of MHC Genes as Probes of Immunological Function (HLA)

   1. Front Matter

      Pages 65-65

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   2. Analysis of HLA-B27-Specific T-Cell Epitopes with Site-Directed Mutants Mimicking HLA-B27 Polymorphism

      • S. Rojo, V. Calvo, D. López, B. Galocha, J. A. López de Castro

      Pages 67-76

   3. The Role of Amino Acid Position and Side Chain Structure in Serological and CTL-Defined Epitopes on the HLA-A2.1 Molecule

      • K. T. Hogan, C. Clayberger, N. Shimojo, W. E. Biddison, A. M. Krensky, V. H. Engelhard

      Pages 77-88

   4. Two Amino Acids on the α Helical Region of the α1 Domain Form Multiple Epitopes Recognized by HLA-Bw52 Specific Human Cytotoxic T Cell

      • M. Takiguchi, J. Yamamoto, H. Hayashi, K. Kano

      Pages 89-90

4. Regulation of MHC Gene Expression

   1. Front Matter

      Pages 91-91

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   2. Mouse Mammary Tumor Virus-Directed Gene Expression in Transgenic Mice

      • S. R. Ross, Y. Choi, C.-L. L. Hsu, J. Dudley

      Pages 93-101

   3. Regulation of Class I MHC Expression: In Vivo Function of Regulatory DNA Sequence Elements in Transgenic Mice

      • J. E. Maguire, R. Ehrlich, W. I. Frels, D. S. Singer

      Pages 102-108

   4. Expression of a Non-Classical Class I Gene in Transgenic Mice

      • C. Bordallo, W. I. Frels, J. Lunney, A. Rosenberg, S. Rudikoff, D. S. Singer

      Pages 109-113

This volume contains papers presented at the international conference on "Transgenic Mice and Mutants in MHC Research", held in Bar Harbor, Maine, in June, 1989. The meeting brought together eighty researchers working in the field. While mouse H-2 mutants have been known for many years their studies continue to contribute a great deal to our understanding of structure/function relationships and evolution of MHC molecules. Recently a new direction of research has emerged on regulation, expression and function of MHC genes using transgenic animals carrying exogenous MHC genes from the same or other species or engineered MHC genes. With the introduction of transgenic mice more specific questions about the various functions of MHC genes can be answered as, for example, the role of soluble and membrane-bound MHC molecules in self tolerance and elimination of specific T cell clones, recognition of human MHC antigens by the mouse immune system, the role of individual human MHC genes in disease susceptibility. New approaches for evaluation of the role of MHC linked and unlinked genes in susceptibility of mice to malignant tumors and their metastases have been also reported. Our purpose has been to provide a forum for discussion of these new developments. Many questions remain to be answered but the necessary tools have become available. We thank the contributors and hope that the readers will benefit from this Pro­ ceedings. We wish to acknowledge the financial sponsorship for the meeting by The Pew Charitable Trust and Howard Hughes Medical Institute.

• MHC
• T cells
• disease association
• genes
• transgenic animal gene expression

• The Jackson Laboratory, Bar Harbor, USA

  Igor K. Egorov

• Department of Immunology, Mayo Medical School, Mayo Clinic, Rochester, USA

  Chella S. David

Policies and ethics