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Cell Culture Techniques in Heart and Vessel Research

  • Book
  • © 1990

Overview

Editors:
  1. H. M. Piper

    1. Physiologisches Institut, Abteilung für Herz- und Kreislaufphysiologie der Universität Düsseldorf, Düsseldorf 1, Germany

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Table of contents (23 chapters)

  1. Front Matter

    Pages I-XIII
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  2. Heart Muscle Cells

    1. Front Matter

      Pages 1-1
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    2. Embryonic Chick Heart Muscle Cells

      • A. C. Nag
      Pages 3-19

    3. Neonatal Rat Heart Muscle Cells

      • A. Pinson
      Pages 20-35

    4. Adult Ventricular Rat Heart Muscle Cells

      • H. M. Piper, A. Volz, P. Schwartz
      Pages 36-60

    5. Adult Atrial Cardiocytes in Culture

      • M. Cantin
      Pages 61-74

    6. Muscle Cell Cultures from Human Heart

      • S. L. Jacobson, R. A. Altschuld, C. M. Hohl
      Pages 75-98

    7. Attachment Substrates for Heart Muscle Cells

      • T. K. Borg, L. Terracio
      Pages 99-107

    8. Microcarrier Culture of Neonatal Heart Cells

      • P. A. Uusimaa, I. E. Hassinen
      Pages 108-114

  3. Endothelial Cells

    1. Front Matter

      Pages 115-115
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    2. Macrovascular Endothelial Cells from Porcine Aorta

      • A. M. Rosenthal, A. I. Gotlieb
      Pages 117-129

    3. Microvascular Endothelial Cells from the Lungs

      • U. S. Ryan
      Pages 130-139

    4. Microvascular Endothelial Cells from Brain

      • P. D. Bowman, M. du Bois, K. Dorovini-Zis, R. R. Shivers
      Pages 140-157

    5. Microvascular Endothelial Cells from Heart

      • H. M. Piper, R. Spahr, S. Mertens, A. Krützfeldt, H. Watanabe
      Pages 158-177

    6. Macro- and Microvascular Endothelial Cells from Human Tissues

      • V. W. M. van Hinsbergh, M. A. Scheffer, E. G. Langeler
      Pages 178-204

    7. Vascular Endothelial Cell-Synthesized Extracellular Matrices as Attachment Substrates In Vitro

      • I. M. Herman
      Pages 205-211

    8. Endothelial Cells Grown on Filter Membranes

      • D. M. Shasby, S. S. Shasby
      Pages 212-219

    9. Microcarrier Cultures of Endothelial Cells

      • R. Spahr, H. M. Piper
      Pages 220-229

    10. Growth Factors for Vascular Endothelial Cells

      • D. Gospodarowicz
      Pages 230-244

  4. Smooth Muscle Cells and Pericytes

    1. Front Matter

      Pages 245-245
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Keywords

About this book

In the cardiovascular sciences, an increasing demand for the use of modern methods of cell biology has developed. The use of specific cell culture models of the various tissues involved is essential for most of these novel approaches. This book meets the demand for acomprehensive and easy accessible source for cell cul- ture methods in cardiovascular research as it was not previously available. The basic methods for cultures of cardiomyocytes (embryonic and adult), endothelial cells (micro- and macrovascular), smooth muscle cells and pericytes are described in detail by an international selection of experts. Special chapters discuss the use of growth factors and attachment substrates, techniques for co-cultures, cultures on permeable filter membranes and microcarrier cultures. The methodological descriptions are sufficiently detailed for an immediate application in the laboratory. All chapters also contain a critical evaluation of alternative approaches.

Editors and Affiliations

  • Physiologisches Institut, Abteilung für Herz- und Kreislaufphysiologie der Universität Düsseldorf, Düsseldorf 1, Germany

    H. M. Piper

Bibliographic Information

  • Book Title: Cell Culture Techniques in Heart and Vessel Research

  • Editors: H. M. Piper

  • DOI: https://doi.org/10.1007/978-3-642-75262-9

  • Publisher: Springer Berlin, Heidelberg

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer-Verlag Berlin Heidelberg 1990

  • Softcover ISBN: 978-3-642-75264-3Published: 23 December 2011

  • eBook ISBN: 978-3-642-75262-9Published: 06 December 2012

  • Edition Number: 1

  • Number of Pages: XIV, 362

  • Topics: Cardiology, Human Physiology, Biochemistry, general, Pharmacology/Toxicology

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