The Early Effects of Radiation on DNA

1. Front Matter

   Pages I-XII

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2. The Interdisciplinary Approach to Radiation Action

   1. Mechanisms of Radiation Action on DNA in Model Systems - Their Relevance to Cellular DNA

      • J. F. Ward

      Pages 1-16

   2. Biophysical Models of Radiation Action - Development of Simulation Codes

      • Herwig G. Paretzke

      Pages 17-32

   3. Links Between Radiation Track Structure, Radiochemical Species, and Cell Survival

      • D. J. Brenner, C. R. Geard

      Pages 33-48

   4. A Kinetic Investigation of the Mechanisms of Radiation-Induced Strand Breakage in DNA Model Systems

      • George D. D. Jones, Peter O’Neill

      Pages 49-50

   5. Discussion on Interdisciplinary Approach to Radiation Action

      • P. O’Neill

      Pages 51-54

3. Cellular DNA Strand Breakage

   1. Measurement of DNA Double Strand Breaks in Mammalian Cells: Comparison Between Pulsed Field Gel Electrophoresis and Non-Unwinding Filter Elution

      • George Iliakis, Lorenz Metzger, Ryuichi Okayasu, Gabriel Pantelias, Orlando Cicilioni

      Pages 55-69

   2. A Mouse Lymphoma Line that Undergoes Rapid Interphase Death (Apoptosis) Shows Extreme Sensitivity to Radiation-Induced DNA Double-Strand Breakage

      • Ian R. Radford

      Pages 71-84

   3. The Influence of Genome Structural Organization on DNA Damage and Repair in Eukaryotic Cells Exposed to Ionizing Radiation

      • O. Sapora, M. Belli, B. Maione, S. Pazzaglia, M. A. Tabocchini

      Pages 85-101

   4. The Lethality of Radiation-Induced DNA Double-Strand Breaks for Radiations of Differing LET

      • Kevin M. Prise, Melvyn Folkard, Susan Davies, Barry D. Michael

      Pages 103-104

   5. Single-Strand Breaks and Base Damage in DNA of Human White Blood Cells in Full Blood Exposed to Ionizing Radiation Detected at Biologically Relevant Doses

      • A. A. W. M. van Loon, G. P. van der Schans, A. J. Timmerman, F. J. A. Kouwenberg, R. H. Groenendijk, P. H. M. Lohman et al.

      Pages 105-106

   6. Discussion: Cellular DNA Strand Breakage

      • P. L. Olive

      Pages 107-110

4. Early Chemical Events in DNA Damage

   1. The Role of Radiation Induced Charge Migration with DNA: ESR Studies

      • Martyn C. R. Symons

      Pages 111-124

   2. Early Chemical Events in the Development of Radiation Damage of DNA - Novel Approaches

      • P. O’Neill, A. T. Al-Kazwini, E. J. Land, E. M. Fielden

      Pages 125-140

   3. Initial Sites of One Electron Attachment in DNA

      • William A. Bernhard

      Pages 141-154

   4. Molecular Orbital Calculations on DNA Base Pairs

      • D. M. Close

      Pages 155-156

   5. Role of Energy and Charge Transfer in DNA Damage by Densely Ionizing Radiation

      • John H. Miller

      Pages 157-158

   6. Early Cemical Events in DNA Damage

      • Jürgen Hüttermann

      Pages 159-162

5. Development of Radiation Models

   1. Concepts of Microdosimetry and their Applicability to DNA Studies

      • Joachim Breckow, Albrecht M. Kellerer

      Pages 163-178

   2. The Application of Biophysical Models to Cellular DNA Damage

      • D. E. Charlton

      Pages 179-193

Interest in the biological effects of ionising radiation closely followed the identification of such radiation. The realisation that DNA is the site of genetic infonnation in cells subsequently focussed attention on DNA as an important target in the lethal and mutagenic effects of ionising radiation. Thus radiation effects upon DNA became an important area for fundamental scientific studies by radiation biologists, chemists and physicists. To a first approximation, the concerns of the three disciplines can be divided by time scales: the physical process of energy deposition from photon or charged 16 12 particle and subsequent relaxation (-10- to 10- secs), followed by chemical 12 2 reactions (- 10- to 10 secs), and fmally, the expression of biological effect (minutes to years). Thus, the concept of 'early processes' conveys different ideas to different scientists, although they are all interrelated. To attempt to describe in any detail all these processes is a mammoth task which is not made easier by the different conventions and experimental approaches of the three disciplines. However, the recent advances in all these scientific areas seemed, to the organisers at least, to offer the opportunity to stimulate more active interaction between physicists, chemists and biologists. With this in mind, a multi-disciplinary workshop was organised, which brought together some fifty scientists to present their own specialist interests and, through extensive discussion, explore which problems are of high priority and require input from the different disciplines to resolve them.

• Chromosom
• DNA
• Erbgut
• Mutation
• Radiation
• Strahlung

• Medical Research Council, Radiobiology Unit, Chilton, Didcot, Oxon, UK

  E. M. Fielden, P. O’Neill

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