Monoclonal Antibodies

1. Front Matter

   Pages I-XVII

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2. Introduction

   • J. H. Peters, D. Baron

   Pages 1-17

3. Preconditions for Hybridoma Technology

   • A. Wiggenhauser, J. H. Peters, H. Baumgarten

   Pages 18-38

4. Immunization

   • H. Baumgarten, M. Schulze, J. H. Peters, T. Hebell

   Pages 39-70

5. Taking Blood and Isolating Cells

   • A. Wiggenhauser, J. H. Peters, H. Baumgarten, A. Borgya

   Pages 71-87

6. Cell Culture

   • J. H. Peters, E. Debus, H. Baumgarten, R. Würzner, M. Schulze, Helga Gerlach

   Pages 88-136

7. Production of Hybridomas

   • D. Baron, J. H. Peters, R. K. H. Gieseler, S. Lenzner, H. Baumgarten, R. Würzner et al.

   Pages 137-222

8. Mass Production of Monoclonal Antibodies

   • H. Baumgarten, R. Franze, J. H. Peters, A. Borgya, D. Baron, E. Debus et al.

   Pages 223-257

9. Purifying Monoclonal Antibodies and Producing Antibody Fragments

   • M. Oppermann, R. Würzner, M. Schulze, H. Baumgarten, R. Zierz

   Pages 258-284

10. Coupling Monoclonal Antibodies

    • W. Wörner, F. Bieber, R. Zierz, Th. Hebell

    Pages 285-315

11. Demonstration of Monoclonal Antibodies

    • J. H. Peters, M. Schulze, M. Grol, S. Schiefer, H. Baumgarten, J. Endl et al.

    Pages 316-461

12. Safety Precautions at Work

    • D. Baron

    Pages 463-465

13. Appendix

    • Johann Hinrich Peters, Horst Baumgarten

    Pages 466-474

14. Back Matter

    Pages 475-488

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The present new version of this popular laboratory manual is at the same time the first one of this text in the English language - and this makes me even a little proud, as it reminds me of probably the first collection of monoclonal recipes in English, written by myself, which circulated for a couple of years in many laboratories. In the meantime many researchers have put enormous effort into improving methods for monoclonal antibody production. The proce­ dures have become more and more standardized and by this have more and more lost the character of magic secrets. Hinrich Peters and Horst Baumgarten, who had followed this good tradition already in the previous edition, written in German, suc­ ceeded in making laboratory tricks teachable. They had contributed their own experiences in cell culture and immunology, and were able to engage a number of experienced authors to contribute to the work. They were all willing to follow the general concept of this book, which contains a brief theoretical background for the methods described and presents the procedures in a highly organized structure. So the book has retained its shape as a "cook-book", which I especially like.

• Antibodies
• Antikörper
• Cell Culture
• Hybridoma
• Immunisieren
• Immunization
• Immunoassay
• Immunotest
• Monoclonal Antibodies
• Zellkultur

• Abteilung für Immunologie, Universität Göttingen, Göttingen, Germany

  Johann Hinrich Peters

• Forschungszentrum Tutzing, Boehringer Mannheim GmbH, Tutzing, Germany

  Horst Baumgarten

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