Radioimmunoassay in Basic and Clinical Pharmacology

1. Front Matter

   Pages I-XXII

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2. Radioimmunoassay: Historical Aspects and General Considerations

   • R. S. Yalow

   Pages 1-6

3. Basic Principles of Antigen-Antibody Interaction

   • F. Celada

   Pages 7-22

4. Production of Antisera by Conventional Techniques

   • G. Ciabattoni

   Pages 23-68

5. Production of Monoclonal Antibodies for Radioimmunoassays

   • K. Brune, M. Reinke

   Pages 69-89

6. Radioiodination and Other Labeling Techniques

   • J. Grassi, J. Maclouf, P. Pradelles

   Pages 91-141

7. Strategies for Developing Specific and Sensitive Hapten Radioimmunoassays

   • E. Ezan, S. Mamas, C. Rougeot, F. Dray

   Pages 143-179

8. How to Improve the Sensitivity of a Radioimmunoassay

   • G. Ciabattoni

   Pages 181-191

9. Statistical Aspects of Radioimmunoassay

   • D. Rodbard, V. Guardabasso, P. J. Munson

   Pages 193-212

10. Validation Criteria for Radioimmunoassay

    • C. Patrono

    Pages 213-225

11. Measurement of Opioid Peptides in Biologic Fluids by Radioimmunoassay

    • F. Nyberg, I. Christensson-Nylander, L. Terenius

    Pages 227-253

12. Radioimmunoassay of Pituitary and Hypothalamic Hormones

    • D. Cocchi

    Pages 255-290

13. Radioimmunoassay of Nonpituitary Peptide Hormones

    • G. Nilsson

    Pages 291-314

14. Radioimmunoassay of Gastrointestinal Polypeptides

    • J. H. Walsh, H. C. Wong

    Pages 315-350

15. Radioimmunoassay of Atrial Peptide Blood and Tissue Levels

    • M. L. Michener, D. Schwartz, M. G. Currie, D. M. Geller, P. Needleman

    Pages 351-362

16. Immunochemical Methods for Adrenal and Gonadal Steroids

    • M. Pazzagli, M. Serio

    Pages 363-400

17. Radioimmunoassay of Thyroid Hormones

    • L. Bartalena, S. Mariotti, A. Pinchera

    Pages 401-431

18. Radioimmunoassay of Catecholamines

    • E. Knoll, H. Wisser

    Pages 433-448

19. Radioimmunoassay of Prostaglandins and Other Cyclooxygenase Products of Arachidonate Metabolism

    • B. A. Peskar, Th. Simmet, B. M. Peskar

    Pages 449-480

20. Radioimmunoassay of Leukotrienes and Other Lipoxygenase Products of Arachidonate

    • H. J. Zweerink, G. A. Limjuco, E. C. Hayes

    Pages 481-500

Thirty years have elapsed since the first description by S. A. BERSON and R. S. Y ALOW of the basic principles of radioimmunoassay (RIA). During this period of time, RIA methodology has been instrumental to the growth of many areas of biomedical research, including endocrinology, oncology, hematology, and pharmacology. It has done so by providing a relatively simple universal tool allowing, for the first time, the detection of endogenous mediators that are present 12 10 in body fluids at concentrations as low as 10- _10- M. The fundamental nature of this discovery and the wide-ranging fall-out of basic and clinical knowledge derived from its application have been acknowledged by the many honors tributed to its pioneers, including the Nobel Prize awarded to Dr. Y ALOW 10 years ago. Although several excellent books have been published during the past decades covering various aspects of RIA methodology, we felt the need, as pharmacologists, for a comprehensive discussion of the methodological and conceptual issues related to the main classes of mediators of drug action and to drugs themselves. Thus, we gladly accepted the challenge provided by the invitation to edit a volume of the Handbook of Experimental Pharmacology on Radioimmunoassay in Basic and Clinical Pharmacology. We tried to balance the emphasis placed on more general aspects of the RIA methodology and that on specific mediators.

• drug
• endocrinology
• pharmacology
• research
• metabolic disease

• Institute of Pharmacology, Catholic University of the Sacred Heart, Rome, Italy

  Carlo Patrono

• Institute of Pharmacology and Toxicology, Ruhr-University Bochum Im Lottental, Bochum 1, Germany

  Bernhard A. Peskar

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