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Membrane Proteins

Isolation and Characterization

  • Book
  • © 1986

Overview

Editors:
  1. Angelo Azzi

    1. Institut für Biochemie und Molekularbiologie, Universität Bern, Bern, Switzerland

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  2. Lanfranco Masotti

    1. Istituto di Chimica Biologica, Università di Parma, Parma, Italy

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  3. Arnaldo Vecli

    1. Istituto di Fisica, Università di Parma, Parma, Italy

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Table of contents (19 chapters)

  1. Front Matter

    Pages I-XII
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  2. Membrane Proteins: Function, Purification, and Characterization

    1. Front Matter

      Pages 1-1
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    2. Purification of Cytochrome c Reductase and Oxidase by Affinity Chromatography

      • Reinhard Bolli, Clemens Broger, Angelo Azzi
      Pages 3-10

    3. Molecular Weight Estimation of Membrane Proteins: Gel Permeation Chromatography and Sucrose Gradient Centrifugation

      • Katarzyna A. Nałȩcz, Reinhard Bolli, Angelo Azzi
      Pages 11-23

    4. The Problem of Light Scattering in the Absorbance and Fluorescence Studies of Proteins in Membranes

      • P. Cavatorta, E. Casali, G. Sartor
      Pages 24-31

    5. Reproducible Preparation of Phosphatidylserine Vesicles for Fluorescence Studies of Protein Incorporation into Lipids

      • Janet Johns, Arthur G. Szabo
      Pages 32-41

    6. Purification and Reconstitution of the Proton Translocating Membrane Bound Inorganic Pyrophosphatase from Rhodospirillum rubrum

      • M. Baltscheffsky, P. Nyrén
      Pages 42-48

    7. The Function of Transmembrane Channels: Ion Transport Studies by 23Na NMR

      • A. Spisni, V. Comaschi, L. Franzoni
      Pages 49-55

    8. Membrane Signal Transduction via Protein Kinase C

      • C. W. Mahoney, R. Lüthy, A. Azzi
      Pages 56-66

    9. Extraction, Partial Purification, and Reconstitution of a Mixture of Carriers from the Inner Mitochondrial Membrane

      • Maciej J. Nałȩcz, Katarzyna A. Nałȩcz, Angelo Azzi
      Pages 67-75

  3. Lipid-Protein Interaction

    1. Front Matter

      Pages 77-77
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    2. The Effect of Polypeptides on the Fluidity of Lipid Bilayers

      • G. Sartor, M. B. Ferrari, P. Cavatorta
      Pages 79-85

    3. Polypeptide-Lipid Interactions as Studied by 13C NMR

      • A. Spisni, G. Farruggia, L. Franzoni
      Pages 86-94

    4. Conformational Changes in Polypeptides and Proteins Brought About by Interactions with Lipids

      • L. Masotti, J. Von Berger, N. Gesmundo
      Pages 95-106

  5. Protein Reconstitution

    1. Front Matter

      Pages 149-149
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Keywords

About this book

This volume is the third of a series on Membrane Proteins and, like the pre­ ceding manuals, is the result of an International Advanced Course entitled Isolation and Characterization of Membrane Proteins: Biochemical and Bio­ physical Aspects sponsored by the Federation of European Biochemical So­ cieties (FEBS) and the Italian Research Council (CNR). The success of the course and the continuous development in the field of membrane biology has prompted me to publish also in this case the protocols of the experiments which were carried out by the students. The students have been able not only to perform the experiments pub­ lished in this manual without help from the instructors, but also to suggest improvements, which have been incorporated in the published version. Care has been taken in making the planning and the execution of the ex­ periments as simple as possible, by listing in detail all the necessary pieces of equipment, test tubes, pipettes, chemicals, etc. At the same time the intro­ duction and the "philosophy" have been limited to the essential, as also the references, only those having been listed which may help in a better under­ standing of the principles and of the biological background of a given experi­ ment.

Editors and Affiliations

  • Institut für Biochemie und Molekularbiologie, Universität Bern, Bern, Switzerland

    Angelo Azzi

  • Istituto di Chimica Biologica, Università di Parma, Parma, Italy

    Lanfranco Masotti

  • Istituto di Fisica, Università di Parma, Parma, Italy

    Arnaldo Vecli

Bibliographic Information

  • Book Title: Membrane Proteins

  • Book Subtitle: Isolation and Characterization

  • Editors: Angelo Azzi, Lanfranco Masotti, Arnaldo Vecli

  • DOI: https://doi.org/10.1007/978-3-642-71543-3

  • Publisher: Springer Berlin, Heidelberg

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer-Verlag Berlin Heidelberg 1986

  • Softcover ISBN: 978-3-540-17014-3Published: 01 December 1986

  • eBook ISBN: 978-3-642-71543-3Published: 06 December 2012

  • Edition Number: 1

  • Number of Pages: XII, 182

  • Number of Illustrations: 3 b/w illustrations

  • Topics: Biochemistry, general, Biological and Medical Physics, Biophysics

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