Overview
- Editors:
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Brigitte Wittmann-Liebold
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Abteilung Wittmann, Max-Planck-Institut für Molekulare Biologie, Berlin 33, Germany
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Johann Salnikow
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Institut für Biochemie und Molekulare Biologie, Technische Universität Berlin, Berlin 10, Germany
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Volker A. Erdmann
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Institut für Biochemie, Freie Universität Berlin, Berlin 33, Germany
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Table of contents (32 chapters)
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Introduction
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- Brigitte Wittmann-Liebold, Johann Salnikow, Volker A. Erdmann
Pages 1-5
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Separation and Amino Acid Analysis of Proteins and Peptides for Microsequencing Studies
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- Heinz-Jürgen Brockmöller, Roza Maria Kamp
Pages 34-44
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- Tomas Bergman, Mats Carlquist, Hans Jörnvall
Pages 45-55
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- Rene Knecht, Chang Jui-Yoa
Pages 56-61
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Manual and Solid-Phase Microsequencing Methods
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- Carl C. Kuhn, John W. Crabb
Pages 64-76
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- Brigitte Wittmann-Liebold, Hisashi Hirano, Makoto Kimura
Pages 77-90
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- Werner Machleidt, Ursula Borchart, Anka Ritonja
Pages 91-107
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Gas-Phase and Radio-Sequence Analysis
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Front Matter
Pages 117-117
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- Frank Reimann, Brigitte Wittmann-Liebold
Pages 118-125
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- Lothar Meinecke, Harald Tschesche
Pages 126-148
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- Heinrich Gausepohl, Marcus Trosin, Rainer Frank
Pages 149-160
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- Wolf F. Brandt, Claus von Holt
Pages 161-178
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- Joel Vandekerckhove, Guy Bauw, Magda Puype, Jozef van Damme, Marc van Montagu
Pages 179-193
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About this book
Much of the recent spectacular progress in the biological sciences can be at tributed ot the ability to isolate, analyze, and structurally characterize proteins and peptides which are present in cells and cellular organelles in only very small amounts. Recent advances in protein chemistry and in particular the application of new micromethods have led to fruitful advances in the understanding of basic cellular processes. Areas where protein-chemical studies have resulted in interest ing discoveries include the peptide hormones and their release factors, growth factors and oncogenes, bioenergetics, proton pumps and ion pumps and chan nels, topogenesis and protein secretion, molecular virology and immunology, membrane protein analysis, and receptor research. In fact, the key methods are now on hand to unravel many of the major outstanding problems of molecular biology and in particular questions of fundamental interest which relate to devel opmental biology and specificity in cell-cell interaction. In this volume we have assembled descriptions of procedures which have re cently been shown to be efficaceous for the isolation, purification, and chemical characterization of proteins and peptides that are only available in minute amounts. Emphasis is placed on well-established micromethods which have been tested and found useful in many laboratories by experienced investigators. The chapters are written by specialists, and describe a range of sensitive techniques which can be used by researchers working in laboratories with only modest resources and equipment.
