Ultraviolet Spectroscopy of Proteins

1. Front Matter

   Pages I-XI

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2. Introduction

   • Alexander P. Demchenko

   Pages 1-4

3. Spectroscopic Properties of Protein Chromophores

   • Alexander P. Demchenko

   Pages 5-26

4. Display of Intramolecular and Intermolecular Interactions in Electronic Spectra of Amino Acids and Proteins

   • Alexander P. Demchenko

   Pages 27-63

5. Difference Spectra of Proteins

   • Alexander P. Demchenko

   Pages 64-90

6. Thermal Perturbation Difference Spectroscopy and Temperature-Dependent Conformational Transitions of Proteins

   • Alexander P. Demchenko

   Pages 91-120

7. Derivative Spectroscopy of Aromatic Amino Acids and Proteins

   • Alexander P. Demchenko

   Pages 121-136

8. Spectrophotometric Titration of Proteins

   • Alexander P. Demchenko

   Pages 137-144

9. Fluorescence Molecular Relaxation Spectroscopy

   • Alexander P. Demchenko

   Pages 145-172

10. Fluorescence Quenching

    • Alexander P. Demchenko

    Pages 173-182

11. Nonradiative Transfer of Electronic Excitation Energy

    • Alexander P. Demchenko

    Pages 183-197

12. Fluorescence Polarization and Rotational Mobility

    • Alexander P. Demchenko

    Pages 198-207

13. Intrinsic Phosphorescence of Proteins

    • Alexander P. Demchenko

    Pages 208-221

14. Employment of Ultraviolet Spectroscopy in Analytical Chemistry of Proteins

    • Alexander P. Demchenko

    Pages 222-240

15. Experimental Technique in Protein Spectroscopy

    • Alexander P. Demchenko

    Pages 241-262

16. General Conclusions and Prospects

    • Alexander P. Demchenko

    Pages 263-278

17. Back Matter

    Pages 279-312

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The aim of this book is to give a comprehensive description of the basic methods used in the ultraviolet spectroscopy of proteins, to discuss new trends and development of these methods, and to analyze their different applications in the study of various aspects of protein structure and dynamics. Ultraviolet spectroscopy is one of the oldest and most popular methods in the field of biochemistry and molecular biophysics. At present, it is difficult to imagine the biochemical laboratory without a recording spectrophotometer or spectrofluorimeter. There are several hundreds of publications directly devoted to protein ultraviolet spectroscopy and in a great number of studies UV spectroscopic methods are used for the structural analysis of different proteins. Meanwhile a unified description of the theoretical basis of the methods, experimental techniques, data analysis, and generalization of results obtained in solving the specific problems of protein structure are lacking. There are three reasons for which a monograph on ultraviolet spectroscopy is needed today. Firstly, there has been significant growth in facilities of experimental technique, its precision, and versatility associated with computer data analysts. This new technique is available to a wide circle of scientists engaged in the field of protein research. Most of them are not spectroscopists and, thus, there is a need for a conceivable and precise source of information on how to use this method and what kind of data it should provide.

• Photometer
• UV
• biochemistry
• biophysics
• protein
• proteins
• spectroscopy

• Palladin Institute of Biochemistry, Academy of Sciences of the Ukraine, Kiev, USSR

  Alexander P. Demchenko

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