The Impact of Gene Transfer Techniques in Eucaryotic Cell Biology

1. Front Matter

   Pages III-XI

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2. Introduction of DNA into Animal Cells and Its Use to Study Gene Function

   1. Front Matter

      Pages 1-1

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   2. Chemical DNA Synthesis and Its Applications in Eukaryotic Gene Transfer Techniques

      • E. L. Winnacker

      Pages 3-16

   3. An Alternative Gene Cloning Method for the Isolation of Human Genes by Expression in Mouse Cell Clones

      • W. Lindenmaier, H. Hauser, J. Collins

      Pages 17-25

   4. Enhancers as Transcriptional Control Elements

      • P. Gruss

      Pages 26-34

   5. Z-DNA: Conformational Flexibility of the DNA Double Helix

      • A. Nordheim

      Pages 35-46

3. DNA Rearrangements in Varions Organisms

   1. Front Matter

      Pages 47-47

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   2. Antigenic Variation in African Trypanosomes

      • H. Eisen, S. Longacre, G. Buck

      Pages 49-53

   3. The Plant Transposable Elements Tam1, Tam2 and Spm-I8

      • H. Saedler, U. Bonas, A. Gierl, B. J. Harrison, R. B. Klösgen, E. Krebbers et al.

      Pages 54-64

   4. Mobile Genetic Elements and Their Use for Gene Transfer in Drosophila

      • W. J. Gehring

      Pages 65-70

4. Genetic Engineering of Plants

   1. Front Matter

      Pages 71-71

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   2. Genetic Engineering of Plants

      • J. Schell, L. Herrera-Estrella, P. Zambryski, M. De Block, H. Joos, L. Willmitzer et al.

      Pages 73-90

   3. The Chloroplast Genome, Its Interaction with the Nucleus, and the Modification of Chloroplast Metabolism

      • P. H. Schreier, H. J. Bohnert

      Pages 91-102

   4. The T-Region of Ti Plasmid Codes for an Enzyme of Auxin Biosynthesis

      • J. Schröder, S. Waffenschmidt, E. W. Weiler, G. Schröder

      Pages 103-107

   5. Cauliflower Mosaic Virus: A Plant Gene Vector

      • B. Gronenborn

      Pages 108-118

5. Introduction of DNA into the Germ Line of Animals

   1. Front Matter

      Pages 119-119

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   2. Hybrid Dysgenesis as a Gene Transfer System in Drosophila melanogaster

      • D. J. Finnegan

      Pages 121-126

   3. Introducing Genes into Mice and into Embryonal Carcinoma Stem Cells

      • E. F. Wagner, U. Rüther, C. L. Stewart

      Pages 127-133

   4. Gene Transfer in Living Organisms

      • S. Rusconi

      Pages 134-152

6. Applications of Genetic Engineering

   1. Front Matter

      Pages 153-153

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The 35th N:osbach Colloquium "The Impact of Gene Transfer Techniques in Eukaryotic CeU Biology" brought together a number of speakers interested in various aspects of cellular and developmental biology and over 600 other scientists, who listened to the lectures and participated in the lively discussions. The questions and experiments described were very varied, but all of them illustrated the importance of recombinant DNA technology. The powerful techniques of identifying and isolating DNA sequences, followed by their introduction into living cells and even into the germ cells of multicellular organisms, have pervaded nearly every branch of molecular biology. The presentations and discussions that followed showed that recombinant DNA has tremendously increased our potential for fundamental research. Now, and for some time to corne, these contri­ butions and the resulting increase in our understanding of life will be the main result of gene manipulation. There will, however, also be applications that will lead to new industrial processes. One section was devoted to novel ways of vaccine production and another to herbicide resistance. These applications are a matter of intense debate in the public domain today. Although they reach beyond the scope of the research labora.tory at a university or research institution, scientists have the knowledge necessary to judge these developments and are sometimes directly involved. There­ fore the development of industrial qene technology requires the attention of the whole scientific community. We hope that this Symposium has also served this purpose.

• Biology
• Cell
• DNA
• cell biology
• developmental biology
• gene transfer

• Max-Planck-Institut für Züchtungsforschung, Köln 30, Germany

  Jozef Stephaan Schell

• Institut für Genetik, Universität Köln, Köln 41, Germany

  Peter Starlinger

Policies and ethics