Rapid Methods and Automation in Microbiology and Immunology

1. Front Matter

   Pages I-XVI

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2. Time Realities in the Evaluation of Vaccines for Safety and Efficacy

   1. The Evaluation of Vaccines

      • M. R. Hilleman

      Pages 1-5

   2. Evaluation of Hepatitis B Vaccine

      • S. Krugman

      Pages 6-12

   3. Concepts in the Development of Vaccines Against Genital Herpes Simplex Virus Infections

      • L. Corey, G. Mertz

      Pages 13-23

   4. Development of a Live Attenuated Vaccine Against Varicella

      • A. Gershon

      Pages 24-29

3. Hybridization Technology in Diagnostics

   1. The Use of DNA Hybridization for the Identification of Pathogenic Bacteria

      • S. Falkow

      Pages 30-33

   2. Microbial Diagnosis by Nucleic Acid Sandwich Hybridization

      • M. Ranki, M. Laaksonen, A. Palva, A.-C. Syvänen, M. Virtanen, H. Söderlund

      Pages 34-42

   3. Diagnosis of Papillomavirus Infection by Nucleic Acid Hybridization

      • L. Gissmann, A. Schneider

      Pages 43-47

   4. Detection of Epstein-Barr Virus DNA by Nucleic Acid Hybridization

      • G. W. Bornkamm, S. Wiest, C. Desgranges

      Pages 48-56

   5. Diagnosis of Viroid-Caused Plant Diseases by Nucleic Acid Hybridization

      • T. O. Diener, R. A. Owens

      Pages 57-67

   6. DNA Technology Applied to the Detection and Epidemiology of Enteric Pathogens

      • L. S. Tompkins, P. A. Mickelsen, N. Troup

      Pages 68-72

   7. New Developments in Nucleic Acid Hybridization

      • H. Wolf, M. Haus, U. Leser, S. Modrow, M. Motz, S.-Y. Gu et al.

      Pages 73-82

4. Application of Monoclonal Antibodies. Hybridoma Technology

   1. Variability of Antigen Epitopes of Monotypic Viruses

      • E. Norrby

      Pages 83-93

   2. Immunoblotting with Monoclonal Antibodies: A Highly Specific System for Detection and Identification of Bacterial Outer Membrane Proteins

      • M. Jürs, H. Peters, K. N. Timmis, D. Bitter-Suermann

      Pages 94-102

   3. Immunoblotting and Dot Immunobinding

      • J. Gordon, Ch. Rordorf, M. Rosenthal, Y. Z. Sun

      Pages 103-114

   4. Fluorescence-Activated Cell Sorter and Monoclonal Antibodies: Complementary Tools in Immunodiagnosis and Immunotherapy

      • T. J. Kipps, L. A. Herzenberg

      Pages 115-122

   5. A Microfilter Assay for the Detection of Antibody-Producing Cells In Vitro

      • S. A. Möller, C. A. K. Borrebaeck

      Pages 123-128

   6. A Biotin Avidin Amplified ELISA Using Exclusively Monoclonal Antibodies for the Detection of Respiratory Syncytial Viral Antigens in Nasopharyngeal Secretions

      • G. Obert, C. Beyer

      Pages 129-134

   7. Identification and Serotyping of Vibrio cholerae 0:1 by Monoclonal Antibody Based Enzyme-Linked Immunosorbent Assays

      • B. Gustafsson, T. Holme

      Pages 135-140

5. Rapid Diagnosis by IgM-Detection

   1. The Role of Specific Antibodies in Different Immunoglobulin Classes in Rapid Diagnosis of Virus Infections

      • U. Krech

      Pages 141-145

Rapid progress in molecular biology, genetic engineering, and basic research in immunology has opened up new possibilities for application to diagnostic procedures and to clinical research. In a short period a new era of diagnosis dawned, covering nearly all fields of microbiology, immunology, and food technology. In consequence of this rapid development, scientists of many disciplines are involved studying infections of humans, animals, and plants or working in technical microbiology. The application of the newest findings of basic research to diagnostic work and to clinical research covers nearly all fields of microbiology and immunology. Moreover, it underlines the close relationship between diagnosis, therapy, and epidemiology. An outstanding example of these connections is given by the recent development of hepatitis B vaccine. The discovery and identification of a non­ cultivable agent by physicochemical and immunological methods were the heralds of a new era in the prevention of infectious diseases. This book provides an up-to-date, comprehensive review of developments and future aspects in various fields. I am convinced that the authors have succeeded in furnishing a large variety of new ideas and possibilities. K.-O. HABERMEHL Contents Time Realities in the Evaluation of Vaccines for Safety and Efficacy The Evaluation of Vaccines M. R. HILLEMAN . . . . .

• Hepatitis
• diseases
• hepatitis B
• immunology
• infection
• infections
• infectious
• infectious disease
• infectious diseases
• microbiology
• molecular biology
• vaccine

• Institut für Klinische und Experimentelle Virologie, Freie Universität Berlin, Berlin 45, Germany

  K.-O. Habermehl

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