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Practice of High Performance Liquid Chromatography

Applications, Equipment and Quantitative Analysis

  • Book
  • © 1986

Overview

Editors:
  1. Heinz Engelhardt

    1. Angewandte Physikalische Chemie, Universität des Saarlandes, Saarbrücken, Germany

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Part of the book series: Chemical Laboratory Practice (CHEMICAL LABORA)

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Table of contents (15 chapters)

  1. Front Matter

    Pages I-XII
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  2. Liquid Chromatographic Equipment

    • Henri Colin, Georges Guiochon, Michel Martin
    Pages 1-64

  3. Quantitative Analysis in HPLC

    • Jürgen Asshauer, Helmut Ullner
    Pages 65-108

  4. Preparative Application of HPLC

    • A. Wehrli
    Pages 109-142

  5. Column-switching

    • Herwig Hulpke, Ulrich Werthmann
    Pages 143-157

  6. Sample Pretreatment and Cleanup

    • M. Uihlein
    Pages 159-178

  7. Liquid-liquid Chromatography

    • J. C. Kraak, J. P. Crombeen
    Pages 179-199

  8. Ion Pair Liquid Chromatography

    • B. A. Persson, B. L. Karger
    Pages 201-214

  9. Application of HPLC in Inorganic Chemistry

    • Hermann J. Möckel
    Pages 215-240

  10. HPLC in Forensic Chemistry

    • T. Daldrup, P. Michalke, S. Szathmary
    Pages 241-285

  11. Application of HPLC to the Separation of Lipids

    • K. Aitzetmüller
    Pages 287-321

  12. Application of HPLC to the Separation of Metabolites of Nucleic Acids in Physiological Fluids

    • Anne P. Halfpenny, Phyllis R. Brown
    Pages 323-342

  13. Application of HPLC to the Analysis of Natural and Synthetic Pharmaceutically Important Drugs

    • Gerolf Tittel, Hildebert Wagner
    Pages 343-391

  14. Application of HPLC for Analysis of Psychotropic Drugs in Body Fluids

    • Hans-Joachim Kuss
    Pages 393-407

  15. HPLC of Amino Acids and Proteins

    • H. Engelhardt
    Pages 409-425

  16. Chromatographic Methods in the Separation of Coal and Oil Products

    • Th. Crispin, I. Halász
    Pages 427-454

  17. Back Matter

    Pages 455-464
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Keywords

About this book

During its short 20 year history High Performance Liquid Chro­ matography (HPLC) has won itself a firm place amongst the instrumental methods of analysis. HPLC has caused a revolution in biological and pharmaceutical chemistry. Approximately two thirds of the publications on HPLC are concerned with problems from this area of life science. Biotechnology, where it is necessary to isolate substances from complicated mixtures, is likely to give further impetus to the dissemination of modern liquid chromatog­ raphy in columns, particularly on the preparative scale. This book presents, by means of examples, the application of HPLC to various fields, as well as fundamental discussions of chromatographic methods. The quality of the analytical result is decisively dependent on the qualities of the equipment employed (by Colin, Guiochon, and Martin). Especially the demands are discussed that are placed on the components of the instrument including those for data acquisition and processing. The section on "quantitative analy­ sis" (by ABhauer, Ullner) covers besides the principles also the problems of ensuring the quality of the data in detail. The basic problems arising by enlarging the sample size to preparative di­ mensions and the requirements put on the aparatus are discussed in the section on "preparative applications" (by Wehrli).

Editors and Affiliations

  • Angewandte Physikalische Chemie, Universität des Saarlandes, Saarbrücken, Germany

    Heinz Engelhardt

Bibliographic Information

  • Book Title: Practice of High Performance Liquid Chromatography

  • Book Subtitle: Applications, Equipment and Quantitative Analysis

  • Editors: Heinz Engelhardt

  • Series Title: Chemical Laboratory Practice

  • DOI: https://doi.org/10.1007/978-3-642-69225-3

  • Publisher: Springer Berlin, Heidelberg

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer-Verlag Berlin Heidelberg 1986

  • Softcover ISBN: 978-3-642-69227-7Published: 20 November 2013

  • eBook ISBN: 978-3-642-69225-3Published: 06 December 2012

  • Series ISSN: 0172-4967

  • Edition Number: 1

  • Number of Pages: XII, 464

  • Number of Illustrations: 13 b/w illustrations

  • Topics: Analytical Chemistry, Pharmacy, Biochemistry, general

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