T Cell Hybridomas

1. Front Matter

   Pages I-XI

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2. Lectin-Dependent Cytolytic and Cytolymic T Helper Clones and Hybridomas

   • K. Fischer Lindahl, A. A. Nordin, M. H. Schreier

   Pages 1-10

3. Cytotoxic T Cell Hybridomas: Generation and Characterization

   • Z. Eshhar, T. Waks, T. Oren, G. Berke, Y. Kaufmann

   Pages 11-18

4. Definition of Function-Related Isotypic Markers on T Cells

   • E. J. Culbert, S. Kontiainen, L. M. Douglas, M. Feldmann

   Pages 19-32

5. An Antigen-Specific Suppressor T Cell Factor Controlled by Two Genes in the Immunoglobulin Heavy Chain Linkage Group and in the I-J Subregion of the H-2 Complex

   • M. Taniguchi, T. Tokuhisa, M. Kanno, T. Honjo

   Pages 33-41

6. Structural and Functional Studies on Antigen-Specific Suppressor Factors from T Cells and T Cell Hybrids

   • M. J. Taussig, G. L. Asherson, A. Holliman, N. Richardson, G. Skibinski

   Pages 43-52

7. Purification and Biochemical Analysis of Antigen-Specific Suppressor Factors Isolated from T-Cell Hybridomas

   • D. R. Webb, B. A. Araneo, C. Healy, J. A. Kapp, K. Krupen, I. Nowowiejski et al.

   Pages 53-59

8. Dissection of a Suppressor Cell Cascade

   • M. E. Dorf, K. Okuda, M. Minami

   Pages 61-67

9. 4-Hydroxy-3-nitro-phenylacetyl (NP)-Specific T Cell Hybridomas

   • M. Cramer, R. Mierau, T. Takemori, G. Suzuki, T. Tada

   Pages 69-77

10. Regulation of the IgE Response by IgE Class-Specific Suppressor T Hybridomas

    • M. Suemura, K. Sugimura, A. Ishizaka, S. Kobatake, O. Shiho, H. Deguchi et al.

    Pages 79-88

11. Establishment of Functional, Antigen-Specific T Cell Lines by RadLV-Induced Transformation of Murine T Lymphocytes

    • P. Ricciardi-Castagnoli, F. Robbiati, E. Barbanti, G. Doria, L. Adorini

    Pages 89-96

12. H-2-Restricted Helper Hybridomas: One Locus or Two Control Dual Specificity?

    • P. Lonai, E. Arman, S. Bitton-Grossfeld, J. Grooten, G. Hämmerling

    Pages 97-102

13. T Cell Hybridomas Producing Antigen-Specific Factors Express Heavy-Chain Variable-Region Determinants

    • Z. Eshhar, T. Waks, H. Zinger, E. Mozes

    Pages 103-109

14. Cytochrome c Specific T Cell Hybrid

    • S. Carel, C. Bron, G. Corradin

    Pages 111-116

15. The Effect of Antigen Presentation on the Fine Specificity of Anti-Cytochrome c T Cell Hybridomas

    • E. Heber-Katz, D. Hansburg, R. H. Schwartz

    Pages 117-124

16. Analysis of the Anti-Self + TNP Immune Response: T Cell Lines, Clones and Hybridomas

    • A. M. Schmitt-Verhulst, M. A. Cooley, A. Guimezanes, C. De Préval, F. Albert, M. Buferne

    Pages 125-134

17. Functional Characteristics of T Cell Hybridomas Obtained by Fusion of TCGF-Dependent Helper T Cell Clones with BW5147

    • M. H. Schreier, R. Tees, L. Nordin, A. A. Nordin

    Pages 135-141

18. A Stable TCGF-Producing T Cell Hybridoma and its Thioguanine-Resistant Variant Suitable as a Tool for the Construction of New Functional T Hybridomas

    • K.-O. Grönvik, J. Andersson, F. Melchers, J. Zeuthen

    Pages 143-151

19. Influenza Virus-Specific Murine T Cell Hybridomas Which Recognize Virus Hemagglutinin in Conjunction with H-2d and Display Helper Functions for B Cells

    • F. Melchers, J. Zeuthen, W. Gerhard

    Pages 153-163

20. A “Panreactive” T Cell Line and T Cell Hybridoma: Their Function in Helping B Cells

    • J. Andersson, F. Melchers, J. Zeuthen

    Pages 165-172

For more than ten years cell fusion techniques have been applied in studies on various lymphocyte functions. Ig expression was first studied in hybrids obtained by fusing myeloma cells with fibroblasts (1) or lymphomas (2), both of which do not produce Ig, and with Ig­ producing myelomas (3) or human blood lymphocytes (4). Kohler and Milstein (5) fused a myeloma with spleen cells from immunized mice. Up to 10% of the hybrids obtained secreted antibodies specific for the immunizing antigen. This suggested that plasma cells preferenti­ ally fused with the myeloma cells, a finding which was of enormous practical value. It was found that both Band T lymphocytes could be fused with the T cell tumor BW5147, which is however not permissive for Ig synthesis (6). A very large number of T cell hybridomas were generated by fusing BW5147 with cell populations containing in vivo or in vitro activated cells (7). The hybrids showed no specific T cell functions and binding assays for T cell receptorswere not available. In particular, no hybrids were obtained which expreS1ed specific cytolytic activity that could be tested in short-term Cr­ release assays (8). However, the frustrations expressed about these failures, published in January, 1978 (9), were relieved by Taniguchi and Miller's publication a few months later of T cell hybridomas producing antigen-specific suppressor factors (10). Unfortunately, their hybrids rapidly lost factor production.

• Antigen
• T cell
• antibody
• blood
• cell
• lymphocytes
• lymphoma
• tumor

• Basel Institute for Immunology, Basel, Switzerland

  H. V. Boehmer, W. Haas, G. Köhler, F. Melchers, J. Zeuthen

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