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T Cell Hybridomas

A Workshop at the Basel Institute for Immunology

  • Conference proceedings
  • © 1982

Overview

Editors:
  1. H. V. Boehmer

    1. Basel Institute for Immunology, Basel, Switzerland

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  2. W. Haas

    1. Basel Institute for Immunology, Basel, Switzerland

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  3. G. Köhler

    1. Basel Institute for Immunology, Basel, Switzerland

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  4. F. Melchers

    1. Basel Institute for Immunology, Basel, Switzerland

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  5. J. Zeuthen

    1. Basel Institute for Immunology, Basel, Switzerland

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Part of the book series: Current Topics in Microbiology and Immunology (CT MICROBIOLOGY, volume 100)

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Table of contents (28 papers)

  1. Front Matter

    Pages I-XI
    Download chapter PDF

  2. Lectin-Dependent Cytolytic and Cytolymic T Helper Clones and Hybridomas

    • K. Fischer Lindahl, A. A. Nordin, M. H. Schreier
    Pages 1-10

  3. Cytotoxic T Cell Hybridomas: Generation and Characterization

    • Z. Eshhar, T. Waks, T. Oren, G. Berke, Y. Kaufmann
    Pages 11-18

  4. Definition of Function-Related Isotypic Markers on T Cells

    • E. J. Culbert, S. Kontiainen, L. M. Douglas, M. Feldmann
    Pages 19-32

  5. An Antigen-Specific Suppressor T Cell Factor Controlled by Two Genes in the Immunoglobulin Heavy Chain Linkage Group and in the I-J Subregion of the H-2 Complex

    • M. Taniguchi, T. Tokuhisa, M. Kanno, T. Honjo
    Pages 33-41

  6. Structural and Functional Studies on Antigen-Specific Suppressor Factors from T Cells and T Cell Hybrids

    • M. J. Taussig, G. L. Asherson, A. Holliman, N. Richardson, G. Skibinski
    Pages 43-52

  7. Purification and Biochemical Analysis of Antigen-Specific Suppressor Factors Isolated from T-Cell Hybridomas

    • D. R. Webb, B. A. Araneo, C. Healy, J. A. Kapp, K. Krupen, I. Nowowiejski et al.
    Pages 53-59

  8. Dissection of a Suppressor Cell Cascade

    • M. E. Dorf, K. Okuda, M. Minami
    Pages 61-67

  9. 4-Hydroxy-3-nitro-phenylacetyl (NP)-Specific T Cell Hybridomas

    • M. Cramer, R. Mierau, T. Takemori, G. Suzuki, T. Tada
    Pages 69-77

  10. Regulation of the IgE Response by IgE Class-Specific Suppressor T Hybridomas

    • M. Suemura, K. Sugimura, A. Ishizaka, S. Kobatake, O. Shiho, H. Deguchi et al.
    Pages 79-88

  11. Establishment of Functional, Antigen-Specific T Cell Lines by RadLV-Induced Transformation of Murine T Lymphocytes

    • P. Ricciardi-Castagnoli, F. Robbiati, E. Barbanti, G. Doria, L. Adorini
    Pages 89-96

  12. H-2-Restricted Helper Hybridomas: One Locus or Two Control Dual Specificity?

    • P. Lonai, E. Arman, S. Bitton-Grossfeld, J. Grooten, G. Hämmerling
    Pages 97-102

  13. T Cell Hybridomas Producing Antigen-Specific Factors Express Heavy-Chain Variable-Region Determinants

    • Z. Eshhar, T. Waks, H. Zinger, E. Mozes
    Pages 103-109

  14. Cytochrome c Specific T Cell Hybrid

    • S. Carel, C. Bron, G. Corradin
    Pages 111-116

  15. The Effect of Antigen Presentation on the Fine Specificity of Anti-Cytochrome c T Cell Hybridomas

    • E. Heber-Katz, D. Hansburg, R. H. Schwartz
    Pages 117-124

  16. Analysis of the Anti-Self + TNP Immune Response: T Cell Lines, Clones and Hybridomas

    • A. M. Schmitt-Verhulst, M. A. Cooley, A. Guimezanes, C. De Préval, F. Albert, M. Buferne
    Pages 125-134

  17. Functional Characteristics of T Cell Hybridomas Obtained by Fusion of TCGF-Dependent Helper T Cell Clones with BW5147

    • M. H. Schreier, R. Tees, L. Nordin, A. A. Nordin
    Pages 135-141

  18. A Stable TCGF-Producing T Cell Hybridoma and its Thioguanine-Resistant Variant Suitable as a Tool for the Construction of New Functional T Hybridomas

    • K.-O. Grönvik, J. Andersson, F. Melchers, J. Zeuthen
    Pages 143-151

  19. Influenza Virus-Specific Murine T Cell Hybridomas Which Recognize Virus Hemagglutinin in Conjunction with H-2d and Display Helper Functions for B Cells

    • F. Melchers, J. Zeuthen, W. Gerhard
    Pages 153-163

  20. A “Panreactive” T Cell Line and T Cell Hybridoma: Their Function in Helping B Cells

    • J. Andersson, F. Melchers, J. Zeuthen
    Pages 165-172
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Keywords

About this book

For more than ten years cell fusion techniques have been applied in studies on various lymphocyte functions. Ig expression was first studied in hybrids obtained by fusing myeloma cells with fibroblasts (1) or lymphomas (2), both of which do not produce Ig, and with Ig­ producing myelomas (3) or human blood lymphocytes (4). Kohler and Milstein (5) fused a myeloma with spleen cells from immunized mice. Up to 10% of the hybrids obtained secreted antibodies specific for the immunizing antigen. This suggested that plasma cells preferenti­ ally fused with the myeloma cells, a finding which was of enormous practical value. It was found that both Band T lymphocytes could be fused with the T cell tumor BW5147, which is however not permissive for Ig synthesis (6). A very large number of T cell hybridomas were generated by fusing BW5147 with cell populations containing in vivo or in vitro activated cells (7). The hybrids showed no specific T cell functions and binding assays for T cell receptorswere not available. In particular, no hybrids were obtained which expreS1ed specific cytolytic activity that could be tested in short-term Cr­ release assays (8). However, the frustrations expressed about these failures, published in January, 1978 (9), were relieved by Taniguchi and Miller's publication a few months later of T cell hybridomas producing antigen-specific suppressor factors (10). Unfortunately, their hybrids rapidly lost factor production.

Editors and Affiliations

  • Basel Institute for Immunology, Basel, Switzerland

    H. V. Boehmer, W. Haas, G. Köhler, F. Melchers, J. Zeuthen

Bibliographic Information

  • Book Title: T Cell Hybridomas

  • Book Subtitle: A Workshop at the Basel Institute for Immunology

  • Editors: H. V. Boehmer, W. Haas, G. Köhler, F. Melchers, J. Zeuthen

  • Series Title: Current Topics in Microbiology and Immunology

  • DOI: https://doi.org/10.1007/978-3-642-68586-6

  • Publisher: Springer Berlin, Heidelberg

  • eBook Packages: Springer Book Archive

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer-Verlag GmbH, DE, part of Springer Nature 1982

  • Softcover ISBN: 978-3-642-68588-0Published: 01 November 2011

  • eBook ISBN: 978-3-642-68586-6Published: 06 December 2012

  • Series ISSN: 0070-217X

  • Series E-ISSN: 2196-9965

  • Edition Number: 1

  • Number of Pages: XII, 264

  • Topics: Medical Microbiology, Oncology, Allergology, Immunology, Virology

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