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Membrane Biochemistry

A Laboratory Manual on Transport and Bioenergetics

  • Book
  • © 1979

Overview

Editors:
  1. Ernesto Carafoli

    1. Laboratory of Biochemistry, Swiss Federal Institute of Technology (ETH), Zurich, Switzerland

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  2. Giorgio Semenza

    1. Laboratory of Biochemistry, Swiss Federal Institute of Technology (ETH), Zurich, Switzerland

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Table of contents (17 chapters)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Nonelectrolyte Transport in Small Intestinal Membrane Vesicles. The Application of Filtration for Transport and Binding Studies

    • Markus Kessler, Gerhard Toggenburger
    Pages 1-24

  3. Transport of Sugars in Bacteria

    • Adam Kepes
    Pages 25-35

  4. Net Na+ and K+ Movements in Human Red Blood Cells After Cold Storage

    • Hans-Jürg Schatzmann, Peter Ronner, Verena Niggli
    Pages 36-40

  5. Calcium Transport in Resealed Erythrocytes and the Use of a Calcium-Sensitive Electrode

    • Peter Ronner
    Pages 41-50

  6. Calcium Transport in Sarcoplasmic Reticulum Vesicles Isolated from Rabbit Skeletal Muscle

    • Michele Chiesi, Anthony Martonosi
    Pages 51-61

  7. Preparation and Assay of Animal Mitochondria and Submitochondrial Vesicles

    • Paolo Gazzotti, Kerstin Malmström, Martin Crompton
    Pages 62-76

  8. Measurement of Cytochrome Kinetics in Rat Liver Mitochondria by Stopped Flow and Dual-Wavelength Spectrophotometry

    • Angelo Azzi, Hubert Affolter
    Pages 77-81

  9. Proton Translocation Catalyzed by Mitochondrial Cytochrome Oxidase

    • MÃ¥rten Wikström, Erwin Sigel
    Pages 82-91

  10. Determination of the Membrane Potential and pH Difference Across the Inner Mitochondrial Membrane

    • J. Brian Chappell, Martin Crompton
    Pages 92-97

  11. Anion Transport in Mitochondria

    • Martin Crompton, J. Brian Chappell
    Pages 98-102

  12. Calcium Transport in Mitochondria

    • Kerstin Malmström, Ernesto Carafoli
    Pages 103-112

  13. Redox Intermediates Between O2 and H2O

    • Christoph Richter
    Pages 113-119

  14. Photophosphorylation with Chromatophore Membranes from Rhodospirillum Rubrum

    • Kurt W. Hanselmann, Walter Beyeler, Christian Pflugshaupt, Reinhard Bachofen
    Pages 120-143

  15. Oxygen Evolution and Uptake as a Measure of the Light-Induced Electron Transport in Spinach Chloroplasts

    • Andres Binder, Reinhard Bachofen
    Pages 144-153

  16. The Function of the Purple Membrane in Halobacterium Halobium

    • Dieter Oesterhelt, Rainer Hartmann
    Pages 154-163

  17. Characterization of Ionophores Using Artificial Lipid Membranes

    • Michele Chiesi
    Pages 164-170

  18. Characterization of Neutral and Charged Ionophores Using Vesicular Artificial Lipid Membranes (Liposomes)

    • Paolo Gazzotti, Alec D. Bangham
    Pages 171-175
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Keywords

About this book

This manual collects in the form of laboratory protocols a series of experiments in the field of Membrane Transport and Membrane Bioenergetics. It represents the experience accumulated during four advanced courses held at the Depart­ ment of Biochemistry of the Swiss Federal Institute of Technology on behalf of Federation of European Biochemical Societies (FEBS) in the years 1975 through 1978. The idea of collecting the experiments into a laboratory manual developed as a response to a demand from the students who took part in the courses. Further motivation came with the fmding that, in planning the laboratory sessions, the teaching staff had no organized, modern source of information in the literature. The experiments presented cover most areas of importance in the subject mat­ ter. Their presentation has been continuously modified in the course of the four years during which the manual took shape, to accommodate to experience and various suggestions. In their present form, all of the experiments described have been repeatedly practiced to optimize their execution. Efforts have been made to combine in the manual classical experiments, and techniques which require relatively unsophisticated instrumentation and can therefore be carried out in most laboratories, with more modern experiments and relatively newer technol­ ogies. In its present form, the manual should therefore provide a usefui tool in the hands of researchers and laboratory teachers at different levels of sophisti­ cation and instrumentation.

Editors and Affiliations

  • Laboratory of Biochemistry, Swiss Federal Institute of Technology (ETH), Zurich, Switzerland

    Ernesto Carafoli, Giorgio Semenza

Bibliographic Information

  • Book Title: Membrane Biochemistry

  • Book Subtitle: A Laboratory Manual on Transport and Bioenergetics

  • Editors: Ernesto Carafoli, Giorgio Semenza

  • DOI: https://doi.org/10.1007/978-3-642-67530-0

  • Publisher: Springer Berlin, Heidelberg

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer-Verlag New York Inc. 1979

  • Softcover ISBN: 978-3-540-09844-7Published: 01 January 1980

  • eBook ISBN: 978-3-642-67530-0Published: 06 December 2012

  • Edition Number: 1

  • Number of Pages: X, 178

  • Number of Illustrations: 3 b/w illustrations

  • Topics: Biochemistry, general, Biomedicine general

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