Flow and Image Cytometry

1. Front Matter

   Pages I-VIII

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2. Membrane Dynamics and Function

   1. Front Matter

      Pages 1-1

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   2. Analysis of CD28 Interactions with Its Cognate Counter-Receptors CD80 and CD86

      • Alemseged Truneh, Manjula Reddy, Patricia Ryan, Sally D. Lyn, Ilona Kariv, Christopher Eichman et al.

      Pages 3-19

   3. Membrane Transport Dynamics

      • James V. Watson, Caroline Dive

      Pages 21-46

   4. Endogenous lectins in circulating cells and their glycosylated ligands: their role in T cell homing

      • Véronique Denis, Michèle Mitterand, Claudine Kieda

      Pages 47-51

   5. Use of Different Cytometric Techniques to Study the Cytotoxic Interaction between Human Natural Killer Cells and k562 Target cells

      • Katarina Radošević, Bart G. de Grooth, Jan Greve

      Pages 53-68

   6. In vitro Reconstitution of Early Endosome Membrane Dynamics

      • Jean-Pierre Gorvel, Zohair Mishal

      Pages 69-80

   7. Change in Membrane Fluidity after pinocytosis of a new Cytokine IK on Resting and Activated T cells

      • Raffaele Pereno, Patricia Krief, Zohar Mishal

      Pages 81-87

3. Cell Proliferation and Gene Expression

   1. Front Matter

      Pages 89-89

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   2. Cell Cycle and Cell Proliferation Markers

      • Harry A. Crissman, Anthony J. Nastasi

      Pages 91-101

   3. Advances in Flow Cytogenetics: Progress in the Development of a High Speed Optical Chromosome Sorter Based on Photoinduced Adduct Formation Between Psoralens and Chromosomal DNA

      • Mary C. Roslaniec, Richard J. Reynolds, John C. Martin, Ku Taek Han, L. Scott Cram

      Pages 103-114

   4. Analysis of Cell Death by Flow Cytometry

      • Zbigniew Darzynkiewicz, Xun Li

      Pages 115-130

   5. Molecular Cytogenetics: Uses of flow sorted chromosomes, fluorescence in situ hybridisation (FISH) and digital microscopy for the analysis of genomes

      • Nigel P. Carter

      Pages 131-141

   6. DNA Topoisomerases as Drug Targets and Cell Cycle Checkpoint Effector Molecules

      • Paul J. Smith, Nicola Blunt, Sylvie Souès

      Pages 143-153

   7. Hemopoietic Cell Differentiation and Death By Retinoids

      • Domenico Delia, Antonella Aiello, Marco A Pierotti

      Pages 155-165

4. Data Management Systems Cell Sorting Techniques Microscopy

   1. Front Matter

      Pages 167-167

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   2. Myc in the Control of Proliferation and Apoptosis

      • Lamorna Brown, Gerard Evan

      Pages 169-178

   3. Flow Cytometry: Analyses for All Sizes

      • James H. Jett

      Pages 179-190

   4. Flow Cytometric Immunophenotyping Using Cluster Analysis And Cluster Editing

      • Gary C. Salzman, Richard J. Beckman, Jim D. Parson, Aaron M. Nauman, Sigrid J. Stewart, Carleton C. Stewart

      Pages 191-212

   5. Introduction to High-Speed Flow Sorting

      • J. F. Keij

      Pages 213-227

Image analysis and flow cytometry are complementary techniques which provide new insights into essential aspects of cell biology. This book presents an up-to-date overview of current ideas and methods in these domains. It consists of three parts. Part I, "Membrane Dynamics and Function", deals with adhesion molecules, membrane pump dynamics, membrane fusion and endocytosis, membrane potential, T-cell homing and cytoskeleton, etc. The second part focuses on problems of cell proliferation, chromosome analysis and sorting, apoptosis, imaging cytometry of fixed and living cells, image analysis for gene mapping from flow-sorted chromosomes, chromatin organization, and liposome-mediated delivery of DNA binding drugs. The third part covers data management systems, cell sorting techniques and microscopy.

• DNA
• Zytometrie
• apoptosis
• biology
• cell
• cell biology
• chromosome
• cytokine
• flow cytometry
• gene
• gene expression
• genetics
• membrane
• membrane transport
• microscopy

• Centre National de le Recherche Scientifique, Laboratorie de Cytométrie (UPS 47), Villejuif, France

  Alain Jacquamin-Sablon

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