Overview
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Table of contents (25 chapters)
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Front Matter
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Template Generation for Cell-Free Protein Expression Systems
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Front Matter
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Labeling Proteins in Cell-Free Protein Expression Systems for Structural and Functional Analysis
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Front Matter
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Screening and Increasing Soluble Expression of Recombinant Proteins
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Front Matter
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Keywords
About this book
Cell-free protein synthesis is coming of age! Motivated by an escalating need for efficient protein synthesis and empowered by readily accessible cell-free protein synthesis kits, the technology is expanding both in the range of feasible proteins and in the ways that proteins can be labeled and modified. This volume follows "Cell-Free Translation Systems", edited by Professor Alexander S. Spirin in 2002. Since then, an impressive collection of new work has emerged that demonstrates a substantial expansion of capability. In this volume, we show that proteins now can be efficiently produced using PCR products as DNA templates and that even membrane proteins and proteins with multiple disulfide proteins are obtained at high yields. Many additional advances are also presented. It is an exciting time for protein synthesis technology.
Editors and Affiliations
Bibliographic Information
Book Title: Cell-Free Protein Expression
Editors: James R. Swartz
DOI: https://doi.org/10.1007/978-3-642-59337-6
Publisher: Springer Berlin, Heidelberg
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eBook Packages: Springer Book Archive
Copyright Information: Springer-Verlag Berlin Heidelberg 2003
Softcover ISBN: 978-3-642-63939-5Published: 19 September 2011
eBook ISBN: 978-3-642-59337-6Published: 06 December 2012
Edition Number: 1
Number of Pages: X, 230
Number of Illustrations: 40 illustrations in colour
Topics: Biochemistry, general