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Fluorescence Spectroscopy, Imaging and Probes

New Tools in Chemical, Physical and Life Sciences

  • Book
  • © 2002

Overview

Editors:
  1. Ruud Kraayenhof

    1. Institute of Molecular Biological Sciences, Amsterdam, Vrije Universiteit, Amsterdam, The Netherlands

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  2. Antonie J. W. G. Visser

    1. MicroSpectroscopy Center, Wageningen University, Wageningen, The Netherlands

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    You can also search for this editor in PubMed   Google Scholar

  3. Hans C. Gerritsen

    1. Dept. of Molecular Biophysics, University of Utrecht, Utrecht, The Netherlands

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    You can also search for this editor in PubMed   Google Scholar

  • Volume 2 of this new series focuses on biomedical and physico-chemical research and application
  • Many frontline researcher have contributed to this highly attractive and interdisciplinary volume
  • Includes supplementary material: sn.pub/extras

Part of the book series: Springer Series on Fluorescence (SS FLUOR, volume 2)

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Table of contents (23 chapters)

  1. Front Matter

    Pages I-XXV
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  2. Fluorescence Spectroscopy: New Approaches and Probes

    1. Front Matter

      Pages 1-1
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    2. Advanced Luminescent Labels, Probes and Beads and their Application to Luminescence Bioassay and Imaging

      • O. S. Wolfbeis, M. Böhmer, A. Dürkop, J. Enderlein, M. Gruber, I. Klimant et al.
      Pages 3-42

    3. Fluorescence Spectral Engineering — Biophysical and Biomedical Applications

      • J. R. Lakowicz, I. Gryczynski, Y. Shen, J. Malicka, S. D’Auria, Z. Gryczynski
      Pages 43-68

    4. Fluorescence Nanometrology in Sol-Gels

      • D. J. S. Birch, C. D. Geddes, J. Karolin, R. Leishman, O. J. Rolinski
      Pages 69-85

    5. Integrated Supramolecular Systems: From Sensors to Switches

      • J.-P. Malval, I. Gosse, J.-P. Morand, R. Lapouyade
      Pages 87-100

    6. Ratiometric Probes: Design and Applications

      • A. P. Demchenko, A. S. Klymchenko, V. G. Pivovarenko, S. Ercelen
      Pages 101-110

    7. Binding of Ethidium to Yeast tRNAPhe: A New Perspective on an Old Bromide

      • M. Tramier, O. Holub, J. C. Croney, T. Ishi, S. E. Seifried, D. M. Jameson
      Pages 111-121

    8. Experimental Aspects of DNA Computing by Blocking: Use of Fluorescence Techniques for Detection

      • K. A. Schmidt, C. v. Henkel, G. Rozenberg, H. P. Spaink
      Pages 123-128

  3. Fluorescence Spectroscopy of Single Molecules and Molecular Assemblies

    1. Front Matter

      Pages 129-129
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    2. Multiparametric Detection of Fluorescence Emitted from Individual Multichromophoric Systems

      • M. Cotlet, J. Hofkens, M. Maus, F. C. de Schryver
      Pages 131-151

    3. Fluorescence Intensity Distribution Analysis (FIDA) and related fluorescence fluctuation techniques: theory and practice

      • P. Kask, C. Eggeling, K. Palo, Ãœ. Mets, M. Cole, K. Gall
      Pages 153-181

    4. Single Molecule Reactions of the Enzyme LDH and of Restriction Endonucleases in the Fluorescence Microscope

      • B. Nasanshargal, B. Schäfer, K. O. Greulich
      Pages 183-195

    5. Monitoring γ-Subunit Movement in Reconstituted Single EF°F1 ATP Synthase by Fluorescence Resonance Energy Transfer

      • M. Börsch, M. Diez, B. Zimmermann, R. Reuter, P. Gräber
      Pages 197-207

  4. Application of Fluorescence in Biological Membrane and Enzyme Studies

    1. Front Matter

      Pages 209-209
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    2. Application of the Wavelength-selective Fluorescence Approach to Monitor Membrane Organization and Dynamics

      • A. Chattopadhyay
      Pages 211-224

    3. Fluorescence Approaches for the Characterization of the Peripheral Membrane Binding of Proteins Applied for the Blood Coagulation Protein Prothrombin

      • R. Hutterer, M. Hof
      Pages 225-240

    4. Assessment of Membrane Fluidity in Individual Yeast Cells by Laurdan Generalised Polarisation and Multi-photon Scanning Fluorescence Microscopy

      • R. P. Learmonth, E. Gratton
      Pages 241-252

    5. Formation of Higher Order Signal Transduction Complexes as Seen by Fluorescence Spectroscopy

      • L. Dowal, S. Scarlata
      Pages 253-261

    6. Mechanisms of the Modulation of Membrane Interfacial Enzyme Catalysis by Non-lamellar Forming Lipids: Comparison with the Behavior of a Fluorescent Probe in Membranes

      • R. M. Epand, R. Cornell, S. M. A. Davies, R. Kraayenhof
      Pages 263-276
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Keywords

About this book

The application of fluorescence in drug discovery, high-throughout screenings in genomics and proteomics is and will be evidently successful. The increased use of fluorescence techniques is greatly enhanced by the improved instrumentation pioneered by inventive scientists and now made available commercially by several high-tech companies. Moreover, the design and development of many new molecular probes with higher selectivity for specific microenvironmental properties has stimulated many new researchers to employ fluorescence techniques for solving their problems. Probably the most significant breakthrough in fluorescence is its use in detection of single molecules and even of their real-time dynamics. Also, probing inside living cells has become a hot topic in the life sciences. This topic book reflects the updates of scientific progress presented by frontline researchers.

Editors and Affiliations

  • Institute of Molecular Biological Sciences, Amsterdam, Vrije Universiteit, Amsterdam, The Netherlands

    Ruud Kraayenhof

  • MicroSpectroscopy Center, Wageningen University, Wageningen, The Netherlands

    Antonie J. W. G. Visser

  • Dept. of Molecular Biophysics, University of Utrecht, Utrecht, The Netherlands

    Hans C. Gerritsen

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