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Rapid Cycle Real-Time PCR — Methods and Applications

Quantification

  • Book
  • © 2004

Overview

Editors:
  1. Carl Wittwer

    1. Department of Pathology, University of Utah, School of Medicine, Salt Lake City, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Meinhard Hahn

    1. Deutsches Krebsforschungszentrum, Abt. Molekulare Genetik, Heidelberg, Germany

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    You can also search for this editor in PubMed   Google Scholar

  3. Karen Kaul

    1. Department of Pathology, Evanston Northwestern Healthcare, Evanston, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

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Table of contents (21 chapters)

  1. Front Matter

    Pages I-VII
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  2. Methods

    1. Front Matter

      Pages 1-1
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    2. Methods

      1. Housekeeping Genes: A Gold Standard?
        • Onno Bakker, Daphne C. Timmer
        Pages 3-10
      2. The Choice of House Keeping Genes in MRD-Quantification of AML1-ETO Positive Acute Myeloid Leukemia
        • Martin Weisser, Claudia Schoch, Torsten Haferlach, Wolfgang Hiddemann, Susanne Schnittger
        Pages 11-20
      3. Quantification of mRNA Using Linear Regression of Log-Linear PCR Data-Points as an Alternative for the Standard Curve Approach
        • Christian Ramakers, Daphne Timmer, Onno Bakker, Ronald H. Lekanne Deprez, Jan M. Ruijter, Antoon F. M. Moorman
        Pages 21-29
      4. Measuring Genome Sizes by Absolute Quantification
        • Jochen Wilhelm, Meinhard Hahn
        Pages 31-41

  3. Applications

    1. Front Matter

      Pages 43-43
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    2. Regulation and Development

      1. Relative Quantification of Insulin Gene Expression on the LightCycler Using SYBR Green I
        • Nicole Neubauer
        Pages 45-51
      2. Quantification of δRec-ψJα Signal Joint T-Cell Receptor Excision Circle DNA in Patients after Autologous and Allogeneic Stem Cell Transplantation
        • Juergen Loeffler, Holger Hebart, Lutz Lochmann, Thomas Daikeler, Peter Bader, Ralf Bauer et al.
        Pages 53-59
      3. Expression Analysis of Mitochondrial Components in a Variety of Plant Species Using Real-Time Quantitative PCR
        • Katharine A. Howell, Ryan Lister, James Whelan
        Pages 61-72
      4. Quantification of Ikaros Splice Variants by Real-Time PCR
        • Elli Veistinen, Kalle-Pekka Nera, Jukka Alinikula, Olli Lassila
        Pages 73-82
      5. Methods to Quantify Cytokine Gene Expression by Real-Time PCR
        • Patrick Stordeur
        Pages 83-92

    3. Oncology

      1. Profiling Breast Cancer Using Real-Time Quantitative PCR
        • Scot G. Frank, Philip S. Bernard
        Pages 95-106
      2. <i>HER-2/neu</i> Gene Copy Number Quantified by Real-Time PCR in Cell Lines and Breast Cancer Tissue
        • Melanie Königshoff, Jochen Wilhelm, Meinhard Hahn
        Pages 107-116
      3. Quantification of Several Components of Fibrinolytic and Matrix Metalloproteinase Systems in Primary Breast Cancer
        • Remedios Castelló, Francisco España, Justo Aznar, Amparo Estellés
        Pages 117-124
      4. A Rapid Cycle Real-Time Quantitative β-globin PCR for Quantification of Human DNA in Feces
        • Corné H.W. Klaassen, Clemens F.M. Prinsen, Frederik B.J.M. Thunnissen
        Pages 125-130
      5. Quantification of Tumor Load for Follicular Lymphoma by Quantifying <i>BCL2/IGH</i> Using Real-Time Quantitative PCR by LightCycler™
        • Chung-Che Chang, Bernard C. Schur
        Pages 131-140
      6. Real-Time Quantification of the <i>AML</i> Rearrangements, <i>AML1-ETO</i> and <i>TEL-AML1</i>, in Acute Leukemia
        • Eva Barragan, Pascual Bolufer, Miguel Angel Sanz
        Pages 141-155

    4. Genetics

      1. Rapid Detection of Gene Duplications in Charcot-Marie-Tooth 1A Disease by SNP Genotyping Using Real-Time PCR
        • C. Ruiz-Ponte, A. Vega, L. Loidi, A. Carracedo, F Barros
        Pages 159-169
      2. Cytochrome P450 2D6 Deletion Genotyping Using Derivative Curve Analysis on the LightCycler
        • Alison Millson, Elizabeth L. Frank, Elaine Lyon
        Pages 171-178
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Keywords

About this book

Rapid cycle real-time PCR is a powerful technique for nucleic acid quantification and analysis that takes less than 30 minutes to complete. Fluorescence is automatically monitored each cycle and the amount of template quantified by advanced analytical methods, such as the second derivative maximum method. Immediately following rapid cycle PCR, melting curve analysis is performed to verify product purity with SYBR Green I and/or genotype with fluorescently-labeled hybridization probes (HybProbes or SimpleProbes). Rapid cycle real-time PCR is often cited as the most versatile, efficient method for nucleic acid quantification in research and climical studies. Molecular analysis has never been easier!

Editors and Affiliations

  • Department of Pathology, University of Utah, School of Medicine, Salt Lake City, USA

    Carl Wittwer

  • Deutsches Krebsforschungszentrum, Abt. Molekulare Genetik, Heidelberg, Germany

    Meinhard Hahn

  • Department of Pathology, Evanston Northwestern Healthcare, Evanston, USA

    Karen Kaul

Bibliographic Information

  • Book Title: Rapid Cycle Real-Time PCR — Methods and Applications

  • Book Subtitle: Quantification

  • Editors: Carl Wittwer, Meinhard Hahn, Karen Kaul

  • DOI: https://doi.org/10.1007/978-3-642-18840-4

  • Publisher: Springer Berlin, Heidelberg

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer-Verlag Berlin Heidelberg 2004

  • Hardcover ISBN: 978-3-540-20629-3Published: 16 December 2003

  • Softcover ISBN: 978-3-642-62317-2Published: 23 October 2012

  • eBook ISBN: 978-3-642-18840-4Published: 06 December 2012

  • Edition Number: 1

  • Number of Pages: VII, 223

  • Number of Illustrations: 15 b/w illustrations, 511 illustrations in colour

  • Topics: Biochemistry, general

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