Microsurgical Models in Rats for Transplantation Research

1. Front Matter

   Pages I-XIX

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2. Microsurgical Models and Techniques

   1. History and Development of Experimental Microsurgery in Rats

      • Sun Lee

      Pages 1-10

   2. Reflections on the Requirements and Perspectives of a Microsurgical Research Group

      • A. Thiede, E. Deltz, R. Engemann

      Pages 11-18

   3. Techniques of Microsurgical Vascular Anastomoses in Experimental Microsurgery

      • R. Engemann

      Pages 19-24

   4. Heart Transplantation in Rats

      • W. Konertz, M. Semik, A. Bernhard

      Pages 25-30

   5. Accessory Heart Transplantation in the Neck: A Simple Model in the Rat Using the Cuff Technique for Vascular Anastomoses

      • W. Timmermann

      Pages 31-35

   6. Microvascular Pulmonary Transplantation in Rats

      • W. Konertz

      Pages 37-42

   7. Spleen and Vascularized Skin Flap Transplantation

      • G. H. Müller, A. Wunderlich

      Pages 43-46

   8. Renal Transplantation in the Rat

      • H. D. Jakubowski

      Pages 47-50

   9. Microsurgical Technique for Small-Intestine Transplantation

      • E. Deltz, A. Thiede

      Pages 51-55

   10. Various Techniques of Experimental Pancreas Transplantation in the Rat

       • T. Schang, W. Timmermann, A. Thiede

       Pages 57-63

   11. Experiences in Rat Pancreatic Islet-Cell Isolation and Transplantation

       • K. Ulrichs, W. Timmermann, T. Schang

       Pages 65-68

   12. Technique for Orthotopic Rat Liver Transplantation

       • R. Engemann

       Pages 69-75

3. Immunological Principles

   1. Theoretical Aspects of Rat Organ Transplantation

      • W. Müller-Ruchholtz

      Pages 77-81

   2. Immunogenetic Aspects of Organ Transplantaion in the Rat

      • E. Günther

      Pages 83-94

4. Results of Vessel and Heart Transplantation

   1. Venovenous Allotransplantation in the Rat

      • T. Schang, B. von Gaudecker, A. Thiede

      Pages 95-100

   2. The Rejection Response to Segmental Grafts of the Femoral Artery in Inbred Rat Strains

      • W. Timmermann, A. Thiede

      Pages 101-105

   3. Factors Influencing Endothelial-Cell Viability During Procurement and Preservation of Valve Allografts

      • A. C. Yankah, G. Randzio, H. U. Wottge, A. Bernhard

      Pages 107-111

   4. The Rat Model as Precursor of Clinical Lymph Vessel Transplantation

      • R. G. H. Baumeister, J. Seifert, H. Liebich, B. Wiebecke, Ch. Gabka, U. Goldmann

      Pages 113-116

   5. Microstructure of Allogeneic Lymph Vessel Transplants in the Rat

      • Ch. Gabka, R. G. Baumeister, H. G. Leibich, J. Seifert, W. Brendel

      Pages 117-120

Preclinical experimental transplantation research that is based on microsurgical models in rats fulfills two indispensable conditions for modern organ transplanta­ tion research: Almost all organ grafts can be performed on the rat with an amount of technical effort that is still justifiable. Thus transplantation models that are analogous to human organ transplantation can be developed, tested, and evaluated. This fulfills a necessary condition from the standpoint of surgery. With the species rat, we have a great variety of genetically different inbred strains. From the immunological point of view this is an indispensable prerequisite for the investigation of preclinical transplantation models that can be expected to produce controllable, reproducible results. In vivo experimental results can be supplemented by and correlated to in vitro tests. Lately these experimental results are being greatly expanded and more precisely defined by the application of immunohistological methods that have been established recently in Kiel. In this book we hope to present a cross section of the microsurgical models in use today and of current immunological and immunohistological models. Furthermore, we wish to record the present state of microsurgical organ transplantation research and to show its relationship to the current state and development of clinical organ transplantation. A special aspect of our Kiel research group is the long-term, well-functioning, interdisciplinary cooperation between surgery, immunology, and pathology. Through this cooperation we attempt to provide an atmosphere in which theoretical and practical viewpoints can mutually influence each other.

• development
• histology
• immunology
• microsurgery
• organ
• organ transplantation
• pathology
• reconstructive surgery
• research
• surgery
• transplantation

• Abteilung Allgemeine Chirurgie, Chirurgische Universitätsklinik, Kiel 1, Federal Republic of Germany

  Arnulf Thiede, Eberhard Deltz, Rainer Engemann, Horst Hamelmann

Policies and ethics