Skip to main content
SpringerLink
Log in

Fluorescence Correlation Spectroscopy

Theory and Applications

  • Book
  • © 2001

Overview

Authors:
  1. Rudolf Rigler

    1. Department of Medical Biophysics, Karolinska Institutet, Stockholm, Sweden

    View author publications

    You can also search for this author in PubMed   Google Scholar

  2. Elliot S. Elson

    1. Department of Biochemistry and Molecular Biophysics, School of Medicine, Washington University, St. Louis, USA

    View author publications

    You can also search for this author in PubMed   Google Scholar

  • First book on this important analytical technique

Part of the book series: Springer Series in Chemical Physics (CHEMICAL, volume 65)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 39.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (22 chapters)

  1. Front Matter

    Pages I-XX
    Download chapter PDF

  2. 1 Introduction

    1. Introduction

      • E. Elson
      Pages 1-6

  3. FCS in the Analysis of Molecular Interactions

    1. Front Matter

      Pages 7-7
      Download chapter PDF

    2. Fluorescence Correlation Spectroscopy of Flavins and Flavoproteins

      • Antonie J. W. G. Visser, Petra A. W. van den Berg, Mark A. Hink, Valentin N. Petushkov
      Pages 9-24

    3. Fluorescence Correlation Spectroscopy in Nucleic Acid Analysis

      • Zeno Foldes-Pappn, Masataka Kinjo
      Pages 25-64

    4. Strain-Dependent Fluorescence Correlation Spectroscopy: Proposing a New Measurement for Conformational Fluctuations of Biological Macromolecules

      • Hong Qian, Elliot L. Elson
      Pages 65-83

    5. Applications of FCS to Protein-Ligand Interactions: Comparison with Fluorescence Polarization

      • Edmund Matayoshi, Kerry Swift
      Pages 84-98

  4. FCS at the Cellular Level

    1. Front Matter

      Pages 99-99
      Download chapter PDF

    2. FCS-Analysis of Ligand-Receptor Interactions in Living Cells

      • Alladin Pramanik, Rudolf Rigler
      Pages 101-131

    3. Fluorescence Correlation Microscopy (FCM): Fluorescence Correlation Spectroscopy (FCS) in Cell Biology

      • Roland Brock, Thomas M. Jovin
      Pages 132-161

    4. FCS and Spatial Correlations on Biological Surfaces

      • Nils O. Petersen
      Pages 162-184

  5. Applications in Biotechnology, Drug Screening, and Diagnostics

    1. Front Matter

      Pages 185-185
      Download chapter PDF

    2. Dual-Color Confocal Fluorescence Spectroscopy and its Application in Biotechnology

      • Andre Koltermann, Ulrich Kettling, Jens Stephan, Thorsten Winkler, Manfred Eigen
      Pages 187-203

    3. Nanoparticle Immunoassays: A new Method for Use in Molecular Diagnostics and High Throughput Pharmaceutical Screening based on Fluorescence Correlation Spectroscopy

      • F. J. Meyer-Almes
      Pages 204-224

    4. Protein Aggregation Associated with alzhiemer and Prion Diseases

      • Detlev Riesner
      Pages 225-247

  6. Environmental Analysis and Monitoring

    1. Front Matter

      Pages 249-249
      Download chapter PDF

    2. Application of FCS to the Study of Environmental Systems

      • Konstantin Starchev, Kevin Wilkinson, Jacques Buffle
      Pages 251-275

    3. Photophysical Aspects of FCS Measurements

      • Jerker Widengren
      Pages 276-301

  7. Environmental Analysis and Monitoring

    1. Front Matter

      Pages 303-303
      Download chapter PDF

    2. Fluorescence Correlation Spectroscopy: Genesis, Evolution, Maturation and Prognosis

      • Watt W. Webb
      Pages 305-330
Back to top

Keywords

About this book

Fluorescence correlation spectroscopy (FCS) was developed in order to char­ acterize the dynamics of molecular processes in systems in thermodynamic equilibrium. FCS determines transport and chemical reaction rates from mea­ surements of spontaneous microscopic thermally driven molecular concentra­ tion fluctuations. Since its inception, and particularly in recent years, techni­ cal and conceptual advances have extended the range of practical applicability and the information obtainable from FCS measurements. Improvements in microscopy, data acquisition, and data processing have greatly shortened the time required for FCS measurements. FCS can now be routinely applied to labile systems such as cells, and for the acquisition of large volumes of data as required for high-throughput screening. Cross correlation methods pro­ vide a powerful tool for characterizing interactions among different molecular species. Analysis of the amplitude of concentration fluctuations can provide a wealth of information about aggregation/polymerization process and the compositions of mixtures. Furthermore, FCS provides a bridge between conventional measurements of dynamic processes on a macroscopic concentration scale and the currently developing field of single molecule measurements. Both FCS and single mole­ cule approaches measure directly stochastic fluctuations in molecular pro­ perties, and so must be analyzed by statistical methods to yield conventional phenomenological parameters. As commonly practiced, FCS yields these phe­ nomenological parameters, e. g. , diffusion coefficients and chemical rate con­ stants, directly in terms of a fluorescence fluctuation autocorrelation func­ tion.

Authors and Affiliations

  • Department of Medical Biophysics, Karolinska Institutet, Stockholm, Sweden

    Rudolf Rigler

  • Department of Biochemistry and Molecular Biophysics, School of Medicine, Washington University, St. Louis, USA

    Elliot S. Elson

Bibliographic Information

Publish with us

Policies and ethics

Back to top

Search

Navigation