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Rapid Cycle Real-Time PCR — Methods and Applications

Genetics and Oncology

  • Book
  • © 2002

Overview

Editors:
  1. Wolfgang Dietmaier

    1. Institut für Pathologie, Regensburg, Germany

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    You can also search for this editor in PubMed   Google Scholar

  2. Carl Wittwer

    1. Department of Pathology, University of Utah Medical School, Salt Lake City, USA

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    You can also search for this editor in PubMed   Google Scholar

  3. Natarajan Sivasubramanian

    1. Baylor College of Medicine, Houston, USA

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    You can also search for this editor in PubMed   Google Scholar

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Table of contents (21 chapters)

  1. Front Matter

    Pages I-VIII
    Download chapter PDF

  2. Introduction for Genetics and Oncology Volume Rapid Cycle Real-Time PCR Methods and Applications

    1. Introduction for Genetics and Oncology Volume Rapid Cycle Real-Time PCR Methods and Applications

      • Wolfgang Dietmaier, Carl Wittwer, Natarajan Sivasubramanian
      Pages 1-2

  3. Methods Useful in Genetics and Oncology

    1. Front Matter

      Pages 3-3
      Download chapter PDF

    2. Quantification of Cytokine mRNAs in Human Myocardial Biopsy Samples by Real-Time Quantitative PCR Technology Using the LightCycler Instrument

      • Xi Zhu, Georg Baumgarten, Feng Wang, Ziad Dibbs, Abhinav Diwan, Guillermo Torre-Amione et al.
      Pages 5-14

    3. Quantitative Two-Step RT-PCR for the Detection of Human ABCA1 Transporter on LightCycler Using Hybridization Probes and External Standards

      • Danuta Kielar, Wolfgang Dietmaier, Thomas Langmann, Charalampos Aslanidis, Mario Probst, Marek Naruszewicz et al.
      Pages 15-25

    4. Quantification of Human Genomic DNA Using Retinoic X Receptor B Gene

      • Nathalie Pieri-Balandraud, Jean Roudier, Chantal Roudier
      Pages 27-33

    5. Genotyping by Guanosine-Dependent Quenching of Single-Labeled Fluorescein Probes

      • Andrew O. Crockett
      Pages 35-46

    6. Limitations of Melting Curve Analysis Using SYBR Green I — Fragment Differentiation and Mutation Detection in the CFTR-Gene

      • S. Kleinle, K. Tabiti, S. Gallati
      Pages 47-56

    7. SYBR Green I Analysis of the Trinucleotide Repeat Responsible for Huntington’s Disease

      • Cameron N. Gundry, Carl T. Wittwer
      Pages 57-63

  4. Applications in Genetics

    1. Front Matter

      Pages 65-65
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    2. Parallel Genotyping of Different Genes: A Rapid Real-Time PCR Approach

      • Stefan Fronhoffs, Thomas Brüning, Hans Vetter, Yon Ko
      Pages 67-75

    3. Detection of a Single Base Substitution in Single Cells by Melting Peak Analysis Using Dual-Color Hybridization Probes

      • Gerard Pals
      Pages 77-84

    4. Rapid Screening for Five Major Cystic Fibrosis Mutations by Melting Peak Analysis Using Fluorogenic Hybridization Probes

      • Siegfried Burggraf, Naeem Malik, Edith Schuhmacher, Bernhard Olgemöller
      Pages 85-94

    5. LightCycler PCR for the Polymorphisms -308 and -238 in the TNF Alpha Gene and for the TNFB1/B2 Polymorphism in the LT Alpha Gene

      • Lukas Bestmann, Näder Helmy, Felicia Garofalo, Aynur Demirtas, Dieter Vonderschmitt, Friedrich E. Maly
      Pages 95-105

    6. Rapid Genotyping of 2-bp and 9-bp Deletion Mutations Using the LightCycler Instrument

      • Tsutomu Aoshima, Mitsuharu Kajita, Yoshitaka Sekido, Shunji Mimura, Kazuyoshi Watanabe, Kaoru Shimokata et al.
      Pages 107-114

    7. Genotyping of the Methionine-Valine Polymorphism at Codon 129 of the Human Prion Protein by Melting Point Analysis of Fluorescently Labeled Hybridization Probes

      • Siegfried Burggraf, Siegfried Kösel, Sabine Lohmann, Reinhard Beck, Bernhard Olgemöller
      Pages 115-127

    8. Rapid Detection of Missense Mutations in the Prostatic Steroid 5α-Reductase Gene Using Real-Time Fluorescence PCR and Melting Curve Analysis

      • Markus Nauck, Winfried März, Heinrich Wieland
      Pages 129-135

  5. Applications in Oncology

    1. Front Matter

      Pages 137-137
      Download chapter PDF

    2. Analysis of Microsatellite Instability by Melting Peak Analysis with BAT26 and BAT25 Specific Fluorescence Hybridization Probes

      • Wolfgang Dietmaier, Arndt Hartmann, Ferdinand Hofstädter
      Pages 139-146

    3. Two Color Multiplexing and Typing of Human Papillomavirus Types 16,18 and 45 on LightCycler

      • Monica L. Henriquez, Brian E. Caplin, Randy P. Rasmussen
      Pages 147-157
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Keywords

About this book

Rapid-Cycle Real-Time PCR is a powerful technique for nucleic acid amplification and analysis that often requires less than half an hour to perform. Samples are amplified by rapid-cycle PCR followed by immediate melting curve analysis in the same instrument. Melting curve analysis of PCR products with SYBR Green I often allows product identification without gel electrophoresis. Furthermore, in the presence of fluorescent hybridization probes, melting curves provide "dynamic dot blots" for fine sequence analysis, including single nucleotide polymorphisms (SNPs). The method is often cited as the most versatile, efficient method for nucleic acid analysis in research and diagnostics in the fields of genetics and oncology. Molecular diagnostics has never been easier!

Editors and Affiliations

  • Institut für Pathologie, Regensburg, Germany

    Wolfgang Dietmaier

  • Department of Pathology, University of Utah Medical School, Salt Lake City, USA

    Carl Wittwer

  • Baylor College of Medicine, Houston, USA

    Natarajan Sivasubramanian

Bibliographic Information

  • Book Title: Rapid Cycle Real-Time PCR — Methods and Applications

  • Book Subtitle: Genetics and Oncology

  • Editors: Wolfgang Dietmaier, Carl Wittwer, Natarajan Sivasubramanian

  • DOI: https://doi.org/10.1007/978-3-642-59397-0

  • Publisher: Springer Berlin, Heidelberg

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer-Verlag Berlin Heidelberg 2002

  • Softcover ISBN: 978-3-642-63965-4Published: 30 September 2011

  • eBook ISBN: 978-3-642-59397-0Published: 29 June 2013

  • Edition Number: 1

  • Number of Pages: VIII, 205

  • Number of Illustrations: 11 b/w illustrations, 528 illustrations in colour

  • Topics: Oncology, Molecular Medicine

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