Cell Cycle - Materials and Methods

1. Front Matter

   Pages I-XXX

   PDF

2. Techniques for Studying the Mammalian Cell Cycle

   1. Front Matter

      Pages 1-1

      PDF

   2. Use of Immunohistochemistry in Cell Cycle Studies

      • Jirina Bartkova, Jìri Lukas, Jìri Bartek

      Pages 3-14

   3. Immunofluorescence Techniques in Cell Cycle Studies

      • M. Cristina Cardoso, Heinrich Leonhardt

      Pages 15-28

   4. Fluorescence in Situ Hybridization and Human Cell Cycle Genes

      • Douglas J. Demetrick

      Pages 29-45

   5. Techniques for Monitoring Cell Cycle Phases

      • Marina Giunta, Carlo Pucillo

      Pages 46-52

   6. Centrifugal Elutriation

      • Robert L. Marraccino, Peter C. Keng

      Pages 53-62

   7. Synchronization of Mammalian Cells

      • Patrick M. O’Connor, Joany Jackman

      Pages 63-74

   8. Microinjection and Electroporation of Macromolecules into Live Cells

      • Rainer Pepperkok

      Pages 75-86

   9. Stable and Transient Transfections in Cell Cycle Studies

      • Mark Rolfe

      Pages 87-92

   10. Quantification of Premature and Normal Mitosis

       • Sun W. Tam, Robert Schlegel

       Pages 93-99

3. Techniques for Studying the Xenopus Cell Cycle

   1. Front Matter

      Pages 101-101

      PDF

   2. Preparation of Xenopus Egg Extracts and Their Utilization in Cell Cycle Studies

      • Paul R. Clarke

      Pages 103-116

4. Techniques for Study the Cell Cycle

   1. Front Matter

      Pages 117-117

      PDF

   2. Cloning and Expression of Cell Cycle Genes in Schizosaccharomyces pombe

      • Guillaume Cottarel

      Pages 119-131

   3. Preparation of Synchronous Cultures of the Fission yeast, Schizosaccharomyces pombe

      • James Creanor

      Pages 132-142

5. Techniques for Studying the Cell Cycle of Any Species

   1. Front Matter

      Pages 143-143

      PDF

   2. Identification of Novel Cell Cycle Targets Using Small Molecule Ligands

      • Vivian Berlin, M. Isabel Chiu

      Pages 145-156

   3. Bacterial Expression and in Vitro Assay of Cell Cycle Kinases

      • Lisa Connell-Crowley, Wade Harper

      Pages 157-168

   4. Heterologous Expression and Secretion of Cell Cycle Proteins Using the Yeast Picchia pastoris

      • Guillaume Cottarel

      Pages 169-174

During their lifetime, especially when growing and dividing, cells go through various steps of the cell cycle. Knowledge of the individual steps of the cell cycle will help us understand the development of a variety of diseases better, including cancer, and also to design new drugs against it. New techniques for studying the molecular basis of these processes have recently been developed and are described in detail in this manual.
A glossary helps the reader to cope with the complex cell cycle terminology.

• Apoptosis
• DNA
• In situ Hybridization
• Kinasen
• Kinases
• Mitogenese
• Mitogenesis
• Viruses
• Zelltod
• Zellwachstum
• Zellzyklus
• hybridization
• protein
• transcription
• virus

"Newcomers to cell-cycle research will enjoy and profit greatly from reading this new manual." Trends in Cell Biology
"...a valuable desk reference for scientists and students. While several other books describing protocols for investigation of growth control have recently been published, this is among the most viable." FEBS Letters

• Mitotix, Cambridge, USA

  Michele Pagano

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