15N-NMR Spectroscopy

1. Front Matter

   Pages I-VII

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2. Introduction

   • Gérard J. Martin, Maryvonne L. Martin, Jean-Paul Gouesnard

   Pages 1-5

3. Relaxation Phenomena and Nuclear Overhauser Effects. Molecular Dynamics and Observation of the 15N Signals

   • Gérard J. Martin, Maryvonne L. Martin, Jean-Paul Gouesnard

   Pages 6-27

4. Experimental Techniques in 15N Spectroscopy

   • Gérard J. Martin, Maryvonne L. Martin, Jean-Paul Gouesnard

   Pages 28-43

5. Reference for 15N Chemical Shifts

   • Gérard J. Martin, Maryvonne L. Martin, Jean-Paul Gouesnard

   Pages 44-53

6. Medium Effects in 15N Spectroscopy

   • Gérard J. Martin, Maryvonne L. Martin, Jean-Paul Gouesnard

   Pages 54-74

7. 15N Chemical Shifts

   • Gérard J. Martin, Maryvonne L. Martin, Jean-Paul Gouesnard

   Pages 75-186

8. nJ15N ~ X Coupling Constants

   • Gérard J. Martin, Maryvonne L. Martin, Jean-Paul Gouesnard

   Pages 187-326

9. Application of 15N Spectroscopy to the Study of Dynamic Processes and Reaction Mechanisms

   • Gérard J. Martin, Maryvonne L. Martin, Jean-Paul Gouesnard

   Pages 327-341

10. Back Matter

    Pages 343-384

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After the proton and carbon, nitrogen is, with oxygen, the most impor­ tant atom in organic and especially bioorganic molecules. However, the development of nitrogen spectroscopy is indeed very recent. This is due to the fact that nitrogen-14, which is the naturally abundant iso­ tope, suffers, for structural studies, from the disadvantages inherent in nuclei with a quadrupolar moment (Table 1.1). Actually, indirect 15N measurements were reported in the early days of double resonance spectroscopy and the first direct detection of 15N resonance signals at the natural abundance level was realized in 1964 (R 17) at 4.33 MHz 1 (~ 1T) using a 15 mm o.d. cell in the field sweep mode (~ 0.16 min- ). Signal-to-noise ratios only of 3-4 were obtained for neat liquids and this low sensitivity of the 15N resonance still remains the main dis­ advantage for 15 spectroscopy (Table 1.1). However, nitrogen-15 has, N probably more than any other nucleus, benefited from the advances of NMR technology, i.e. Fourier transformation, multinuclear facilities, wide-bore super conducting solenoids, and, with the new generation of spectrometers, 15N-NMR is entering the field of routine investigation. Nevertheless, in spite of these spectacular improvements, obtaining 15N spectra of diluted species or large biochemical molecules is often not very easy and a good knowledge of the relaxation properties pecu­ liar to 15N may be necessary in order to adjust the pulse sequences and the decoupler duty cycle correctly (Section 2).

• Magnetische Kernresonanz
• carbon
• nuclear magnetic resonance
• nuclear magnetic resonance (NMR)
• oxygen
• purification
• spectroscopy

• Chimie Organique Physique, Faculté des Sciences, Université de Nantes, Nantes-Cedex, France

  Gérard J. Martin, Maryvonne L. Martin, Jean-Paul Gouesnard

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