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Rapid Cycle Real-Time PCR — Methods and Applications

Microbiology and Food Analysis

  • Book
  • © 2002

Overview

Editors:
  1. Udo Reischl

    1. Institut für Medizinische Mikrobiologie und Hygiene, Universitätsklinikum Regensburg, Regensburg, Germany

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  2. Carl Wittwer

    1. Department of Pathology, University of Utah Medical School, Salt Lake City, USA

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    You can also search for this editor in PubMed   Google Scholar

  3. Franklin Cockerill

    1. Division of Clinical Microbiology, Mayo Clinic, Rochester, USA

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    You can also search for this editor in PubMed   Google Scholar

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Table of contents (25 chapters)

  1. Front Matter

    Pages I-IX
    Download chapter PDF

  2. Introduction for Microbiology Volume of Rapid Cycle Real-Time PCR

    1. Introduction for Microbiology Volume of Rapid Cycle Real-Time PCR

      • Franklin Cockerill
      Pages 1-2

  3. Applications and Challenges of Real-Time PCR for the Clinical Microbiology Laboratory

    1. Applications and Challenges of Real-Time PCR for the Clinical Microbiology Laboratory

      • Franklin R. Cockerill III, James R. Uhl
      Pages 3-27

  4. Bacteriology

    1. Front Matter

      Pages 29-30
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    2. Rapid Detection and Simultaneous Differentiation of Bordetella pertussis and Bordetella parapertussis in Clinical Specimens by LightCycler PCR

      • Udo Reischl, Katrin Kösters, Birgit Leppmeier, Hans-Jörg Linde, Norbert Lehn
      Pages 31-43

    3. Rapid Detection and Simultaneous Differentiation of Legionella spp. and L. pneumophila in Potable Water Samples and Respiratory Specimens by LightCycler PCR

      • Nele Wellinghausen, Olfert Landt, Udo Reischl
      Pages 45-57

    4. Homogeneous Assays for the Rapid PCR detection of Burkholderia pseudomallei 16S rRNA gene on a Real-Time Fluorometric Capillary Thermocycler

      • Eric P.-H. Yap, Soon-Meng Ang, Shirley G.-K. Seah, Seng-Meng Phang
      Pages 59-69

    5. Rapid Detection of Toxigenic Corynebacterium diphtheriae by LightCycler PCR

      • Udo Reischl, Elena Samoilovich, Valentina Kolodkina, Norbert Lehn, Hans-Jörg Linde
      Pages 71-81

    6. Rapid Detection of Resistance Associated Mutations in Mycobacterium tuberculosis by LightCycler PCR

      • Maria J. Torres, Antonio Criado, Jose C. Palomares, Javier Aznar
      Pages 83-91

    7. Duplex LightCycler PCR Assay for the Rapid Detection of Methicillin-Resistant Staphylococcus aureus and Simultaneous Species Confirmation

      • Udo Reischl, Hans-Jörg Linde, Birgit Leppmeier, Norbert Lehn
      Pages 93-105

    8. Rapid Detection of Group B Streptococci Using the LightCycler Instrument

      • Danbing Ke, Christian Ménard, François J. Picard, Michel G. Bergeron
      Pages 107-114

    9. Rapid Detection and Quantification of Chlamydia trachomatis in Clinical Specimens by LightCycler PCR

      • Heidi Wood, Udo Reischl, Rosanna W. Peeling
      Pages 115-132

    10. Quantification of Toxoplasma gondii in Amniotic Fluid by Rapid Cycle Real-Time PCR

      • François Delhommeau, François Forestier
      Pages 133-138

    11. Genospecies-Specific Melting Temperature of the recA PCR Product for the Detection of Borrelia burgdorferi sensu lato and Differentiation of Borrelia garinii from Borrelia afzelii and Borrelia burgdorferi sensu stricto

      • Johanna Mäkinen, Qiushui He, Matti K. Viljanen
      Pages 139-147

    12. Rapid and Specific Detection of Coxiella burnetii by LightCycler PCR

      • Markus Stemmler, Hermann Meyer
      Pages 149-154

    13. Molecular Identification of Campylobacter coli and Campylobacter jejuni Using Automated DNA Extraction and Real-Time PCR with Species-Specific TaqMan Probes

      • Lynn A. Cooper, Luke T. Daum, Robert Roudabush, Kenton L. Lohman
      Pages 155-159

    14. Rapid and Specific Detection of Plesiomonas shigelloides Directly from Stool by LightCycler PCR

      • Jin Phang Loh, Eric Peng Huat Yap
      Pages 161-169

    15. Quantification of Thermostable Direct Hemolysin-Producing Vibrio Parahaemolyticus from Foods Assumed to Cause Food Poisoning Using the LightCycler Instrument

      • Yoshito Iwade, Akinori Yamauchi, Akira Sugiyama, Osamu Nakayama, Norichika H. Kumazawa
      Pages 171-176

  5. Detection of Fungal Pathogens

    1. Front Matter

      Pages 177-177
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    2. Quantification and Speciation of Fungal DNA in Clinical Specimens Using the LightCycler Instrument

      • Juergen Loeffler, Holger Hebart, Norbert Henke, Kathrin Schmidt, Hermann Einsele
      Pages 179-186
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Keywords

About this book

Rapid Cycle Real-Time PCR is a powerful analytical tool with broad application for the basic and applied life sciences. Compared with conventional PCR technology, Rapid Cycle Real-Time PCR is faster, has greater specificity, and is more easily adaptable for a variety of diagnostic tests, including qualitative, quantitative and mutation detection assays. This book provides general overviews of this technology for use in the clinical microbiology laboratory as well as specific diagnostic protocols for the detection of viral, bacterial and fungal pathogens and genetically modified organisms in human specimens and foodstuffs. All of these protocols have been developed, verified, and validated by experts in the field and should be of great interest for clinical microbiologists, pathologists, laboratory technologists as well as practicing physicians.

Editors and Affiliations

  • Institut für Medizinische Mikrobiologie und Hygiene, Universitätsklinikum Regensburg, Regensburg, Germany

    Udo Reischl

  • Department of Pathology, University of Utah Medical School, Salt Lake City, USA

    Carl Wittwer

  • Division of Clinical Microbiology, Mayo Clinic, Rochester, USA

    Franklin Cockerill

Bibliographic Information

  • Book Title: Rapid Cycle Real-Time PCR — Methods and Applications

  • Book Subtitle: Microbiology and Food Analysis

  • Editors: Udo Reischl, Carl Wittwer, Franklin Cockerill

  • DOI: https://doi.org/10.1007/978-3-642-48351-6

  • Publisher: Springer Berlin, Heidelberg

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer-Verlag Berlin Heidelberg 2002

  • Softcover ISBN: 978-3-642-48353-0Published: 12 December 2012

  • eBook ISBN: 978-3-642-48351-6Published: 06 December 2012

  • Edition Number: 1

  • Number of Pages: IX, 258

  • Topics: Molecular Medicine

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