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Proteolytic Enzymes

Tools and Targets

  • Book
  • © 1999

Overview

Editors:
  1. Erwin E. Sterchi

    1. Institut für Biochemie und Molekularbiologie, Universität Bern, Bern, Germany

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    You can also search for this editor in PubMed   Google Scholar

  2. Walter Stöcker

    1. Institut für Zoophysiologie, Westfälische Wilhelms-Universität Münster, Münster, Germany

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • step-by-step protocols including practical hints and comprehensive theoretical background
  • Includes supplementary material: sn.pub/extras

Part of the book series: Springer Lab Manuals (SLM)

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Table of contents (18 chapters)

  1. Front Matter

    Pages I-IX
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  2. Introduction: Nomenclature and Classes of Peptidases

    1. Introduction: Nomenclature and Classes of Peptidases

      • A. J. Kenny
      Pages 1-8

  3. The Assay and Inhibition of Proteolytic Enzymes

    1. Front Matter

      Pages 9-9
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    2. Chromogenic Peptide Substrates

      • H. Kirschke, B. Wiederanders
      Pages 11-29

    3. Fluorometric Assays

      • David S. Auld
      Pages 30-48

    4. Detection of Proteolytic Enzymes Using Protein Substrates

      • Kevin K. W. Wang
      Pages 49-62

    5. Zymography, Casein Zymography, and Reverse Zymography: Activity Assays for Proteases and their Inhibitors

      • Gary W. Oliver, William G. Stetler-Stevenson, David E. Kleiner
      Pages 63-76

    6. Genetic - Based Assays of Viral Proteases

      • C. Rizzo, Y.-S. E. Cheng, B. Korant
      Pages 77-89

    7. Strategies for Inhibiting Proteases of Unknown Mechanism

      • Russell L. Wolz
      Pages 90-106

  4. Purification, Structure, Kinetics and Expression of Proteolytic Enzymes

    1. Front Matter

      Pages 107-107
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    2. Purification of Proteases

      • Nigel M. Hooper
      Pages 109-123

    3. Crystallization of Proteinases

      • Margit M. T. Bauer, Milton T. Stubbs
      Pages 124-147

    4. Basic Kinetic Mechanisms of Proteolytic Enzymes

      • L. Polgár
      Pages 148-166

    5. Kinetic Analysis of Protease Inhibition by Synthetic Inhibitors

      • C. Graham Knight
      Pages 167-187

    6. Phosphinic Peptide Libraries for Proteolytic Enzymes

      • V. Dive, J. Jiracek, A. Yiotakis
      Pages 188-198

    7. Functional Expression of Recombinant Proteases

      • Dieter Brömme, Brian F. Schmidt
      Pages 199-229

  5. The Use of Proteolytic Enzymes as Biochemical Tools

    1. Front Matter

      Pages 231-231
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    2. Proteases in Peptide Mapping and Sequencing

      • Josef Kellermann
      Pages 233-252

    3. Limited Proteolysis in the Study of Protein Conformation

      • Angelo Fontana, Patrizia Polverino de Laureto, Vincenzo de Filippis, Elena Scaramella, Marcello Zambonin
      Pages 253-280

    4. Limited Proteolysis in the Study of Membrane Proteins

      • Hassan Y. Naim
      Pages 281-297
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Keywords

About this book

Researchers may find themselves confronted with proteases, either because they play an essential role in a particular process they are studying, or because they interfere with that process. In either case they may need to investigate or inhibit the proteolytic activity. Others may wish to use proteolytic enzymes as laboratory tools.
This book has been written with these investigators in mind and includes assay methods using natural and artificial substrates, genetic-based assays, and strategies for the inhibition, purification and crystallization of proteases. In selected chapters the use of proteolytic enzymes to analyze proteins, segregate cells or in peptide synthesis is covered.

Editors and Affiliations

  • Institut für Biochemie und Molekularbiologie, Universität Bern, Bern, Germany

    Erwin E. Sterchi

  • Institut für Zoophysiologie, Westfälische Wilhelms-Universität Münster, Münster, Germany

    Walter Stöcker

Bibliographic Information

  • Book Title: Proteolytic Enzymes

  • Book Subtitle: Tools and Targets

  • Editors: Erwin E. Sterchi, Walter Stöcker

  • Series Title: Springer Lab Manuals

  • DOI: https://doi.org/10.1007/978-3-642-59816-6

  • Publisher: Springer Berlin, Heidelberg

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer-Verlag Berlin Heidelberg 1999

  • Softcover ISBN: 978-3-642-47807-9Published: 23 August 2014

  • eBook ISBN: 978-3-642-59816-6Published: 11 November 2013

  • Edition Number: 1

  • Number of Pages: IX, 366

  • Number of Illustrations: 40 b/w illustrations, 2 illustrations in colour

  • Topics: Biochemistry, general, Cell Biology

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