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Genetic Library Construction and Screening

Advanced Techniques and Applications

  • Book
  • © 2002

Overview

Editors:
  1. R. Curtis Bird

    1. Department of Pathobiology, Auburn University, Auburn, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Bruce F. Smith

    1. Scott-Ritchey Research Center, Auburn University, Auburn, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • A comprehensive, up-to-date guide on all aspects of synthesis and screening of genetic libraries
  • Detailed step-by-step protocols and extensive hands-on advice gleaned from the authors' experience
  • Perfectly suited for experienced researchers and graduate students using these technologies in the lab
  • Includes supplementary material: sn.pub/extras

Part of the book series: Springer Lab Manuals (SLM)

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Table of contents (11 chapters)

  1. Front Matter

    Pages I-XIII
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  2. PCR and cDNA Cloning Techniques

    1. Front Matter

      Pages 1-1
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    2. Strategies for cDNA Cloning and Mapping RNA Transcripts

      • Peter S. Silverstein, Shilpa J. Buch, R. Curtis Bird
      Pages 3-19

    3. Cloning PCR Products

      • Weiwen Jiang, Bruce F. Smith
      Pages 21-32

    4. Site Directed Deletion, Insertion, and Substitution using PCR

      • Michael Mingfu Ling, Brian H. Robinson
      Pages 33-53

    5. Long RT-PCR Cloning — Amplification of Full-Length Enterovirus Genomes

      • Kathleen Simpson, John W. Gow
      Pages 55-68

    6. Construction of cDNA Libraries from Small Quantities of Total RNA Using Template Switching Catalyzed by M-MLV Reverse Transcriptase

      • York Y. Zhu, Alex Chenchik, Roger Li, Florence Y. Hsieh, Paul D. Siebert
      Pages 69-93

  3. Subtractive and Differential Display Techniques

    1. Front Matter

      Pages 95-95
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    2. Subtractive Hybridization and cDNA Cloning

      • R. Curtis Bird, Gin Wu
      Pages 97-125

    3. Differential Display to Identify Steroid-Induced Genes in Endocrine Cells

      • Robert J. Kemppainen, Ellen N. Behrend
      Pages 127-142

  4. Specialized Cloning and Library Screening Strategies

    1. Front Matter

      Pages 143-143
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    2. Two Hybrid cDNA Cloning

      • Jörg A. Schenk
      Pages 145-165

    3. Yeast One and Two Hybrid cDNA Cloning

      • Betty C. B. Huang, Ying Luo
      Pages 167-185

    4. High-Throughput Library Screening by Fluorescent Hybridizations on Gridded Membranes

      • Hugues Roest Crollius, John O’brien, Hans Lehrach
      Pages 187-207

    5. Identification of Cell Targeting Ligands Using Random Peptide-Presenting Phage Libraries

      • Tatiana I. Samoylova, Bruce F. Smith
      Pages 209-231

  5. Back Matter

    Pages 233-241
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Keywords

Editors and Affiliations

  • Department of Pathobiology, Auburn University, Auburn, USA

    R. Curtis Bird

  • Scott-Ritchey Research Center, Auburn University, Auburn, USA

    Bruce F. Smith

Bibliographic Information

  • Book Title: Genetic Library Construction and Screening

  • Book Subtitle: Advanced Techniques and Applications

  • Editors: R. Curtis Bird, Bruce F. Smith

  • Series Title: Springer Lab Manuals

  • DOI: https://doi.org/10.1007/978-3-642-56408-6

  • Publisher: Springer Berlin, Heidelberg

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer-Verlag Berlin Heidelberg 2002

  • Softcover ISBN: 978-3-642-47733-1Published: 06 November 2012

  • eBook ISBN: 978-3-642-56408-6Published: 06 December 2012

  • Edition Number: 1

  • Number of Pages: XIII, 241

  • Topics: Cell Biology, Biochemistry, general, Cancer Research

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