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Analyzing Microbes

Manual of Molecular Biology Techniques

  • Book
  • © 2013

Overview

Editors:
  1. Dilip Kumar Arora

    1. National Bureau of Agriculturally Import, Maunath Bhanjan, India

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    You can also search for this editor in PubMed   Google Scholar

  2. Surajit Das

    1. , Dep. Life Sciences, National Institute of Technology, Rourkela, India

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    You can also search for this editor in PubMed   Google Scholar

  3. Mesapogu Sukumar

    1. National Bureau of Agriculturally Import, Maunath Bhanjan, India

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Provides step-by-step instructions for immediate use in the laboratory
  • With flow charts of all experiments
  • Includes suppliers list and recipes of solutions and media
  • Includes supplementary material: sn.pub/extras

Part of the book series: Springer Protocols Handbooks (SPH)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Microbial DNA Extraction, Purification, and Quantitation

    • Sukumar Mesapogu, Chandra Mouleswararao Jillepalli, Dilip K. Arora
    Pages 1-16

  3. Fluorescent-Based Detection, Quantitation, and Expression of Viral Gene by qRT-PCR

    • Shelly Praveen, Vikas Koundal
    Pages 17-29

  4. Restriction Enzymes and Their Role in Microbiology

    • Sukumar Mesapogu, Chandra Mouleswararao Jillepalli, Dilip K. Arora
    Pages 31-36

  5. Genetic Fingerprinting Techniques for Molecular Characterisation of Microbes

    • Annette Reineke, K. Uma Devi
    Pages 37-72

  6. Agarose Gel Electrophoresis and Polyacrylamide Gel Electrophoresis: Methods and Principles

    • Sukumar Mesapogu, Chandra Mouleswararao Jillepalli, Dilip K. Arora
    Pages 73-91

  7. Molecular Identification of Microbes: I. Macrophomina phaseolina

    • Bandamaravuri Kishore Babu, T. Kiran Babu, Rajan Sharma
    Pages 93-97

  8. Molecular Identification of Microbes: II. Bacillus

    • Anil Kumar Saxena
    Pages 99-104

  9. Molecular Identification of Microbes: III. Pseudomonas

    • Bhim Pratap Singh, Ratul Saikia
    Pages 105-112

  10. Molecular Identification of Microbes: IV. Vibrio

    • Hirak Ranjan Dash, Neelam Mangwani, Surajit Das
    Pages 113-122

  11. Molecular Identification of Microbes: V. methylotrophs

    • Kamlesh K. Meena, Manish Kumar, D. P. Singh
    Pages 123-134

  12. Preservation and Maintenance of Microbial Cultures

    • Sudheer Kumar, Prem L. Kashyap, Ruchi Singh, Alok K. Srivastava
    Pages 135-152

  13. Microbes from Extreme Environment: Molecular Identification Procedures

    • Surajit Das
    Pages 153-168

  14. ELISA-Based Identification and Detection of Microbes

    • Jyoti Verma, Sangeeta Saxena, Sunil G. Babu
    Pages 169-186

  15. Analysis of Microbial Diversity and Construction of Metagenomic Library

    • Thangamani Rajesh, Jeyaprakash Rajendhran, Paramasamy Gunasekaran
    Pages 187-208

  16. Bioinformatics Tools for Interpretation of Data Used in Molecular Identification

    • Suchi Smita, Krishna P. Singh, Bashir A. Akhoon, Shishir K. Gupta, Shailendra K. Gupta
    Pages 209-243

  17. Molecular Phylogenetics of Microbes

    • Surajit Das, Hirak Ranjan Dash
    Pages 245-260

  18. Microarray Technology: Basic Concept, Protocols, and Applications

    • P. P. Dubey, Dinesh Kumar
    Pages 261-279

  19. Microarray Analysis of Different Functional Genes of Microorganisms

    • Hirak Ranjan Dash, Surajit Das
    Pages 281-290

  20. DNA Cloning and Sequencing

    • Bandamaravuri Kishore Babu, Anu Sharma, Hari Kishan Sudini
    Pages 291-302
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Keywords

About this book

This Springer Protocols manual is a practical guide to the application of key molecular biology techniques in microbiological research. The focus is on experimental protocols, which are presented in an easy-to-follow way, as step-by-step procedures for direct use in the laboratory. Notes on how to successfully apply the procedures are included, as well as recommendations regarding materials and suppliers. In addition to the practical protocols, important background information and representative results of experiments using the described methods are presented. Researchers in all areas applying microbial systems, such as in molecular biology, genetics, pathology, and agricultural research will find this work of great value.

Reviews

From the reviews:

“The Analyzing Microbes­—Manual of Molecular Biology Techniques is indeed a practical guide to the application of various molecular biology techniques in general microbiological research, with special emphasis in/on biotechnology and environmental microbiology. … All chapters are written in a concise step-by-step algorithm and are easy to follow during reading or bench working. … In my opinion, the aim of this book … to produce a self-contained laboratory manual which will be useful to both experienced practitioners and beginners in the field, is fully accomplished.” (Ioan I. Ardelean, Romanian Journal of Biochemistry, Vol. 50 (1), 2013)

Editors and Affiliations

  • National Bureau of Agriculturally Import, Maunath Bhanjan, India

    Dilip Kumar Arora, Mesapogu Sukumar

  • , Dep. Life Sciences, National Institute of Technology, Rourkela, India

    Surajit Das

Bibliographic Information

  • Book Title: Analyzing Microbes

  • Book Subtitle: Manual of Molecular Biology Techniques

  • Editors: Dilip Kumar Arora, Surajit Das, Mesapogu Sukumar

  • Series Title: Springer Protocols Handbooks

  • DOI: https://doi.org/10.1007/978-3-642-34410-7

  • Publisher: Springer Berlin, Heidelberg

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer-Verlag Berlin Heidelberg 2013

  • Hardcover ISBN: 978-3-642-34409-1Published: 03 February 2013

  • Softcover ISBN: 978-3-662-50967-8Published: 24 September 2016

  • eBook ISBN: 978-3-642-34410-7Published: 03 February 2013

  • Series ISSN: 1949-2448

  • Series E-ISSN: 1949-2456

  • Edition Number: 1

  • Number of Pages: XI, 352

  • Number of Illustrations: 18 b/w illustrations, 62 illustrations in colour

  • Topics: Microbiology, Medical Microbiology, Biochemistry, general

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