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Artificial Nucleases

  • Book
  • © 2004

Overview

Editors:
  1. Marina A. Zenkova

    1. Novosibirsk Institute of Bioorganic Chemistry, Novosibirsk, Russia

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  • First book to present a concise overview of the rapidly developing field of artificial nucleases and their application
  • Includes supplementary material: sn.pub/extras

Part of the book series: Nucleic Acids and Molecular Biology (NUCLEIC, volume 13)

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Table of contents (15 chapters)

  1. Front Matter

    Pages I-XVI
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  2. Introduction

    • Valentin V. Vlassov, Marina A. Zenkova
    Pages 1-2

  3. DNA Hydrolysis: Mechanism and Reactivity

    • N. H. Williams
    Pages 3-17

  4. Active Site of Ribonuclease A

    • R. T. Raines
    Pages 19-32

  5. Structural Considerations Concerning Cleavage of RNA

    • R. Kierzek
    Pages 33-48

  6. Cleavage of RNA by Imidazole

    • V. V. Vlassov, A. V. Vlassov
    Pages 49-60

  7. Principles of Nucleic Acid Cleavage by Metal Ions

    • A. Dallas, A. V. Vlassov, S. A. Kazakov
    Pages 61-88

  8. Allosterically Controlled Ribozymes as Artificial Ribonucleases

    • M. Iyo, H. Kawasaki, M. Miyagishi, K. Taira
    Pages 89-109

  9. Small Ribonuclease Mimics

    • I. L. Kuznetsova, V. N. Sil’nikov
    Pages 111-128

  10. Copper Containing Nuclease Mimics: Synthetic Models and Biochemical Applications

    • S. Verma, S. G. Srivatsan, C. Madhavaiah
    Pages 129-150

  11. RNA-Cleaving Oligonucleotide-Peptide Conjugates

    • N. L. Mironova, D. V. Pyshnyi, E. M. Ivanova
    Pages 151-172

  12. Sequence Selective Artificial Ribonucleases Employing Metal Ions As Scissors

    • A. Kuzuya, R. Mizoguchi, M. Komiyama
    Pages 173-187

  13. Site-Specific Artificial Ribonucleases: Conjugates of Oligonucleotides with Catalytic Groups

    • M. A. Zenkova, N. G. Beloglazova
    Pages 189-221

  14. DNA and RNA Cleavage Mediated by Phenanthroline-Cuprous Oligonucleotides: From Properties to Applications

    • J.-C. Francois, M. Faria, D. Perrin, C. Giovannangeli
    Pages 223-242

  15. Sequence-Specific Cleavage of Double-Stranded DNA

    • A. S. Boutorine, P. B. Arimondo
    Pages 243-268

  16. Bleomycin-Oligonucleotide Conjugates as Site-Specific Nucleases

    • P. E. Vorobjev, V. F. Zarytova
    Pages 269-287

  17. Back Matter

    Pages 289-292
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Keywords

About this book

The development of agents capable of cleaving RNA and DNA has attracted considerable attention from researchers in the last few years, because of the immediate and very important applications they can find in the emerging fields of biotechnology and pharmacology. There are essentially two classes of these agents - nucleases that occur naturally inside cells and synthetically produced artificial nucleases. The first class includes protein enzyme nucle­ ases and catalytic RNA structured ribozymes that perform cleavage of the phosphodiester bonds in nucleic acids according to a hydrolytic pathway in the course of different biochemical processes in the cell. A different pathway is used by some antibiotics which cleave DNA via redox-based mechanisms resulting in oxidative damage of nucleotide units and breakage of the DNA backbone. The above molecules are indispensable tools for manipulating nucleic acids and processing RNA; DNA-cleaving antibiotics and cytotoxic ribonucleases have demonstrated utility as chemotherapeutic agents. The second class, artificial nucleases, are rationally designed to imitate the active centers of natural enzymes by simple structures possessing minimal sets of the most important characteristics that are essential for catalysis. A dif­ ferent approach, in vitro selection, was also used to create artificial RNA and DNA enzymes capable of cleaving RNA. Being less efficient and specific as compared to the natural enzymes, the primitive mimics are smaller and robust and can function in a broad range of conditions.

Editors and Affiliations

  • Novosibirsk Institute of Bioorganic Chemistry, Novosibirsk, Russia

    Marina A. Zenkova

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