Skip to main content
SpringerLink
Log in

Flow Cytometry and Cell Sorting

  • Book
  • © 2000

Overview

Editors:
  1. Andreas Radbruch

    1. Deutsches Rheuma-Forschungszentrum Berlin (DRFZ), Berlin, Germany

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Easy-to-follow step-by-step instructions for immediate use at the lab bench protocols with a uniform clear structure and layout, which allow the techniques to be successfully reproduced
  • All necessary practical details and notes as well as a troubleshooting section are included
  • Up-to-date methods written by experts in their fields
  • Includes supplementary material: sn.pub/extras

Part of the book series: Springer Lab Manuals (SLM)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 169.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (26 chapters)

  1. Front Matter

    Pages I-XXIII
    Download chapter PDF

  2. Flow Cytometry

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Operation of a Flow Cytometer

      • Christoph Göttlinger, Birgit Mechtold, Andreas Radbruch
      Pages 3-25

  3. Immunofluorescence

    1. Front Matter

      Pages 27-27
      Download chapter PDF

    2. Conjugation of Fluorochromes, Haptens, and Phycobiliproteins to Antibodies

      • Werner Müller
      Pages 29-37

    3. Immunofluorescence: Basic Considerations

      • Andreas Radbruch
      Pages 38-52

    4. Multicolor Immunofluorescence Analysis

      • Chia-Huei Chen, Kerstin Willmann, Dieter Recktenwald
      Pages 53-62

    5. Combined Intracellular and Surface Staining: Immunofluorescence of Cytokines in T Cells

      • Mario Assenmacher
      Pages 63-70

    6. Cytometry and Sorting of Live Cells According to Their Secreted Products

      • Rudolf A. Manz
      Pages 71-76

    7. Cytometry of Rare Surface Molecules by Magnetofluorescent Liposomes

      • Alexander Scheffold
      Pages 77-81

    8. Scatchard Analysis by Flow Cytometry

      • Robert F. Murphy
      Pages 82-85

    9. Microbead Assay for Quantification of Neuronal Adhesion Molecule Interaction by Flow Cytometry

      • Attila Tárnok, Ursel Nöhrenberg, Hans-Jürgen Vollmer, Stephan Schuhmacher
      Pages 86-97

  5. Cellular Activation and Biochemistry

    1. Front Matter

      Pages 125-125
      Download chapter PDF

    2. Cell Activation: Indo-1 Ratiometric Analysis of Intracellular Ionized Calcium

      • Manfred Kubbies
      Pages 127-139

    3. Detection and Purification of Rare Responders by Fixed-Time Flow Cytometry

      • Attila Tárnok, Henning Ulrich
      Pages 140-158

    4. Biochemical Parameters of Cell Function

      • Gregor Rothe, Günter Valet
      Pages 159-182
Back to top

Keywords

About this book

Flow cytometric analysis of molecular, biochemical, genetic and developmental parameters using cellular fluorescence techniques as well as fluorescence-activated (FACS) or magnetic (MACS) cell sorting technologies provide unique options for molecular and cellular biology. In recent years, these technologies have been considerably advanced.
In this second edition, all chapters have been updated according to the recent improvements and modifications. Further, new protocols have been added, such as on magnetic selection, magnetofluorescent liposomes, the cytometry of secreted products and microbead assays, as well as reporter gene assays for cytometry and cell selection. The recent technical developments allow diagnostic differentiation of cells according to specific gene expression, identification of rare disease-associated cells and isolation of well-defined cells at high purity for cell therapies.

Editors and Affiliations

  • Deutsches Rheuma-Forschungszentrum Berlin (DRFZ), Berlin, Germany

    Andreas Radbruch

Bibliographic Information

  • Book Title: Flow Cytometry and Cell Sorting

  • Editors: Andreas Radbruch

  • Series Title: Springer Lab Manuals

  • DOI: https://doi.org/10.1007/978-3-662-04129-1

  • Publisher: Springer Berlin, Heidelberg

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer-Verlag Berlin Heidelberg 2000

  • Softcover ISBN: 978-3-540-65630-2Published: 02 November 1999

  • eBook ISBN: 978-3-662-04129-1Published: 14 March 2013

  • Edition Number: 2

  • Number of Pages: XXIII, 355

  • Topics: Cell Biology, Biochemistry, general, Immunology

Publish with us

Policies and ethics

Back to top

Search

Navigation