Testing for Genetic Manipulation in Plants

1. Front Matter

   Pages I-XVII

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2. Selectable and Screenable Markers for Rice Transformation

   • R. M. Twyman, E. Stöger, A. Kohli, T. Capell, P. Christou

   Pages 1-17

3. Use of Green Fluorescent Protein to Detect Transformed Shoots

   • J. Mounlinier, G. Hahne

   Pages 19-30

4. Luciferase Gene Expressed in Plants, Not in Agrobacterium

   • S. L. Mankin

   Pages 31-36

5. Use of β-Glucuronidase to Show Dehydration and High-Salt Gene Expression

   • K. Nakashima, K. Yamaguchi-Shinozaki

   Pages 37-61

6. Methods for Detecting Genetic Manipulation in Grain Legumes

   • H.-J. Jacobsen, R. Greiner

   Pages 63-72

7. Elimination of Selectable Marker Genes from Transgenic Crops

   • A. P. Gleave

   Pages 73-93

8. GST-MAT Vector for the Efficient and Practical Removal of Marker Genes from Transgenic Plants

   • H. Ebinuma, K. Sugita, E. Matsunaga, S. Endo, K. Yamada

   Pages 95-117

9. Safety Assessment of Insect-Protected Crops: Testing the Feeding Value of Bt Corn and Cotton Varieties in Poultry, Swine and Cattle

   • B. Hammond, E. Stanisiewski, R. Fuchs, J. Astwood, G. Hartnell

   Pages 119-137

10. Safety Assessment of Genetically Modified Rice and Potatoes with Soybean Glycinin

    • K. Momma, W. Hashimoto, S. Utsumi, K. Murata

    Pages 139-151

11. Chromosomal and Genetic Aberrations in Transgenic Soybean

    • R. J. Singh

    Pages 153-168

12. Transgenic Barley (Hordeum vulgare L.) and Chromosomal Variation

    • M.-J. Cho, H. W. Choi, P. Bregitzer, S. Zhang, P. G. Lemaux

    Pages 169-188

13. Back Matter

    Pages 189-194

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Molecular Methods of Plant Analysis Concept of the Series The powerful recombinant DNA technology and related developments have had an enormous impact on molecular biology. Any treatment of plant analy­ sis must make use of these new methods. Developments have been so fast and the methods so powerful that the editors of Modern Methods of Plant Analy­ sis have now decided to rename the series Molecular Methods of Plant Analy­ sis. This will not change the general aims of the series, but best describes the thrust and content of the series as we go forward into the new millennium. This does not mean that all chapters a priori deal only with the methods of molecular biology, but rather that these methods are to be found in many chapters together with the more traditional methods of analysis which have seen recent advances. The numbering of the volumes of the series therefore continues on from 20, which is the most recently published volume under the title Modern Methods of Plant Analysis. As indicated for previous volumes, the methods to be found in Molecular Methods of Plant Analysis are described critically, with hints as to their limi­ tations, references to original papers and authors being given, and the chap­ ters written so that there is little need to consult other texts to carry out the All authors have been chosen because of their methods of analysis described.

• Expression
• Glycin
• Hordeum vulgare L.
• Lebensmittel
• Protein
• Samen
• Selectable Marker
• analysis
• beta-glucuronidase
• corn
• food
• gene expression
• genetic manipulation
• marker genes
• transgen
• genetic engineering

• Department of Horticulture, Viticulture and Oenology, University of Adelaide, Glen Osmond, Australia

  J. F. Jackson

• Erlangen, Germany

  H. F. Linskens

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