Advanced Fluorescence Reporters in Chemistry and Biology I

1. Front Matter

   Pages i-x

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2. General Aspects

   1. Front Matter

      Pages 1-1

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   2. Comparative Analysis of Fluorescence Reporter Signals Based on Intensity, Anisotropy, Time-Resolution, and Wavelength-Ratiometry

      • Alexander P. Demchenko

      Pages 3-24

3. Design of Organic Dyes

   1. Front Matter

      Pages 25-25

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   2. Optimized UV/Visible Fluorescent Markers

      • M. Sameiro T. Gonçalves

      Pages 27-64

   3. Long-Wavelength Probes and Labels Based on Cyanines and Squaraines

      • Leonid D. Patsenker, Anatoliy L. Tatarets, Ewald A. Terpetschnig

      Pages 65-104

   4. Two-Photon Absorption in Near-IR Conjugated Molecules: Design Strategy and Structure–Property Relations

      • Olga V. Przhonska, Scott Webster, Lazaro A. Padilha, Honghua Hu, Alexey D. Kachkovski, David J. Hagan et al.

      Pages 105-147

   5. Discovery of New Fluorescent Dyes: Targeted Synthesis or Combinatorial Approach?

      • Eunha Kim, Seung Bum Park

      Pages 149-186

4. Organic Dyes with Response Function

   1. Front Matter

      Pages 187-187

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   2. Physical Principles Behind Spectroscopic Response of Organic Fluorophores to Intermolecular Interactions

      • Vladimir I. Tomin

      Pages 189-223

   3. Organic Dyes with Excited-State Transformations (Electron, Charge, and Proton Transfers)

      • Cheng-Chih Hsieh, Mei-Lin Ho, Pi-Tai Chou

      Pages 225-266

   4. Dyes with Segmental Mobility: Molecular Rotors

      • Mark A. Haidekker, Matthew Nipper, Adnan Mustafic, Darcy Lichlyter, Marianna Dakanali, Emmanuel A. Theodorakis

      Pages 267-308

   5. Electrochromism and Solvatochromism in Fluorescence Response of Organic Dyes: A Nanoscopic View

      • Patrik R. Callis

      Pages 309-330

   6. Electric Field Sensitive Dyes

      • Ronald J. Clarke

      Pages 331-344

5. Fluorophores of Visible Fluorescent Proteins

   1. Front Matter

      Pages 345-345

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6. Fluorophores of visible Fluorescent Proteins

   1. Photophysics and Spectroscopy of Fluorophores in the Green Fluorescent Protein Family

      • Fabienne Merola, Bernard Levy, Isabelle Demachy, Helene Pasquier

      Pages 347-383

7. Back Matter

   Pages 385-389

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Fluorescence reporter is the key element of any sensing or imaging technology. Its optimal choice and implementation is very important for increasing the sensitivity, precision, multiplexing power, and also the spectral, temporal, and spatial reso- tion in different methods of research and practical analysis. Therefore, design of ?uorescence reporters with advanced properties is one of the most important problems. In this volume, top experts in this ?eld provide advanced knowledge on the design and properties of ?uorescent dyes. Organic dyes were the ?rst ?uorescent materials used for analytical purposes, and we observe that they retain their leading positions against strong competition of new materials – conjugated polymers, semiconductor nanocrystals, and metal chelating complexes. Recently, molecular and cellular biology got a valuable tool of organic ?uorophores synt- sized by cell machinery and incorporated into green ?uorescent protein and its analogs. Demands of various ?uorescence techniques operating in spectral, anisotropy, and time domains require focused design of ?uorescence reporters well adapted to these techniques. Near-IR spectral range becomes more and more attractive for various applications, and new dyes emitting in this range are strongly requested. Two-photonic ?uorescence has become one of the major tools in bioimaging, and ?uorescence reporters well adapted to this technique are in urgent need. These problems cannot be solved without the knowledge of fundamental principles of dye design and of physical phenomena behind their ?uorescence response.

• Fluorophore
• UV/VIS
• excited states
• fluorescence
• fluorescence sensors
• imaging
• intermolecular interactions
• organic dyes
• organic photochemistry
• proteins

• , Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kiev, Ukraine

  Alexander P. Demchenko

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