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Antibody Engineering Volume 2

  • Book
  • © 2010

Overview

Editors:
  1. Roland Kontermann

    1. (Biomedical Engineering) Institut für Zellbiologie und Immunologie, Universität Stuttgart, Stuttgart, Germany

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  2. Stefan Dübel

    1. Technische Universität Braunschweig, Institut für Biochemie und Biotechnologie, Germany

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • The updated second edition of the successful Springer Lab Manual "Antibody Engineering" is now offered in two volumes with nearly the double number of protocols
  • With both new and well established techniques
  • Detailed step-by-step protocols including all materials and practical advices
  • Includes supplementary material: sn.pub/extras

Part of the book series: Springer Protocols Handbooks (SPH)

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Table of contents (39 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. Bioinformatics of Antigen-binding Sites

    1. Front Matter

      Pages 1-1
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    2. Analysis of Single Chain Antibody Sequences Using the VBASE2 Fab Analysis Tool

      • Svetlana Mollova, Ida Retter, Michael Hust, Stefan Dübel, Werner Müller
      Pages 3-10

    3. Standardized Sequence and Structure Analysis of Antibody Using IMGT®

      • François Ehrenmann, Patrice Duroux, Véronique Giudicelli, Marie-Paule Lefranc
      Pages 11-31

    4. Protein Sequence and Structure Analysis of Antibody Variable Domains

      • Andrew C. R. Martin
      Pages 33-51

  3. Generation of Antibody Fragments and Their Derivatives

    1. Front Matter

      Pages 54-54
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    2. scFv by Two-Step Cloning

      • Dafne Müller
      Pages 55-59

    3. Bivalent Diabodies

      • Roland E. Kontermann
      Pages 61-68

    4. Generation of Single-Chain Fv Fragments and Multivalent Derivatives scFv-Fc and scFv-CH3 (Minibodies)

      • Tove Olafsen, Vania E. Kenanova, Anna M. Wu
      Pages 69-84

    5. Miniantibodies

      • Jonas V. Schaefer, Peter Lindner, Andreas Plückthun
      Pages 85-99

    6. Generation of Stably Transfected Eukaryotic Cell Lines Producing ImmunoRNAse Fusion Proteins

      • Athanasios Mavratzas, Evelyn Exner, Jürgen Krauss, Michaela A. E. Arndt
      Pages 101-112

    7. Antibody–Cytokine Fusion Proteins with Members of the TNF-Family

      • Dafne Müller, Jeannette Gerspach
      Pages 113-126

    8. Recombinant Immunotoxins for Treating Cancer

      • Ira Pastan, Mitchell Ho
      Pages 127-146

    9. T Bodies

      • Bianca Altvater, Silke Landmeier, Claudia Rossig
      Pages 147-159

    10. Expressing Intrabodies in Mammalian Cells

      • Alessio Cardinale, Silvia Biocca
      Pages 161-172

    11. Phenotypic Knockdown with Intrabodies

      • Nina Strebe, Manuela Schüngel
      Pages 173-180

    12. Disulfide-Stabilized Fv Fragments

      • Ulrich Brinkmann
      Pages 181-189

    13. PEGylation of Antibody Fragments to Improve Pharmacodynamics and Pharmacokinetics

      • Arutselvan Natarajan, Sally J. DeNardo
      Pages 191-205

    14. Fusion Proteins with Improved PK

      • Roland Stork
      Pages 207-218

    15. In Vivo Biotinylated scFv Fragments

      • Laila Al-Halabi, Torsten Meyer
      Pages 219-226
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Keywords

About this book

Antibodies are indispensable tools for research, diagnosis, and therapy. Recombinant approaches allow the modification and improvement of nearly all antibody properties, such as affinity, valency, specificity, stability, serum half-life, effector functions, and immunogenicity. "Antibody Engineering" provides a comprehensive toolbox covering the well-established basics but also many exciting new techniques. The protocols reflect the latest "hands on" knowledge of key laboratories in this still fast-moving field. Newcomers will benefit from the proven step-by-step protocols, which include helpful practical advice; experienced antibody engineers will appreciate the new ideas and approaches. The book is an invaluable resource for all those engaged in antibody research and development.

Editors and Affiliations

  • (Biomedical Engineering) Institut für Zellbiologie und Immunologie, Universität Stuttgart, Stuttgart, Germany

    Roland Kontermann

  • Technische Universität Braunschweig, Institut für Biochemie und Biotechnologie, Germany

    Stefan Dübel

Bibliographic Information

  • Book Title: Antibody Engineering Volume 2

  • Editors: Roland Kontermann, Stefan Dübel

  • Series Title: Springer Protocols Handbooks

  • DOI: https://doi.org/10.1007/978-3-642-01147-4

  • Publisher: Springer Berlin, Heidelberg

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer-Verlag GmbH Germany, part of Springer Nature 2010

  • Hardcover ISBN: 978-3-642-01146-7Due: 26 March 2010

  • Softcover ISBN: 978-3-642-42276-8Published: 23 September 2014

  • eBook ISBN: 978-3-642-01147-4Published: 10 March 2010

  • Series ISSN: 1949-2448

  • Series E-ISSN: 1949-2456

  • Edition Number: 2

  • Number of Pages: XII, 589

  • Additional Information: Originally published in one volume, within the series "Springer Lab Manuals"

  • Topics: Immunology, Antibodies

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