Single Molecules and Nanotechnology

1. Front Matter

   Pages i-xiii

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2. Nanophotonics and Single Molecules

   • W. E. Moerner, P. James Schuck, David P. Fromm, Anika Kinkhabwala, Samuel J. Lord, Stefanie Y. Nishimura et al.

   Pages 1-23

3. Single Biomolecules at Cryogenic Temperatures: From Structure to Dynamics

   • Clemens Hofmann, Florian Kulzer, Rob Zondervan, Jürgen Köhler, Michel Orrit

   Pages 25-51

4. Quantum Optics: Colloidal Fluorescent Semiconductor Nanocrystals (Quantum Dots) in Single-Molecule Detection and Imaging

   • Laurent A. Bentolila, Xavier Michalet, Shimon Weiss

   Pages 53-81

5. Scanning Fluorescence Correlation Spectroscopy

   • Zdeněk Petrášek, Petra Schwille

   Pages 83-105

6. Single-Molecule Imaging of Cellular Signaling

   • Sandra De Keijzer, B. Ewa Snaar-Jagalska, Herman P. Spaink, Thomas Schmidt

   Pages 107-129

7. Mobility and Signaling of Single Receptor Proteins

   • Michael Prummer, Horst Vogel

   Pages 131-162

8. Single Enzyme Kinetics: A Study of the Yeast Enzyme Candida Antarctica Lipase B

   • Wendy Verheijen, Davey Loos, Frans C. De Schryver, Johan Hofkens

   Pages 163-180

9. Protein Folding and Dynamics from Optical Single Molecule Spectroscopy

   • Benjamin Schuler, Gilad Haran

   Pages 181-215

10. Single Molecules and Nanoscale Surfactant Networks

    • Aldo Jesorka, Michal Tokarz, Owe Orwar

    Pages 217-250

11. Single-Molecule Covalent Chemistry in a Protein Nanoreactor

    • Hagan Bayley, Tudor Luchian, Seong-Ho Shin, Mackay B. Steffensen

    Pages 251-277

12. Single-Molecule Microscopy and Force Spectroscopy of Membrane Proteins

    • Andreas Engel, Harald Janovjak, Dimtrios Fotiadis, Alexej Kedrov, David Cisneros, Daniel J. Müller

    Pages 279-311

13. Back Matter

    Pages 313-318

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The investigation of molecules as individuals has grown rapidly in recent years, and in the process has uncovered molecular properties not normally accessible by ensemble experiments. In particular, the direct characterization of biologically important molecules such as enzymes, molecular motors, or receptors and entire signaling complexes in action, for example in a live biological cell, yielded un- pected insights. Common approaches for studying single molecules include the electrical detection of ion channels in membranes, the measurement of the dynamics of (bio)chemical reactions between individual molecules, the imaging of individual molecules by scanning probe techniques or by fluorescence correlation spectr- copy, and the direct monitoring of single molecules by optical microscopies, to mention a few. The application of these techniques in physics, chemistry, and bi- ogy has opened new areas of nanotechnology. This book provides a representative selection of recent developments in the rapidly evolving field of single molecule techniques of importance in life sciences and will have future impact on the quan- tative description of biological processes. The editors of this book hope that the chapters, written by leading scientists in the field, will attract students and scientists from different disciplines, provide them with an authentic insight into this young field of research, allow them to evaluate experimental methods and results, and thereby give them support for their own research. Lausanne Rudolf Rigler September 2007 Horst Vogel v Contents 1 Nanophotonics and Single Molecules. . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 W. E. Moerner, P. James Schuck, David P.

• Einzelmolekülfluoreszenzmikroskopie
• Einzelmolekülfluoreszenzspektroscopy
• cells
• enzyme kinetics
• enzymes
• fluorescence
• force microscopy
• imaging
• microscopy
• nanotechnology
• protein labelling
• single molecule chemistry
• single molecule fluorescence
• single molecule fluorescence microscopy
• temperature

• Laboratory of Medical Biophysics, MBB, Karolinska Institute, Stockolm, Sweden

  R. Rigler

• Laboratory of Physical Chemistry of Polymers and Membranes Institute of Chemical Sciences and Engineering, Swiss Federal Institute of Technology (EPFL), Lausanne, Switzerland

  H. Vogel

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