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Microinjection and Transgenesis

Strategies and Protocols

  • Book
  • © 1998

Overview

Editors:
  1. Angel Cid-Arregui

    1. Neurobiologie, Universität Heidelberg, Heidelberg, Germany

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    You can also search for this editor in PubMed   Google Scholar

  2. Alejandro García-Carrancá

    1. Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Mexico City, Mexico

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • A practical tool presenting the latest techniques developed in renowned laboratories
  • Easy to follow step-by-step protocols
  • Background information and full practical details (materials needed, exact recipes, troubleshooting guide, typical results, list of suppliers, etc.)

Part of the book series: Springer Lab Manuals (SLM)

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Table of contents (30 chapters)

  1. Front Matter

    Pages I-XIII
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  2. Strategies and Protocols for Microinjection Experiments Using Somatic Cells in Culture

    1. Front Matter

      Pages 1-1
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    2. Protocols for the Manual and Automatic Microinjection of Somatic Cells in Culture and for the Analysis of Microinjected Cells

      • Angel Cid-Arregui, Carla Santana, Miriam Guido, Nestor Morales-Peza, Maria Victoria Juarez, Roger Wepf et al.
      Pages 3-30

    3. Automated Computer-Assisted Microinjection into cultured somatic cells

      • Wilhelm Ansorge, Rainer Saffrich
      Pages 31-46

    4. Immunofluorescence and Immunoelectron Microscopy of Microinjected and Transfected Cultured Cells

      • Gustavo Egea, Teresa Babia, Roser Pagan, Roser Busca, Inmaculada Ayala, Ferran Valderrama et al.
      Pages 47-79

    5. Videomicroscopy of Living Microinjected Hippocampal Neurons in Culture

      • Frank Bradke, Carlos G. Dotti
      Pages 81-94

    6. Preparation, Purification, and Analysis of Synthetic Oligonucleotides Suitable for Microinjection Experiments

      • Ramon Güimil Garcia, Ramon Eritja
      Pages 95-112

    7. Colorimetric in Situ Hybridization

      • Jaime Berumen
      Pages 113-129

    8. Manual Microinjection: Measuring the Inhibitory Effect of HIV-1-Directed Hammerhead Ribozymes in Tissue Culture Cells

      • Robert Hormes, Georg Sczakiel
      Pages 131-143

    9. Cellular Microbiochemistry

      • Rainer Pepperkok, Olaf Rosorius, Jochen Scheel
      Pages 145-154

    10. A New Strategy for Studying Microtubule Transport and Assembly During Axon Growth

      • Peter W. Baas
      Pages 155-170

    11. The Use of Microinjection to Study Signal Transduction in Mammalian Cells

      • Serge Roche, Sara A. Courtneidge
      Pages 171-183

  3. Strategies and Protocols for Microinjection Experiments Using Embryos

    1. Front Matter

      Pages 185-185
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    2. Supply and Husbandry of Mice for Transgenic Science

      • Jane Morrell
      Pages 187-215

    3. Mouse Male Germ Cells in Culture: Toward a New Approach in Transgenesis?

      • Minoo Rassoulzadegan, Julien Sage, Valérie Grandjean
      Pages 217-227

    4. Sperm-Mediated Gene Transfer

      • Marialuisa Lavitrano, Valentina Lulli, Barbara Maione, Sabina Sperandio, Corrado Spadafora
      Pages 229-254

    5. Application of Ligand-Dependent Site-Specific Recombination in ES Cells

      • Pierre-Olivier Angrand, Catherine P. Woodroofe, A. Francis Stewart
      Pages 255-274

    6. A Modified Protocol for the Generation of Transgenic Mice and Freezing of Mouse Embryos

      • Carol Murphy, Luis Martin-Parras, Ulrich Rüther
      Pages 275-284

    7. The Use of Morula Aggregation to Generate Germline Chimeras from Genetically Modified Embryonic Stem Cells

      • Miguel Torres
      Pages 285-296

    8. The Generation of Transgenic Mice with Yeast Artificial Chromosomes

      • Holger Hiemisch, Thorsten Umland, Lluís Montoliu, Günther Schütz
      Pages 297-308
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Keywords

About this book

The establishment of microinjection protocols about 20 years ago for cultured cells and shortly thereafter for the generation of transgenic mice by microinjection of DNA into fertilized mouse eggs greatly influ­ enced many fields of biology. Not only have the data generated using these approaches contributed to a large extent to our present under­ standing of gene regulation and cellular function of higher eukaryotic cells, but current knowledge and future developments in this area will certainly have a great impact on basic and applied research for many years to come. This laboratory manual describes the current state of the art in this research area and focuses primarily on both the experimental strategies with an extensive bibliography and the detailed procedures. A large number of studies are presently being performed and a great variety of new experimental designs are rapidly being developed. The book con­ tains protocols on injection of somatic cells as well as on injection of embryos, the use of similar equipment being a common feature. In the articles dedicated to somatic cells, full descriptions of the manual and automatic injection systems are given as well as the methods for the analysis of injected cells by video-microscopy, electron microscopy or in situ hybridizations. In addition, comprehensive protocols are given for injection experiments with very different purposes, such as to study sig­ nal transduction or microtubule dynamics.

Editors and Affiliations

  • Neurobiologie, Universität Heidelberg, Heidelberg, Germany

    Angel Cid-Arregui

  • Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Mexico City, Mexico

    Alejandro García-Carrancá

Bibliographic Information

  • Book Title: Microinjection and Transgenesis

  • Book Subtitle: Strategies and Protocols

  • Editors: Angel Cid-Arregui, Alejandro García-Carrancá

  • Series Title: Springer Lab Manuals

  • DOI: https://doi.org/10.1007/978-3-642-80343-7

  • Publisher: Springer Berlin, Heidelberg

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer-Verlag Berlin Heidelberg 1998

  • Softcover ISBN: 978-3-540-61895-9Published: 27 November 1997

  • eBook ISBN: 978-3-642-80343-7Published: 06 December 2012

  • Edition Number: 1

  • Number of Pages: XIV, 619

  • Number of Illustrations: 56 b/w illustrations, 10 illustrations in colour

  • Topics: Cell Biology

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